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SLC20A1 Is Involved in Urinary Tract and Urorectal Development

Rieke, Johanna Magdalena ; Zhang, Rong ; Braun, Doreen ; Yilmaz, Öznur ; Japp, Anna S. ; Lopes, Filipa M. ; Pleschka, Michael ; Hilger, Alina C. ; Schneider, Sophia and Newman, William G. , et al. (2020) In Frontiers in cell and developmental biology 8.
Abstract

Previous studies in developing Xenopus and zebrafish reported that the phosphate transporter slc20a1a is expressed in pronephric kidneys. The recent identification of SLC20A1 as a monoallelic candidate gene for cloacal exstrophy further suggests its involvement in the urinary tract and urorectal development. However, little is known of the functional role of SLC20A1 in urinary tract development. Here, we investigated this using morpholino oligonucleotide knockdown of the zebrafish ortholog slc20a1a. This caused kidney cysts and malformations of the cloaca. Moreover, in morphants we demonstrated dysfunctional voiding and hindgut opening defects mimicking imperforate anus in human cloacal exstrophy. Furthermore, we performed... (More)

Previous studies in developing Xenopus and zebrafish reported that the phosphate transporter slc20a1a is expressed in pronephric kidneys. The recent identification of SLC20A1 as a monoallelic candidate gene for cloacal exstrophy further suggests its involvement in the urinary tract and urorectal development. However, little is known of the functional role of SLC20A1 in urinary tract development. Here, we investigated this using morpholino oligonucleotide knockdown of the zebrafish ortholog slc20a1a. This caused kidney cysts and malformations of the cloaca. Moreover, in morphants we demonstrated dysfunctional voiding and hindgut opening defects mimicking imperforate anus in human cloacal exstrophy. Furthermore, we performed immunohistochemistry of an unaffected 6-week-old human embryo and detected SLC20A1 in the urinary tract and the abdominal midline, structures implicated in the pathogenesis of cloacal exstrophy. Additionally, we resequenced SLC20A1 in 690 individuals with bladder exstrophy-epispadias complex (BEEC) including 84 individuals with cloacal exstrophy. We identified two additional monoallelic de novo variants. One was identified in a case-parent trio with classic bladder exstrophy, and one additional novel de novo variant was detected in an affected mother who transmitted this variant to her affected son. To study the potential cellular impact of SLC20A1 variants, we expressed them in HEK293 cells. Here, phosphate transport was not compromised, suggesting that it is not a disease mechanism. However, there was a tendency for lower levels of cleaved caspase-3, perhaps implicating apoptosis pathways in the disease. Our results suggest SLC20A1 is involved in urinary tract and urorectal development and implicate SLC20A1 as a disease-gene for BEEC.

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type
Contribution to journal
publication status
published
subject
keywords
bladder exstrophy-epispadias complex, CAKUT, cloacal malformation, functional genetics, kidney formation, SLC20A1, urinary tract development, zebrafish development
in
Frontiers in cell and developmental biology
volume
8
article number
567
publisher
Frontiers Media S. A.
external identifiers
  • scopus:85089850545
  • pmid:32850778
ISSN
2296-634X
DOI
10.3389/fcell.2020.00567
language
English
LU publication?
no
id
56407ec5-e925-4c8e-8cf0-b5b732afcbb9
date added to LUP
2020-09-08 12:11:37
date last changed
2021-01-13 07:56:10
@article{56407ec5-e925-4c8e-8cf0-b5b732afcbb9,
  abstract     = {<p>Previous studies in developing Xenopus and zebrafish reported that the phosphate transporter slc20a1a is expressed in pronephric kidneys. The recent identification of SLC20A1 as a monoallelic candidate gene for cloacal exstrophy further suggests its involvement in the urinary tract and urorectal development. However, little is known of the functional role of SLC20A1 in urinary tract development. Here, we investigated this using morpholino oligonucleotide knockdown of the zebrafish ortholog slc20a1a. This caused kidney cysts and malformations of the cloaca. Moreover, in morphants we demonstrated dysfunctional voiding and hindgut opening defects mimicking imperforate anus in human cloacal exstrophy. Furthermore, we performed immunohistochemistry of an unaffected 6-week-old human embryo and detected SLC20A1 in the urinary tract and the abdominal midline, structures implicated in the pathogenesis of cloacal exstrophy. Additionally, we resequenced SLC20A1 in 690 individuals with bladder exstrophy-epispadias complex (BEEC) including 84 individuals with cloacal exstrophy. We identified two additional monoallelic de novo variants. One was identified in a case-parent trio with classic bladder exstrophy, and one additional novel de novo variant was detected in an affected mother who transmitted this variant to her affected son. To study the potential cellular impact of SLC20A1 variants, we expressed them in HEK293 cells. Here, phosphate transport was not compromised, suggesting that it is not a disease mechanism. However, there was a tendency for lower levels of cleaved caspase-3, perhaps implicating apoptosis pathways in the disease. Our results suggest SLC20A1 is involved in urinary tract and urorectal development and implicate SLC20A1 as a disease-gene for BEEC.</p>},
  author       = {Rieke, Johanna Magdalena and Zhang, Rong and Braun, Doreen and Yilmaz, Öznur and Japp, Anna S. and Lopes, Filipa M. and Pleschka, Michael and Hilger, Alina C. and Schneider, Sophia and Newman, William G. and Beaman, Glenda M. and Nordenskjöld, Agneta and Ebert, Anne Karoline and Promm, Martin and Rösch, Wolfgang H. and Stein, Raimund and Hirsch, Karin and Schäfer, Frank Mattias and Schmiedeke, Eberhard and Boemers, Thomas M. and Lacher, Martin and Kluth, Dietrich and Gosemann, Jan Hendrik and Anderberg, Magnus and Barker, Gillian and Holmdahl, Gundela and Läckgren, Göran and Keene, David and Cervellione, Raimondo M. and Giorgio, Elisa and Di Grazia, Massimo and Feitz, Wouter F.J. and Marcelis, Carlo L.M. and Van Rooij, Iris A.L.M. and Bökenkamp, Arend and Beckers, Goedele M.A. and Keegan, Catherine E. and Sharma, Amit and Dakal, Tikam Chand and Wittler, Lars and Grote, Phillip and Zwink, Nadine and Jenetzky, Ekkehart and Brusco, Alfredo and Thiele, Holger and Ludwig, Michael and Schweizer, Ulrich and Woolf, Adrian S. and Odermatt, Benjamin and Reutter, Heiko},
  issn         = {2296-634X},
  language     = {eng},
  publisher    = {Frontiers Media S. A.},
  series       = {Frontiers in cell and developmental biology},
  title        = {SLC20A1 Is Involved in Urinary Tract and Urorectal Development},
  url          = {http://dx.doi.org/10.3389/fcell.2020.00567},
  doi          = {10.3389/fcell.2020.00567},
  volume       = {8},
  year         = {2020},
}