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Combined protein A imprinting and cryogelation for production of spherical affinity material

Aslıyüce, Sevgi ; Mattiasson, Bo LU and Denizli, Adil (2019) In Biomedical Chromatography 33(10).
Abstract

Cryogels have been demonstrated to be efficient when applied for protein isolation. Owing to their macroporous structure, cryogels can also be used for treating particle-containing material, e.g. cell homogenates. Another challenging development in protein purification technology is the use of molecularly imprinted polymers (MIPs). These MIPs are robust and can be used repeatedly. The paper presents a new technology that combine the formation of cryogel beads concomitantly with making imprints of a protein. Protein A was chosen as the print molecule which was also be the target in the purification step. The present paper describes a new method to produce protein-imprinted cryogel beads. The protein-imprinted material was characterized... (More)

Cryogels have been demonstrated to be efficient when applied for protein isolation. Owing to their macroporous structure, cryogels can also be used for treating particle-containing material, e.g. cell homogenates. Another challenging development in protein purification technology is the use of molecularly imprinted polymers (MIPs). These MIPs are robust and can be used repeatedly. The paper presents a new technology that combine the formation of cryogel beads concomitantly with making imprints of a protein. Protein A was chosen as the print molecule which was also be the target in the purification step. The present paper describes a new method to produce protein-imprinted cryogel beads. The protein-imprinted material was characterized and the separation properties were evaluated with regard to both the target protein and whole cells with target protein exposed on the cell surface. The maximum protein A adsorption was 18.1 mg/g of wet cryogel beads. The selectivity coefficient of protein A-imprinted cryogel beads for protein A was 5.44 and 12.56 times greater than for the Fc fragment of IgG and protein G, respectively.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cryogel beads, molecular imprinting technology, protein A, radical polymerization
in
Biomedical Chromatography
volume
33
issue
10
article number
e4605
publisher
John Wiley and Sons Inc.
external identifiers
  • scopus:85068522923
  • pmid:31140195
ISSN
0269-3879
DOI
10.1002/bmc.4605
language
English
LU publication?
yes
id
56621740-4e3b-4b6d-bf95-d06cb38b042f
date added to LUP
2019-07-17 15:05:50
date last changed
2020-08-02 06:51:58
@article{56621740-4e3b-4b6d-bf95-d06cb38b042f,
  abstract     = {<p>Cryogels have been demonstrated to be efficient when applied for protein isolation. Owing to their macroporous structure, cryogels can also be used for treating particle-containing material, e.g. cell homogenates. Another challenging development in protein purification technology is the use of molecularly imprinted polymers (MIPs). These MIPs are robust and can be used repeatedly. The paper presents a new technology that combine the formation of cryogel beads concomitantly with making imprints of a protein. Protein A was chosen as the print molecule which was also be the target in the purification step. The present paper describes a new method to produce protein-imprinted cryogel beads. The protein-imprinted material was characterized and the separation properties were evaluated with regard to both the target protein and whole cells with target protein exposed on the cell surface. The maximum protein A adsorption was 18.1 mg/g of wet cryogel beads. The selectivity coefficient of protein A-imprinted cryogel beads for protein A was 5.44 and 12.56 times greater than for the Fc fragment of IgG and protein G, respectively.</p>},
  author       = {Aslıyüce, Sevgi and Mattiasson, Bo and Denizli, Adil},
  issn         = {0269-3879},
  language     = {eng},
  number       = {10},
  publisher    = {John Wiley and Sons Inc.},
  series       = {Biomedical Chromatography},
  title        = {Combined protein A imprinting and cryogelation for production of spherical affinity material},
  url          = {http://dx.doi.org/10.1002/bmc.4605},
  doi          = {10.1002/bmc.4605},
  volume       = {33},
  year         = {2019},
}