An ELISA for apolipoprotein M reveals a strong correlation to total cholesterol in human plasma
(2007) In Journal of Lipid Research 48(8). p.1772-1780- Abstract
- Apolipoprotein M (apoM) is a 188 amino acid, 25 kDa protein belonging to the lipocalin protein superfamily. Although predominantly associated with high density lipoprotein, apoM is found in all major lipoprotein classes. To facilitate clinical studies of apoM, we have developed a sandwich ELISA for the measurement of apoM in human plasma. This method has been used to investigate normal apoM variation and to establish reference values for healthy individuals through the measurement of 598 samples from the Nordic Reference Interval Project Bio-bank and Database ( NOBIDA) biobank. For women 18-49 years old, the reference interval for apoM was 0.58-1.18 mu mol/l, whereas for women 501 years and for men, the reference range was 0.61-1.30 mu... (More)
- Apolipoprotein M (apoM) is a 188 amino acid, 25 kDa protein belonging to the lipocalin protein superfamily. Although predominantly associated with high density lipoprotein, apoM is found in all major lipoprotein classes. To facilitate clinical studies of apoM, we have developed a sandwich ELISA for the measurement of apoM in human plasma. This method has been used to investigate normal apoM variation and to establish reference values for healthy individuals through the measurement of 598 samples from the Nordic Reference Interval Project Bio-bank and Database ( NOBIDA) biobank. For women 18-49 years old, the reference interval for apoM was 0.58-1.18 mu mol/l, whereas for women 501 years and for men, the reference range was 0.61-1.30 mu mol/l. Correlation studies of apoM with 26 common clinical chemical analytes from the NOBIDA database revealed a marked positive correlation with plasma total cholesterol (r = 0.52) and LDL and HDL cholesterol ( r = 0.43 and 0.36, respectively). There was no statistically significant correlation with HDL/total cholesterol ratio or body mass index. In conclusion, a sandwich ELISA for the measurement of apoM in human plasma shows that apoM concentration is strongly correlated to total cholesterol in healthy individuals. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/691677
- author
- Axler, Olof LU ; Ahnström, Josefin LU and Dahlbäck, Björn LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- enzyme-linked immunosorbent assay, interval project, lipocalin, nordic reference, and database, reference interval project bio-bank, nordic, biobank, reference range, reference interval, high density lipoprotein, low density lipoprotein
- in
- Journal of Lipid Research
- volume
- 48
- issue
- 8
- pages
- 1772 - 1780
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- wos:000248044200012
- scopus:34548176847
- ISSN
- 1539-7262
- DOI
- 10.1194/jlr.M700113-JLR200
- language
- English
- LU publication?
- yes
- id
- 56f33d01-60ff-428d-8336-680273c041b8 (old id 691677)
- date added to LUP
- 2016-04-01 12:13:15
- date last changed
- 2022-03-05 20:38:31
@article{56f33d01-60ff-428d-8336-680273c041b8, abstract = {{Apolipoprotein M (apoM) is a 188 amino acid, 25 kDa protein belonging to the lipocalin protein superfamily. Although predominantly associated with high density lipoprotein, apoM is found in all major lipoprotein classes. To facilitate clinical studies of apoM, we have developed a sandwich ELISA for the measurement of apoM in human plasma. This method has been used to investigate normal apoM variation and to establish reference values for healthy individuals through the measurement of 598 samples from the Nordic Reference Interval Project Bio-bank and Database ( NOBIDA) biobank. For women 18-49 years old, the reference interval for apoM was 0.58-1.18 mu mol/l, whereas for women 501 years and for men, the reference range was 0.61-1.30 mu mol/l. Correlation studies of apoM with 26 common clinical chemical analytes from the NOBIDA database revealed a marked positive correlation with plasma total cholesterol (r = 0.52) and LDL and HDL cholesterol ( r = 0.43 and 0.36, respectively). There was no statistically significant correlation with HDL/total cholesterol ratio or body mass index. In conclusion, a sandwich ELISA for the measurement of apoM in human plasma shows that apoM concentration is strongly correlated to total cholesterol in healthy individuals.}}, author = {{Axler, Olof and Ahnström, Josefin and Dahlbäck, Björn}}, issn = {{1539-7262}}, keywords = {{enzyme-linked immunosorbent assay; interval project; lipocalin; nordic reference; and database; reference interval project bio-bank; nordic; biobank; reference range; reference interval; high density lipoprotein; low density lipoprotein}}, language = {{eng}}, number = {{8}}, pages = {{1772--1780}}, publisher = {{American Society for Biochemistry and Molecular Biology}}, series = {{Journal of Lipid Research}}, title = {{An ELISA for apolipoprotein M reveals a strong correlation to total cholesterol in human plasma}}, url = {{http://dx.doi.org/10.1194/jlr.M700113-JLR200}}, doi = {{10.1194/jlr.M700113-JLR200}}, volume = {{48}}, year = {{2007}}, }