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Isolation of interstitial fluid and demonstration of local proinflammatory cytokine production and increased absorptive gradient in chronic peritoneal dialysis

Rosengren, Bert-Inge LU ; Sagstad, Solfrid J. ; Karlsen, Tine V. and Wiig, Helge (2013) In American Journal of Physiology-Renal Physiology 304(2). p.198-206
Abstract
Rosengren BI, Sagstad SJ, Karlsen TV, Wiig H. Isolation of interstitial fluid and demonstration of local proinflammatory cytokine production and increased absorptive gradient in chronic peritoneal dialysis. Am J Physiol Renal Physiol 304: F198-F206, 2013. First published November 14, 2012; doi:10.1152/ajprenal.00293.2012.-In peritoneal dialysis (PD) patients, the frequent exposure to "unphysiological" dialysis fluids elicits a chronic state of a low-grade peritoneal inflammation leading to interstitial matrix remodeling and angiogenesis. Proinflammatory cytokines are important regulators involved in this inflammatory process that ultimately leads to dysfunction of the peritoneum as a dialysis membrane. We aimed to measure the local... (More)
Rosengren BI, Sagstad SJ, Karlsen TV, Wiig H. Isolation of interstitial fluid and demonstration of local proinflammatory cytokine production and increased absorptive gradient in chronic peritoneal dialysis. Am J Physiol Renal Physiol 304: F198-F206, 2013. First published November 14, 2012; doi:10.1152/ajprenal.00293.2012.-In peritoneal dialysis (PD) patients, the frequent exposure to "unphysiological" dialysis fluids elicits a chronic state of a low-grade peritoneal inflammation leading to interstitial matrix remodeling and angiogenesis. Proinflammatory cytokines are important regulators involved in this inflammatory process that ultimately leads to dysfunction of the peritoneum as a dialysis membrane. We aimed to measure the local concentrations of proinflammatory cytokines in the peritoneal interstitial fluid (IF). Furthermore, we wanted to assess how the driving forces for fluid and solute exchanges are affected in a remodeled interstitial matrix and thus measured the colloid osmotic pressure (COP) gradient in rats that were exposed to chronic PD. After 8 wk of peritoneal dialysis, IF from peritoneum was isolated using a centrifugation method, and was analyzed for cytokine content and COP along with plasma. For several of the proinflammatory cytokines there were gradients from IF to plasma, showing local production. For some cytokines, the concentration in IF was increased severalfold, whereas IL-18 was increased systemically due to PD. Furthermore, the presence of the catheter per se seemed to increase cytokine levels. COP in IF was significantly decreased in the PD group, while collagen and hyaluronan content was increased. Collectively, our data suggest that the increased levels of proinflammatory cytokines after PD may be an integral component of the development of fibrosis and angiogenesis commonly seen in PD patients, and the decreased COP in IF after chronic PD may shift the Starling equilibrium across peritoneal capillaries to an absorptive state. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
interstitial fluid, fluid reabsorption, colloid osmotic pressure, cytokines, inflammation
in
American Journal of Physiology-Renal Physiology
volume
304
issue
2
pages
198 - 206
publisher
American Physiological Society
external identifiers
  • wos:000313739600007
  • scopus:84872373367
  • pmid:23152294
ISSN
1522-1466
DOI
10.1152/ajprenal.00293.2012
language
English
LU publication?
yes
id
56c4400c-fc41-4e23-896b-ec877c351aad (old id 3595949)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23152294
date added to LUP
2016-04-01 10:55:44
date last changed
2022-03-20 01:15:54
@article{56c4400c-fc41-4e23-896b-ec877c351aad,
  abstract     = {{Rosengren BI, Sagstad SJ, Karlsen TV, Wiig H. Isolation of interstitial fluid and demonstration of local proinflammatory cytokine production and increased absorptive gradient in chronic peritoneal dialysis. Am J Physiol Renal Physiol 304: F198-F206, 2013. First published November 14, 2012; doi:10.1152/ajprenal.00293.2012.-In peritoneal dialysis (PD) patients, the frequent exposure to "unphysiological" dialysis fluids elicits a chronic state of a low-grade peritoneal inflammation leading to interstitial matrix remodeling and angiogenesis. Proinflammatory cytokines are important regulators involved in this inflammatory process that ultimately leads to dysfunction of the peritoneum as a dialysis membrane. We aimed to measure the local concentrations of proinflammatory cytokines in the peritoneal interstitial fluid (IF). Furthermore, we wanted to assess how the driving forces for fluid and solute exchanges are affected in a remodeled interstitial matrix and thus measured the colloid osmotic pressure (COP) gradient in rats that were exposed to chronic PD. After 8 wk of peritoneal dialysis, IF from peritoneum was isolated using a centrifugation method, and was analyzed for cytokine content and COP along with plasma. For several of the proinflammatory cytokines there were gradients from IF to plasma, showing local production. For some cytokines, the concentration in IF was increased severalfold, whereas IL-18 was increased systemically due to PD. Furthermore, the presence of the catheter per se seemed to increase cytokine levels. COP in IF was significantly decreased in the PD group, while collagen and hyaluronan content was increased. Collectively, our data suggest that the increased levels of proinflammatory cytokines after PD may be an integral component of the development of fibrosis and angiogenesis commonly seen in PD patients, and the decreased COP in IF after chronic PD may shift the Starling equilibrium across peritoneal capillaries to an absorptive state.}},
  author       = {{Rosengren, Bert-Inge and Sagstad, Solfrid J. and Karlsen, Tine V. and Wiig, Helge}},
  issn         = {{1522-1466}},
  keywords     = {{interstitial fluid; fluid reabsorption; colloid osmotic pressure; cytokines; inflammation}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{198--206}},
  publisher    = {{American Physiological Society}},
  series       = {{American Journal of Physiology-Renal Physiology}},
  title        = {{Isolation of interstitial fluid and demonstration of local proinflammatory cytokine production and increased absorptive gradient in chronic peritoneal dialysis}},
  url          = {{http://dx.doi.org/10.1152/ajprenal.00293.2012}},
  doi          = {{10.1152/ajprenal.00293.2012}},
  volume       = {{304}},
  year         = {{2013}},
}