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Optimization of integrated chromatography sequences for purification of biopharmaceuticals

Löfgren, Anton LU orcid ; Yamanee-Nolin, Mikael LU ; Tallvod, Simon LU ; Fons, Joaquín G. LU ; Andersson, Niklas LU orcid and Nilsson, Bernt LU orcid (2019) In Biotechnology Progress 35(6).
Abstract

With continued development of integrated and continuous downstream purification processes, tuning and optimization become increasingly complicated with additional parameters and codependent variables over the sequence. This article offers a novel perspective of nonlinear optimization of integrated sequences with regard to individual column sizes, flow rates, and scheduling. The problem setup itself is a versatile tool to be used in downstream design which is demonstrated in two case studies: a four-column integrated sequence and a continuously loaded twin-capture setup with five columns.

Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
chromatography, continuous bioprocessing, integrated column sequences, multiple column design, optimization
in
Biotechnology Progress
volume
35
issue
6
article number
e2871
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:31207182
  • scopus:85068480067
ISSN
8756-7938
DOI
10.1002/btpr.2871
language
English
LU publication?
yes
id
54ea590d-d7e6-40ad-aaa5-1ccfdf510b43
date added to LUP
2019-07-17 14:08:54
date last changed
2025-10-14 13:18:32
@article{54ea590d-d7e6-40ad-aaa5-1ccfdf510b43,
  abstract     = {{<p>With continued development of integrated and continuous downstream purification processes, tuning and optimization become increasingly complicated with additional parameters and codependent variables over the sequence. This article offers a novel perspective of nonlinear optimization of integrated sequences with regard to individual column sizes, flow rates, and scheduling. The problem setup itself is a versatile tool to be used in downstream design which is demonstrated in two case studies: a four-column integrated sequence and a continuously loaded twin-capture setup with five columns.</p>}},
  author       = {{Löfgren, Anton and Yamanee-Nolin, Mikael and Tallvod, Simon and Fons, Joaquín G. and Andersson, Niklas and Nilsson, Bernt}},
  issn         = {{8756-7938}},
  keywords     = {{chromatography; continuous bioprocessing; integrated column sequences; multiple column design; optimization}},
  language     = {{eng}},
  number       = {{6}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Biotechnology Progress}},
  title        = {{Optimization of integrated chromatography sequences for purification of biopharmaceuticals}},
  url          = {{http://dx.doi.org/10.1002/btpr.2871}},
  doi          = {{10.1002/btpr.2871}},
  volume       = {{35}},
  year         = {{2019}},
}