Critical role for a stage-specific actin in male exflagellation of the malaria parasite
Deligianni, Elena ; Morgan, Rhiannon N ; Bertuccini, Lucia ; Kooij, Taco W A ; Laforge, Alice ; Nahar, Carolin ; Poulakakis, Nikos ; Schüler, Herwig LU
; Louis, Christos
and Matuschewski, Kai
, et al.
(2011)
In Cellular Microbiology
13(11).
p.30-1714
- Abstract
Male gametogenesis occurs directly after uptake of malaria parasites by the mosquito vector and leads to the release of eight nucleated flagellar gametes. Here, we report that one of the two parasite actin isoforms, named actin II, is essential for this process. Disruption of actin II in Plasmodium berghei resulted in viable asexual blood stages, but male gametogenesis was specifically inhibited. Upon activation, male gametocyte DNA was replicated normally and axonemes assembled, but egress from the host cell was inhibited, and axoneme motility abolished. The major actin isoform, actin I, displayed dual localization to the cytoplasm and the nucleus in male gametocytes. After activation actin I was found to be restricted to the... (More)
Male gametogenesis occurs directly after uptake of malaria parasites by the mosquito vector and leads to the release of eight nucleated flagellar gametes. Here, we report that one of the two parasite actin isoforms, named actin II, is essential for this process. Disruption of actin II in Plasmodium berghei resulted in viable asexual blood stages, but male gametogenesis was specifically inhibited. Upon activation, male gametocyte DNA was replicated normally and axonemes assembled, but egress from the host cell was inhibited, and axoneme motility abolished. The major actin isoform, actin I, displayed dual localization to the cytoplasm and the nucleus in male gametocytes. After activation actin I was found to be restricted to the cytoplasm. In actII(-) mutant parasites, this re-localization was abolished and actin I remained in both cellular compartments. These findings reveal vital and pleiotropic functions for the actin II isoform in male gametogenesis of the malaria parasite.
(Less)
- author
- Deligianni, Elena
; Morgan, Rhiannon N
; Bertuccini, Lucia
; Kooij, Taco W A
; Laforge, Alice
; Nahar, Carolin
; Poulakakis, Nikos
; Schüler, Herwig
LU
; Louis, Christos
and Matuschewski, Kai
, et al. (More)
- Deligianni, Elena
; Morgan, Rhiannon N
; Bertuccini, Lucia
; Kooij, Taco W A
; Laforge, Alice
; Nahar, Carolin
; Poulakakis, Nikos
; Schüler, Herwig
LU
; Louis, Christos
; Matuschewski, Kai
and Siden-Kiamos, Inga
(Less)
- publishing date
- 2011-11
- type
- Contribution to journal
- publication status
- published
- keywords
- Actins/genetics, Amino Acid Sequence, Animals, Cell Nucleus/chemistry, Cluster Analysis, Culicidae/parasitology, Cytoplasm/chemistry, Flagella/physiology, Gene Knockout Techniques, Microscopy, Electron, Transmission, Microscopy, Fluorescence, Molecular Sequence Data, Phylogeny, Plasmodium berghei/physiology, Sequence Homology, Amino Acid
- in
- Cellular Microbiology
- volume
- 13
- issue
- 11
- pages
- 17 pages
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:80054120241
- pmid:21790945
- ISSN
- 1462-5814
- DOI
- 10.1111/j.1462-5822.2011.01652.x
- language
- English
- LU publication?
- no
- additional info
- © 2011 Blackwell Publishing Ltd.
- id
- 561f3d50-c334-499b-a60a-19a89c17d2b7
- date added to LUP
- 2024-11-21 17:59:31
- date last changed
- 2025-01-03 07:45:37
@article{561f3d50-c334-499b-a60a-19a89c17d2b7,
abstract = {{<p>Male gametogenesis occurs directly after uptake of malaria parasites by the mosquito vector and leads to the release of eight nucleated flagellar gametes. Here, we report that one of the two parasite actin isoforms, named actin II, is essential for this process. Disruption of actin II in Plasmodium berghei resulted in viable asexual blood stages, but male gametogenesis was specifically inhibited. Upon activation, male gametocyte DNA was replicated normally and axonemes assembled, but egress from the host cell was inhibited, and axoneme motility abolished. The major actin isoform, actin I, displayed dual localization to the cytoplasm and the nucleus in male gametocytes. After activation actin I was found to be restricted to the cytoplasm. In actII(-) mutant parasites, this re-localization was abolished and actin I remained in both cellular compartments. These findings reveal vital and pleiotropic functions for the actin II isoform in male gametogenesis of the malaria parasite.</p>}},
author = {{Deligianni, Elena and Morgan, Rhiannon N and Bertuccini, Lucia and Kooij, Taco W A and Laforge, Alice and Nahar, Carolin and Poulakakis, Nikos and Schüler, Herwig and Louis, Christos and Matuschewski, Kai and Siden-Kiamos, Inga}},
issn = {{1462-5814}},
keywords = {{Actins/genetics; Amino Acid Sequence; Animals; Cell Nucleus/chemistry; Cluster Analysis; Culicidae/parasitology; Cytoplasm/chemistry; Flagella/physiology; Gene Knockout Techniques; Microscopy, Electron, Transmission; Microscopy, Fluorescence; Molecular Sequence Data; Phylogeny; Plasmodium berghei/physiology; Sequence Homology, Amino Acid}},
language = {{eng}},
number = {{11}},
pages = {{30--1714}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Cellular Microbiology}},
title = {{Critical role for a stage-specific actin in male exflagellation of the malaria parasite}},
url = {{http://dx.doi.org/10.1111/j.1462-5822.2011.01652.x}},
doi = {{10.1111/j.1462-5822.2011.01652.x}},
volume = {{13}},
year = {{2011}},
}