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Role and significance of plasma citrulline in the early phase after small bowel transplantation in pigs

Nadalin, Silvio ; Biglarnia, Ali Reza LU orcid ; Testa, Giuliano ; Koppara, Tobias R ; Schaffer, Randolph ; Johnson, Cecilia ; Toetsch, Martin ; Broelsch, Christoph E and Malagó, Massimo (2007) In Transplant international : official journal of the European Society for Organ Transplantation 20(5). p.31-425
Abstract

A reliable serological marker of acute cellular rejection (ACR) after small bowel transplantation (SBTx) is still missing. Plasma citrulline level (PCL) reflects the functional integrity of intestinal mucosa which is partially lost during ACR. The aim of our study was to investigate the role of PCL as marker of ACR after SBTx. Eighteen German landrace pigs were used and divided into three groups. Group 1 (G1), autologous SBTx (n = 4) as control; group 2 (G2), allogeneic SBTx without immunosuppression (IS) (n = 7) and group 3 (G3), allogeneic SBTx with IS (n = 7). IS consisted of tacrolimus and steroids without induction treatment. Observation period was 14 days. Mucosal biopsies were obtained intraoperatively and daily using a... (More)

A reliable serological marker of acute cellular rejection (ACR) after small bowel transplantation (SBTx) is still missing. Plasma citrulline level (PCL) reflects the functional integrity of intestinal mucosa which is partially lost during ACR. The aim of our study was to investigate the role of PCL as marker of ACR after SBTx. Eighteen German landrace pigs were used and divided into three groups. Group 1 (G1), autologous SBTx (n = 4) as control; group 2 (G2), allogeneic SBTx without immunosuppression (IS) (n = 7) and group 3 (G3), allogeneic SBTx with IS (n = 7). IS consisted of tacrolimus and steroids without induction treatment. Observation period was 14 days. Mucosal biopsies were obtained intraoperatively and daily using a Thiry-Vella loop. ACR was differentiated into indeterminate, mild, moderate and severe using a standardized grading schema. PCL was measured daily. An ACR onset occurred generally from postoperative day 4 both in G2 and G3 as mild form and developed differently in the two groups: moderate to severe in G2 and indeterminate to mild in G3. A significant decline of PCL occurred only in cases of moderate and severe ACR, but not in cases of indeterminate and mild ACR. The PCL failed as a marker in the early diagnosis of ACR and became reliable only when advanced mucosal damage was present.

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author
; ; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Biomarkers/blood, Citrulline/blood, Female, Graft Rejection/blood, Intestine, Small/pathology, Organ Transplantation/adverse effects, Reperfusion Injury/pathology, Swine
in
Transplant international : official journal of the European Society for Organ Transplantation
volume
20
issue
5
pages
31 - 425
publisher
Springer
external identifiers
  • scopus:34047222987
  • pmid:17313447
ISSN
0934-0874
DOI
10.1111/j.1432-2277.2007.00460.x
language
English
LU publication?
no
id
57083a51-4cf5-41ce-99d0-6167ef2111e9
date added to LUP
2025-12-17 14:24:20
date last changed
2026-01-01 05:21:03
@article{57083a51-4cf5-41ce-99d0-6167ef2111e9,
  abstract     = {{<p>A reliable serological marker of acute cellular rejection (ACR) after small bowel transplantation (SBTx) is still missing. Plasma citrulline level (PCL) reflects the functional integrity of intestinal mucosa which is partially lost during ACR. The aim of our study was to investigate the role of PCL as marker of ACR after SBTx. Eighteen German landrace pigs were used and divided into three groups. Group 1 (G1), autologous SBTx (n = 4) as control; group 2 (G2), allogeneic SBTx without immunosuppression (IS) (n = 7) and group 3 (G3), allogeneic SBTx with IS (n = 7). IS consisted of tacrolimus and steroids without induction treatment. Observation period was 14 days. Mucosal biopsies were obtained intraoperatively and daily using a Thiry-Vella loop. ACR was differentiated into indeterminate, mild, moderate and severe using a standardized grading schema. PCL was measured daily. An ACR onset occurred generally from postoperative day 4 both in G2 and G3 as mild form and developed differently in the two groups: moderate to severe in G2 and indeterminate to mild in G3. A significant decline of PCL occurred only in cases of moderate and severe ACR, but not in cases of indeterminate and mild ACR. The PCL failed as a marker in the early diagnosis of ACR and became reliable only when advanced mucosal damage was present.</p>}},
  author       = {{Nadalin, Silvio and Biglarnia, Ali Reza and Testa, Giuliano and Koppara, Tobias R and Schaffer, Randolph and Johnson, Cecilia and Toetsch, Martin and Broelsch, Christoph E and Malagó, Massimo}},
  issn         = {{0934-0874}},
  keywords     = {{Animals; Biomarkers/blood; Citrulline/blood; Female; Graft Rejection/blood; Intestine, Small/pathology; Organ Transplantation/adverse effects; Reperfusion Injury/pathology; Swine}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{31--425}},
  publisher    = {{Springer}},
  series       = {{Transplant international : official journal of the European Society for Organ Transplantation}},
  title        = {{Role and significance of plasma citrulline in the early phase after small bowel transplantation in pigs}},
  url          = {{http://dx.doi.org/10.1111/j.1432-2277.2007.00460.x}},
  doi          = {{10.1111/j.1432-2277.2007.00460.x}},
  volume       = {{20}},
  year         = {{2007}},
}