Pinpointing oxidative modifications in proteins-recent advances in analytical methods
(2010) In Analytical Methods 2(11). p.1638-1650- Abstract
- Oxidation is one of the key degradation pathways in proteins and is of relevance to analyze in a wide variety of research disciplines. The types of different modifications occurring during oxidation by reactive oxygen species (ROS) are almost as many as the number of available analytical methods. Protein oxidation in biological samples has traditionally been analyzed by various protein carbonyl assays, sometimes combined with mass spectrometry (MS) for identification of the carbonylated proteins. MS is now increasingly used also for the determination of the exact position of the oxidation in the amino acid sequence, an approach that is aided by recent developments within the field of proteomics. The following review summarizes the effects... (More)
- Oxidation is one of the key degradation pathways in proteins and is of relevance to analyze in a wide variety of research disciplines. The types of different modifications occurring during oxidation by reactive oxygen species (ROS) are almost as many as the number of available analytical methods. Protein oxidation in biological samples has traditionally been analyzed by various protein carbonyl assays, sometimes combined with mass spectrometry (MS) for identification of the carbonylated proteins. MS is now increasingly used also for the determination of the exact position of the oxidation in the amino acid sequence, an approach that is aided by recent developments within the field of proteomics. The following review summarizes the effects of ROS on proteins, describes methods for labeling and separation of oxidized proteins in complex mixtures, and provides insight into various MS-based methods to localize the modifications within the protein primary structure. Pitfalls with the different techniques are given, as well as examples from various applications within biological studies, protein therapeutics, plant science, the food industry and industrial biotechnology. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1773824
- author
- Törnvall, Ulrika LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Methods
- volume
- 2
- issue
- 11
- pages
- 1638 - 1650
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000285024500001
- scopus:78650315984
- ISSN
- 1759-9660
- DOI
- 10.1039/c0ay00375a
- language
- English
- LU publication?
- yes
- id
- 55273ea8-d871-46a5-bbfb-7b71fd4dc8dc (old id 1773824)
- date added to LUP
- 2016-04-01 10:32:24
- date last changed
- 2022-02-10 03:07:41
@article{55273ea8-d871-46a5-bbfb-7b71fd4dc8dc,
abstract = {{Oxidation is one of the key degradation pathways in proteins and is of relevance to analyze in a wide variety of research disciplines. The types of different modifications occurring during oxidation by reactive oxygen species (ROS) are almost as many as the number of available analytical methods. Protein oxidation in biological samples has traditionally been analyzed by various protein carbonyl assays, sometimes combined with mass spectrometry (MS) for identification of the carbonylated proteins. MS is now increasingly used also for the determination of the exact position of the oxidation in the amino acid sequence, an approach that is aided by recent developments within the field of proteomics. The following review summarizes the effects of ROS on proteins, describes methods for labeling and separation of oxidized proteins in complex mixtures, and provides insight into various MS-based methods to localize the modifications within the protein primary structure. Pitfalls with the different techniques are given, as well as examples from various applications within biological studies, protein therapeutics, plant science, the food industry and industrial biotechnology.}},
author = {{Törnvall, Ulrika}},
issn = {{1759-9660}},
language = {{eng}},
number = {{11}},
pages = {{1638--1650}},
publisher = {{Royal Society of Chemistry}},
series = {{Analytical Methods}},
title = {{Pinpointing oxidative modifications in proteins-recent advances in analytical methods}},
url = {{http://dx.doi.org/10.1039/c0ay00375a}},
doi = {{10.1039/c0ay00375a}},
volume = {{2}},
year = {{2010}},
}