Inhibition of insulin release after passive transfer of immunoglobulin from insulin-dependent diabetic children to mice
Svenningsen, Annette ; Dyrberg, Thomas ; Gerling, Ivan ; Lernmark, Åke LU
; Mackay, Peter
and Rabinovitch, Alexander
(1983)
In Journal of Clinical Endocrinology and Metabolism
57(6).
p.1301-1304
- Abstract
We used the mouse passive transfer model to test whether islet cell antibodies affect β-cell function. The immunoglobulin (Ig) fraction of plasma from 5 islet cell surface antibody-positive, newly diagnosed insulin-dependent diabetic children or of a pool of plasma from 12 normal subjects was injected daily (7–16 mg IgG/day) for 14 days into normal immunosuppressed BALB/c mice. Insulin secretory responses in the Ig-injected mice were then examined by perfusing the rodent pancreata in vitro. Insulin release induced by 20 mmol/liter Dglucose during 30 min of stimulation decreased from 900 ng insulin (median; range, 814–1138) from pancreata of mice injected with control Ig to 511 ng (range, 130–786) from pancreata of mice injected with... (More)
We used the mouse passive transfer model to test whether islet cell antibodies affect β-cell function. The immunoglobulin (Ig) fraction of plasma from 5 islet cell surface antibody-positive, newly diagnosed insulin-dependent diabetic children or of a pool of plasma from 12 normal subjects was injected daily (7–16 mg IgG/day) for 14 days into normal immunosuppressed BALB/c mice. Insulin secretory responses in the Ig-injected mice were then examined by perfusing the rodent pancreata in vitro. Insulin release induced by 20 mmol/liter Dglucose during 30 min of stimulation decreased from 900 ng insulin (median; range, 814–1138) from pancreata of mice injected with control Ig to 511 ng (range, 130–786) from pancreata of mice injected with diabetic Ig (P < 0.003). Both the initial peak and the sustained second phase of glucose-stimulated insulin release were depressed in 4 of the 5 pancreata from mice injected with diabetic Ig. These results indicate that circulating antibodies in diabetic children may alter β-cell function and possibly contribute to the pathogenesis of insulin-dependent diabetes.
(Less)
- author
- Svenningsen, Annette
; Dyrberg, Thomas
; Gerling, Ivan
; Lernmark, Åke
LU
; Mackay, Peter
and Rabinovitch, Alexander
- publishing date
- 1983-01-01
- type
- Contribution to journal
- publication status
- published
- in
- Journal of Clinical Endocrinology and Metabolism
- volume
- 57
- issue
- 6
- pages
- 4 pages
- publisher
- Oxford University Press
- external identifiers
-
- pmid:6355141
- scopus:0021076479
- ISSN
- 0021-972X
- DOI
- 10.1210/jcem-57-6-1301
- language
- English
- LU publication?
- no
- id
- 550a5b64-0980-4913-860d-71dfa0f7a3f8
- date added to LUP
- 2019-09-16 12:43:56
- date last changed
- 2025-10-14 10:58:59
@article{550a5b64-0980-4913-860d-71dfa0f7a3f8,
abstract = {{<p>We used the mouse passive transfer model to test whether islet cell antibodies affect β-cell function. The immunoglobulin (Ig) fraction of plasma from 5 islet cell surface antibody-positive, newly diagnosed insulin-dependent diabetic children or of a pool of plasma from 12 normal subjects was injected daily (7–16 mg IgG/day) for 14 days into normal immunosuppressed BALB/c mice. Insulin secretory responses in the Ig-injected mice were then examined by perfusing the rodent pancreata in vitro. Insulin release induced by 20 mmol/liter Dglucose during 30 min of stimulation decreased from 900 ng insulin (median; range, 814–1138) from pancreata of mice injected with control Ig to 511 ng (range, 130–786) from pancreata of mice injected with diabetic Ig (P < 0.003). Both the initial peak and the sustained second phase of glucose-stimulated insulin release were depressed in 4 of the 5 pancreata from mice injected with diabetic Ig. These results indicate that circulating antibodies in diabetic children may alter β-cell function and possibly contribute to the pathogenesis of insulin-dependent diabetes.</p>}},
author = {{Svenningsen, Annette and Dyrberg, Thomas and Gerling, Ivan and Lernmark, Åke and Mackay, Peter and Rabinovitch, Alexander}},
issn = {{0021-972X}},
language = {{eng}},
month = {{01}},
number = {{6}},
pages = {{1301--1304}},
publisher = {{Oxford University Press}},
series = {{Journal of Clinical Endocrinology and Metabolism}},
title = {{Inhibition of insulin release after passive transfer of immunoglobulin from insulin-dependent diabetic children to mice}},
url = {{http://dx.doi.org/10.1210/jcem-57-6-1301}},
doi = {{10.1210/jcem-57-6-1301}},
volume = {{57}},
year = {{1983}},
}