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Comparing the effect of Cu-based fungicides and pure Cu salts on microbial biomass, microbial community structure and bacterial community tolerance to Cu

Vázquez-Blanco, Raquel ; Arias-Estévez, Manuel ; Bååth, Erland LU and Fernández-Calviño, David LU (2021) In Journal of Hazardous Materials 409.
Abstract

The effect of Cu on three different microbial endpoints was studied using different Cu sources, in order to check the usefulness of pure Cu salts to estimate the toxicity of commercial Cu fungicides on soil microbes. Cu additions caused similar dose-response curves of substrate induced respiration (SIR) decreases regardless of Cu source, i.e. the use of pure Cu salts to estimate the effect of Cu fungicides on microbial biomass using SIR may be useful. Phospholipid fatty acid (PLFA) analysis showed that the Cu source was more important for the microbial community structure than Cu concentration. Thus, the use of Cu salts to infer the effects of Cu fungicides on microbial community structure using PLFA analysis is not recommended, since... (More)

The effect of Cu on three different microbial endpoints was studied using different Cu sources, in order to check the usefulness of pure Cu salts to estimate the toxicity of commercial Cu fungicides on soil microbes. Cu additions caused similar dose-response curves of substrate induced respiration (SIR) decreases regardless of Cu source, i.e. the use of pure Cu salts to estimate the effect of Cu fungicides on microbial biomass using SIR may be useful. Phospholipid fatty acid (PLFA) analysis showed that the Cu source was more important for the microbial community structure than Cu concentration. Thus, the use of Cu salts to infer the effects of Cu fungicides on microbial community structure using PLFA analysis is not recommended, since effects of Cu concentration will be confounded with Cu source. Analyzing pollution induced community tolerance (PICT) to Cu showed that the use of pure Cu salts may overestimate Cu effects if Cu salt additions modified the soil pH. The highest doses of Cu salts increased bacterial community tolerance to Cu between 300 and 600 times, while commercial Cu fungicide increases were between 20 and 160 times. Therefore, the use of pure Cu salts to estimate the Cu fungicides effects on soil microbes is not recommended for PLFAs analyses, not suitable for PICT at high Cu concentrations, while useful for SIR.

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type
Contribution to journal
publication status
published
subject
keywords
Commercial Cu fungicides, PICT, PLFA, Pure salt copper, SIR
in
Journal of Hazardous Materials
volume
409
article number
124960
publisher
Elsevier
external identifiers
  • scopus:85099220002
  • pmid:33422757
ISSN
0304-3894
DOI
10.1016/j.jhazmat.2020.124960
language
English
LU publication?
yes
id
5509ca5f-6fe9-41df-831d-5a0ec739b07e
date added to LUP
2021-01-25 07:30:35
date last changed
2021-04-27 05:06:32
@article{5509ca5f-6fe9-41df-831d-5a0ec739b07e,
  abstract     = {<p>The effect of Cu on three different microbial endpoints was studied using different Cu sources, in order to check the usefulness of pure Cu salts to estimate the toxicity of commercial Cu fungicides on soil microbes. Cu additions caused similar dose-response curves of substrate induced respiration (SIR) decreases regardless of Cu source, i.e. the use of pure Cu salts to estimate the effect of Cu fungicides on microbial biomass using SIR may be useful. Phospholipid fatty acid (PLFA) analysis showed that the Cu source was more important for the microbial community structure than Cu concentration. Thus, the use of Cu salts to infer the effects of Cu fungicides on microbial community structure using PLFA analysis is not recommended, since effects of Cu concentration will be confounded with Cu source. Analyzing pollution induced community tolerance (PICT) to Cu showed that the use of pure Cu salts may overestimate Cu effects if Cu salt additions modified the soil pH. The highest doses of Cu salts increased bacterial community tolerance to Cu between 300 and 600 times, while commercial Cu fungicide increases were between 20 and 160 times. Therefore, the use of pure Cu salts to estimate the Cu fungicides effects on soil microbes is not recommended for PLFAs analyses, not suitable for PICT at high Cu concentrations, while useful for SIR.</p>},
  author       = {Vázquez-Blanco, Raquel and Arias-Estévez, Manuel and Bååth, Erland and Fernández-Calviño, David},
  issn         = {0304-3894},
  language     = {eng},
  publisher    = {Elsevier},
  series       = {Journal of Hazardous Materials},
  title        = {Comparing the effect of Cu-based fungicides and pure Cu salts on microbial biomass, microbial community structure and bacterial community tolerance to Cu},
  url          = {http://dx.doi.org/10.1016/j.jhazmat.2020.124960},
  doi          = {10.1016/j.jhazmat.2020.124960},
  volume       = {409},
  year         = {2021},
}