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Opto-Avoidance-Elevated Plus Maze protocol to study positive or negative valence upon optogenetic stimulation in the mouse brain

Serra, Gian Pietro ; Georges, François and Wallén-Mackenzie, Åsa LU orcid (2024) In STAR Protocols 5(4).
Abstract

The elevated plus maze (EPM) apparatus consists of two open arms that provide aversive spaces and two closed arms that provide protective and welcoming spaces. Here, we present a protocol to implement the classical EPM apparatus in a real-time optogenetic environment to address behavioral avoidance in mice. We describe steps for performing stereotaxic surgery, mouse manipulation, and experimental setup. Finally, we detail the procedures required for the Opto-Avoidance-EPM test in which the naturally preferred closed arms are selectively paired with optogenetic stimulation. For complete details on the use and execution of this protocol, please refer to Serra et al.1

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Behavior, Cognitive Neuroscience, Neuroscience
in
STAR Protocols
volume
5
issue
4
article number
103474
publisher
Cell Press
external identifiers
  • scopus:85211086966
  • pmid:39644496
ISSN
2666-1667
DOI
10.1016/j.xpro.2024.103474
language
English
LU publication?
yes
id
553ce367-9ec2-4e44-a2e3-0ade0407185c
date added to LUP
2025-01-17 11:29:54
date last changed
2025-07-05 01:45:42
@article{553ce367-9ec2-4e44-a2e3-0ade0407185c,
  abstract     = {{<p>The elevated plus maze (EPM) apparatus consists of two open arms that provide aversive spaces and two closed arms that provide protective and welcoming spaces. Here, we present a protocol to implement the classical EPM apparatus in a real-time optogenetic environment to address behavioral avoidance in mice. We describe steps for performing stereotaxic surgery, mouse manipulation, and experimental setup. Finally, we detail the procedures required for the Opto-Avoidance-EPM test in which the naturally preferred closed arms are selectively paired with optogenetic stimulation. For complete details on the use and execution of this protocol, please refer to Serra et al.<sup>1</sup></p>}},
  author       = {{Serra, Gian Pietro and Georges, François and Wallén-Mackenzie, Åsa}},
  issn         = {{2666-1667}},
  keywords     = {{Behavior; Cognitive Neuroscience; Neuroscience}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{4}},
  publisher    = {{Cell Press}},
  series       = {{STAR Protocols}},
  title        = {{Opto-Avoidance-Elevated Plus Maze protocol to study positive or negative valence upon optogenetic stimulation in the mouse brain}},
  url          = {{http://dx.doi.org/10.1016/j.xpro.2024.103474}},
  doi          = {{10.1016/j.xpro.2024.103474}},
  volume       = {{5}},
  year         = {{2024}},
}