TRIM28 repression of retrotransposon-based enhancers is necessary to preserve transcriptional dynamics in embryonic stem cells
(2013) In Genome Research 23(3). p.452-461- Abstract
- TRIM28 is critical for the silencing of endogenous retroviruses (ERVs) in embryonic stem (ES) cells. Here, we reveal that an essential impact of this process is the protection of cellular gene expression in early embryos from perturbation by cis-acting activators contained within these retroelements. In TRIM28-depleted ES cells, repressive chromatin marks at ERVs are replaced by histone modifications typical of active enhancers, stimulating transcription of nearby cellular genes, notably those harboring bivalent promoters. Correspondingly, ERV-derived sequences can repress or enhance expression from an adjacent promoter in transgenic embryos depending on their TRIM28 sensitivity in ES cells. TRIM28-mediated control of ERVs is therefore... (More)
- TRIM28 is critical for the silencing of endogenous retroviruses (ERVs) in embryonic stem (ES) cells. Here, we reveal that an essential impact of this process is the protection of cellular gene expression in early embryos from perturbation by cis-acting activators contained within these retroelements. In TRIM28-depleted ES cells, repressive chromatin marks at ERVs are replaced by histone modifications typical of active enhancers, stimulating transcription of nearby cellular genes, notably those harboring bivalent promoters. Correspondingly, ERV-derived sequences can repress or enhance expression from an adjacent promoter in transgenic embryos depending on their TRIM28 sensitivity in ES cells. TRIM28-mediated control of ERVs is therefore crucial not just to prevent retrotransposition, but more broadly to safeguard the transcriptional dynamics of early embryos. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3657224
- author
- Rowe, Helen M. ; Kapopoulou, Adamandia ; Corsinotti, Andrea ; Fasching, Liana LU ; Macfarlan, Todd S. ; Tarabay, Yara ; Viville, Stephane ; Jakobsson, Johan LU ; Pfaff, Samuel L. and Trono, Didier
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Genome Research
- volume
- 23
- issue
- 3
- pages
- 452 - 461
- publisher
- Cold Spring Harbor Laboratory Press (CSHL)
- external identifiers
-
- wos:000315806800005
- scopus:84874626481
- pmid:23233547
- ISSN
- 1549-5469
- DOI
- 10.1101/gr.147678.112
- language
- English
- LU publication?
- yes
- id
- 558d3dc2-6375-4957-a042-6b8eeedf0893 (old id 3657224)
- date added to LUP
- 2016-04-01 13:40:12
- date last changed
- 2022-03-29 08:37:38
@article{558d3dc2-6375-4957-a042-6b8eeedf0893, abstract = {{TRIM28 is critical for the silencing of endogenous retroviruses (ERVs) in embryonic stem (ES) cells. Here, we reveal that an essential impact of this process is the protection of cellular gene expression in early embryos from perturbation by cis-acting activators contained within these retroelements. In TRIM28-depleted ES cells, repressive chromatin marks at ERVs are replaced by histone modifications typical of active enhancers, stimulating transcription of nearby cellular genes, notably those harboring bivalent promoters. Correspondingly, ERV-derived sequences can repress or enhance expression from an adjacent promoter in transgenic embryos depending on their TRIM28 sensitivity in ES cells. TRIM28-mediated control of ERVs is therefore crucial not just to prevent retrotransposition, but more broadly to safeguard the transcriptional dynamics of early embryos.}}, author = {{Rowe, Helen M. and Kapopoulou, Adamandia and Corsinotti, Andrea and Fasching, Liana and Macfarlan, Todd S. and Tarabay, Yara and Viville, Stephane and Jakobsson, Johan and Pfaff, Samuel L. and Trono, Didier}}, issn = {{1549-5469}}, language = {{eng}}, number = {{3}}, pages = {{452--461}}, publisher = {{Cold Spring Harbor Laboratory Press (CSHL)}}, series = {{Genome Research}}, title = {{TRIM28 repression of retrotransposon-based enhancers is necessary to preserve transcriptional dynamics in embryonic stem cells}}, url = {{https://lup.lub.lu.se/search/files/3518081/4022922.pdf}}, doi = {{10.1101/gr.147678.112}}, volume = {{23}}, year = {{2013}}, }