Advanced

The S1 split signal of photosystem II; a tyrosine-manganese coupled interaction

Cox, Nicholas ; Ho, Felix M. ; Pewnim, Naray ; Steffen, Ronald ; Smith, Paul J. ; Havelius, Kajsa G.V. LU ; Hughes, Joseph L. ; Debono, Lesley ; Styring, Stenbjörn LU and Krausz, Elmars , et al. (2009) In Biochimica et Biophysica Acta - Bioenergetics 1787(7). p.882-889
Abstract

Detailed optical and EPR analyses of states induced in dark-adapted PS II membranes by cryogenic illumination permit characterization and quantification of all pigment derived donors and acceptors, as well as optically silent (in the visible, near infrared) species which are EPR active. Near complete turnover formation of QA- is seen in all centers, but with variable efficiency, depending on the donor species. In minimally detergent-exposed PS II membranes, negligible (< 5%) oxidation of chlorophyll or carotenoid centers occurs for illumination temperatures 5-20 K. An optically silent electron donor to P680+ is observed with the same decay kinetics as the S1 split signal. Cryogenic donors... (More)

Detailed optical and EPR analyses of states induced in dark-adapted PS II membranes by cryogenic illumination permit characterization and quantification of all pigment derived donors and acceptors, as well as optically silent (in the visible, near infrared) species which are EPR active. Near complete turnover formation of QA- is seen in all centers, but with variable efficiency, depending on the donor species. In minimally detergent-exposed PS II membranes, negligible (< 5%) oxidation of chlorophyll or carotenoid centers occurs for illumination temperatures 5-20 K. An optically silent electron donor to P680+ is observed with the same decay kinetics as the S1 split signal. Cryogenic donors to P680+ seen are: (i) transient (t1/2 ∼ 150 s) tyrosine related species, including 'split signals' (∼ 15% total centers), (ii) reduced cytochrome b559 (∼ 30-50% centers), and (iii) an organic donor, possibly an amino acid side chain, (∼ 30% centers).

(Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Carotenoid, Chlorophyll, Cytochrome b, Exchange coupled interaction, Pheophytin, S split signal, Tyrosine
in
Biochimica et Biophysica Acta - Bioenergetics
volume
1787
issue
7
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:67649243700
  • pmid:19362068
ISSN
0005-2728
DOI
10.1016/j.bbabio.2009.03.023
language
English
LU publication?
no
id
563ef6d6-b632-4ac6-becd-59b198956f72
date added to LUP
2020-01-15 10:31:06
date last changed
2020-01-20 11:20:07
@article{563ef6d6-b632-4ac6-becd-59b198956f72,
  abstract     = {<p>Detailed optical and EPR analyses of states induced in dark-adapted PS II membranes by cryogenic illumination permit characterization and quantification of all pigment derived donors and acceptors, as well as optically silent (in the visible, near infrared) species which are EPR active. Near complete turnover formation of Q<sub>A</sub><sup>-</sup> is seen in all centers, but with variable efficiency, depending on the donor species. In minimally detergent-exposed PS II membranes, negligible (&lt; 5%) oxidation of chlorophyll or carotenoid centers occurs for illumination temperatures 5-20 K. An optically silent electron donor to P680<sup>+</sup> is observed with the same decay kinetics as the S<sub>1</sub> split signal. Cryogenic donors to P680<sup>+</sup> seen are: (i) transient (t<sub>1/2</sub> ∼ 150 s) tyrosine related species, including 'split signals' (∼ 15% total centers), (ii) reduced cytochrome b<sub>559</sub> (∼ 30-50% centers), and (iii) an organic donor, possibly an amino acid side chain, (∼ 30% centers).</p>},
  author       = {Cox, Nicholas and Ho, Felix M. and Pewnim, Naray and Steffen, Ronald and Smith, Paul J. and Havelius, Kajsa G.V. and Hughes, Joseph L. and Debono, Lesley and Styring, Stenbjörn and Krausz, Elmars and Pace, Ron J.},
  issn         = {0005-2728},
  language     = {eng},
  month        = {07},
  number       = {7},
  pages        = {882--889},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta - Bioenergetics},
  title        = {The S<sub>1</sub> split signal of photosystem II; a tyrosine-manganese coupled interaction},
  url          = {http://dx.doi.org/10.1016/j.bbabio.2009.03.023},
  doi          = {10.1016/j.bbabio.2009.03.023},
  volume       = {1787},
  year         = {2009},
}