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Uptake, depuration and sublethal effects of the neonicotinoid, imidacloprid, exposure in Sydney rock oysters

Ewere, Endurance E. ; Powell, Daniel LU ; Rudd, David ; Reichelt-Brushett, Amanda ; Mouatt, Peter ; Voelcker, Nicolas H. and Benkendorff, Kirsten (2019) In Chemosphere 230. p.1-13
Abstract

The broad utilisation of imidacloprid (IMI)in agriculture poses an increasing risk to aquatic organisms. However, the potential impacts on commercially important shellfish and chemical residues after exposure, are yet to be assessed. We investigated the levels of IMI in Sydney rock oyster (SRO)tissue during a three-day uptake and four-day depuration cycle using liquid chromatography-mass spectrometry. IMI was absorbed from the water, with significantly higher concentrations in the adductor muscles than the gills and digestive glands. Depuration was also fast with a significant drop in tissue concentrations after one day in clean water and complete elimination from all tissues except the digestive gland after four days. The distribution... (More)

The broad utilisation of imidacloprid (IMI)in agriculture poses an increasing risk to aquatic organisms. However, the potential impacts on commercially important shellfish and chemical residues after exposure, are yet to be assessed. We investigated the levels of IMI in Sydney rock oyster (SRO)tissue during a three-day uptake and four-day depuration cycle using liquid chromatography-mass spectrometry. IMI was absorbed from the water, with significantly higher concentrations in the adductor muscles than the gills and digestive glands. Depuration was also fast with a significant drop in tissue concentrations after one day in clean water and complete elimination from all tissues except the digestive gland after four days. The distribution of IMI in SRO after direct exposure using mass spectrometry imaging demonstrated uptake and spatially resolved metabolism to hydroxyl-IMI in the digestive gland and IMI-olefin in the gills. We assessed the effects of IMI on filtration rate (FR), acetylcholinesterase (AChE)activity in the gills, and gene expression profiles in the digestive gland using transcriptomics. Exposure to 2 mg/L IMI reduced the FR of oysters on the first day, while exposure to 0.5 and 1 mg/L reduced FR on day four. IMI reduced the gill AChE activity and altered the digestive gland gene expression profile. This study indicates that commercially farmed SRO can uptake IMI from the water, but negative impacts were only detected at concentrations higher than currently detected in estuarine environments and the chemical residues can be effectively eliminated using simple depuration in clean water.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Depuration, Mass spectrometry imaging, Neonicotinoids, Sublethal impacts, Sydney rock oysters, Transcriptomics
in
Chemosphere
volume
230
pages
13 pages
publisher
Elsevier
external identifiers
  • scopus:85065561253
  • pmid:31100675
ISSN
0045-6535
DOI
10.1016/j.chemosphere.2019.05.045
language
English
LU publication?
yes
id
55f202f4-71bd-463b-a1fa-637ba5f92c3b
date added to LUP
2019-05-22 09:00:09
date last changed
2021-06-20 06:35:01
@article{55f202f4-71bd-463b-a1fa-637ba5f92c3b,
  abstract     = {<p>The broad utilisation of imidacloprid (IMI)in agriculture poses an increasing risk to aquatic organisms. However, the potential impacts on commercially important shellfish and chemical residues after exposure, are yet to be assessed. We investigated the levels of IMI in Sydney rock oyster (SRO)tissue during a three-day uptake and four-day depuration cycle using liquid chromatography-mass spectrometry. IMI was absorbed from the water, with significantly higher concentrations in the adductor muscles than the gills and digestive glands. Depuration was also fast with a significant drop in tissue concentrations after one day in clean water and complete elimination from all tissues except the digestive gland after four days. The distribution of IMI in SRO after direct exposure using mass spectrometry imaging demonstrated uptake and spatially resolved metabolism to hydroxyl-IMI in the digestive gland and IMI-olefin in the gills. We assessed the effects of IMI on filtration rate (FR), acetylcholinesterase (AChE)activity in the gills, and gene expression profiles in the digestive gland using transcriptomics. Exposure to 2 mg/L IMI reduced the FR of oysters on the first day, while exposure to 0.5 and 1 mg/L reduced FR on day four. IMI reduced the gill AChE activity and altered the digestive gland gene expression profile. This study indicates that commercially farmed SRO can uptake IMI from the water, but negative impacts were only detected at concentrations higher than currently detected in estuarine environments and the chemical residues can be effectively eliminated using simple depuration in clean water.</p>},
  author       = {Ewere, Endurance E. and Powell, Daniel and Rudd, David and Reichelt-Brushett, Amanda and Mouatt, Peter and Voelcker, Nicolas H. and Benkendorff, Kirsten},
  issn         = {0045-6535},
  language     = {eng},
  pages        = {1--13},
  publisher    = {Elsevier},
  series       = {Chemosphere},
  title        = {Uptake, depuration and sublethal effects of the neonicotinoid, imidacloprid, exposure in Sydney rock oysters},
  url          = {http://dx.doi.org/10.1016/j.chemosphere.2019.05.045},
  doi          = {10.1016/j.chemosphere.2019.05.045},
  volume       = {230},
  year         = {2019},
}