Advanced

Substrate-dependent production and some properties of a thermostable, α-galactosidase from Rhodothermus marinus

Blücher, Anna ; Karlsson, Eva Nordberg LU and Holst, Olle LU (2000) In Biotechnology Letters 22(8). p.663-669
Abstract

α-Galactosidase activity in Rhodothermus marinus is dependent on the composition of the growth media. A maximum of 46 μU g-1 cell dry weight was obtained using minimal medium with galactooligo- or polysaccharides as single carbon source. An enzyme hydrolysing both high and low molecular weight galacto-saccharides was partly purified from the cell fractions. The molecular weight was 200 kDa (native) and 50 kDa (monomer). It was optimally active at 85°C, with a half-life of 2 h at 75°C, and had a broad pH range (4-8).

Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Galactosides, Glycosyl hydrolase, Thermophilic
in
Biotechnology Letters
volume
22
issue
8
pages
7 pages
publisher
Springer
external identifiers
  • scopus:17944404461
ISSN
0141-5492
DOI
10.1023/A:1005627501609
language
English
LU publication?
yes
id
54e85fd8-bf28-4267-836f-c0615eeaed58
date added to LUP
2018-11-14 21:17:02
date last changed
2020-04-29 03:46:31
@article{54e85fd8-bf28-4267-836f-c0615eeaed58,
  abstract     = {<p>α-Galactosidase activity in Rhodothermus marinus is dependent on the composition of the growth media. A maximum of 46 μU g<sup>-1</sup> cell dry weight was obtained using minimal medium with galactooligo- or polysaccharides as single carbon source. An enzyme hydrolysing both high and low molecular weight galacto-saccharides was partly purified from the cell fractions. The molecular weight was 200 kDa (native) and 50 kDa (monomer). It was optimally active at 85°C, with a half-life of 2 h at 75°C, and had a broad pH range (4-8).</p>},
  author       = {Blücher, Anna and Karlsson, Eva Nordberg and Holst, Olle},
  issn         = {0141-5492},
  language     = {eng},
  month        = {04},
  number       = {8},
  pages        = {663--669},
  publisher    = {Springer},
  series       = {Biotechnology Letters},
  title        = {Substrate-dependent production and some properties of a thermostable, α-galactosidase from Rhodothermus marinus},
  url          = {http://dx.doi.org/10.1023/A:1005627501609},
  doi          = {10.1023/A:1005627501609},
  volume       = {22},
  year         = {2000},
}