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Enhancement of oogenesis/spermatogenesis in meagre Argyrosomus regius using a combination of temperature control and GnRHa treatments

Mylonas, Constantinos C.; Salone, Sara; Biglino, Tommaso; de Mello, Paulo H.; Fakriadis, Ioannis; Sigelaki, Irini and Duncan, Neil (2016) In Aquaculture 464. p.323-330
Abstract

A multiple GnRHa injection method for inducing spawning of high fecundity and quality in meagre was optimized. Specifically, the study (a) examined how long females would continue spawning in response to consecutive, weekly GnRHa injections, if maintained at the natural spawning temperature, and (b) evaluated whether males can produce adequate amounts of good quality sperm for the same period, with the assistance of a GnRHa therapy. Combined with stable temperatures (19–20 °C) that are prevalent during the early spawning season (April–May) in the Mediterranean Sea, each weekly GnRHa injection induced ~ 2 consecutive spawns per week for a period of 17 weeks. Fish spawned consistently 2 days after treatment and produced high fecundity and... (More)

A multiple GnRHa injection method for inducing spawning of high fecundity and quality in meagre was optimized. Specifically, the study (a) examined how long females would continue spawning in response to consecutive, weekly GnRHa injections, if maintained at the natural spawning temperature, and (b) evaluated whether males can produce adequate amounts of good quality sperm for the same period, with the assistance of a GnRHa therapy. Combined with stable temperatures (19–20 °C) that are prevalent during the early spawning season (April–May) in the Mediterranean Sea, each weekly GnRHa injection induced ~ 2 consecutive spawns per week for a period of 17 weeks. Fish spawned consistently 2 days after treatment and produced high fecundity and egg quality (i.e. fertilization, hatching and early larval development). Maximum mean total fecundity (± S.D.) obtained was 1,415,000 ± 149,000 eggs kg− 1 in 32 spawns, being the highest total season fecundity reported for meagre, and significantly higher than the estimated maximum potential fecundity for the species. At the same time, sperm of consistently high quality (i.e. initial motility, duration of motility and storage survival) was produced in response to treatment with multiple GnRHa implants, though towards the end of the experiment a significant reduction (ANOVA, P ≤ 0.05) in spermatozoa density was observed. This protocol is expected to enhance greatly commercial production of meagre. Statement of relevance The present study reports on an optimized protocol for the induction of consistent and repeated spawning in meagre, based on the use of weekly GnRHa injections combined with stable temperatures that are prevalent at the early spawning season in the Mediterranean Sea. This protocol produced eggs of high fecundity and quality (i.e. fertilization, hatching and early larval development) for a period of > 4 months, and it is expected to enhance greatly commercial production of meagre.

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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Argyrosomus regius, Egg quality, GnRHa, Induced spawning, Meagre, Reproduction
in
Aquaculture
volume
464
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:84978193838
  • wos:000383370300044
ISSN
0044-8486
DOI
10.1016/j.aquaculture.2016.07.006
language
English
LU publication?
no
id
557c1c50-db59-46ce-b04a-ab380893c2a2
date added to LUP
2016-10-25 11:32:11
date last changed
2017-01-01 08:37:30
@article{557c1c50-db59-46ce-b04a-ab380893c2a2,
  abstract     = {<p>A multiple GnRHa injection method for inducing spawning of high fecundity and quality in meagre was optimized. Specifically, the study (a) examined how long females would continue spawning in response to consecutive, weekly GnRHa injections, if maintained at the natural spawning temperature, and (b) evaluated whether males can produce adequate amounts of good quality sperm for the same period, with the assistance of a GnRHa therapy. Combined with stable temperatures (19–20 °C) that are prevalent during the early spawning season (April–May) in the Mediterranean Sea, each weekly GnRHa injection induced ~ 2 consecutive spawns per week for a period of 17 weeks. Fish spawned consistently 2 days after treatment and produced high fecundity and egg quality (i.e. fertilization, hatching and early larval development). Maximum mean total fecundity (± S.D.) obtained was 1,415,000 ± 149,000 eggs kg<sup>− 1</sup> in 32 spawns, being the highest total season fecundity reported for meagre, and significantly higher than the estimated maximum potential fecundity for the species. At the same time, sperm of consistently high quality (i.e. initial motility, duration of motility and storage survival) was produced in response to treatment with multiple GnRHa implants, though towards the end of the experiment a significant reduction (ANOVA, P ≤ 0.05) in spermatozoa density was observed. This protocol is expected to enhance greatly commercial production of meagre. Statement of relevance The present study reports on an optimized protocol for the induction of consistent and repeated spawning in meagre, based on the use of weekly GnRHa injections combined with stable temperatures that are prevalent at the early spawning season in the Mediterranean Sea. This protocol produced eggs of high fecundity and quality (i.e. fertilization, hatching and early larval development) for a period of &gt; 4 months, and it is expected to enhance greatly commercial production of meagre.</p>},
  author       = {Mylonas, Constantinos C. and Salone, Sara and Biglino, Tommaso and de Mello, Paulo H. and Fakriadis, Ioannis and Sigelaki, Irini and Duncan, Neil},
  issn         = {0044-8486},
  keyword      = {Argyrosomus regius,Egg quality,GnRHa,Induced spawning,Meagre,Reproduction},
  language     = {eng},
  month        = {11},
  pages        = {323--330},
  publisher    = {Elsevier},
  series       = {Aquaculture},
  title        = {Enhancement of oogenesis/spermatogenesis in meagre Argyrosomus regius using a combination of temperature control and GnRHa treatments},
  url          = {http://dx.doi.org/10.1016/j.aquaculture.2016.07.006},
  volume       = {464},
  year         = {2016},
}