Dihydroorotate dehydrogenase mRNA and protein expression analysis in normal and drug-resistant cells
(2004) In Nucleosides, Nucleotides & Nucleic Acids 23(8-9). p.1281-1285- Abstract
To follow the expression of the fourth enzyme of pyrimidine de novo synthesis dihydroorotate dehydrogenase (DHODH) in cells and tissues, we studied the DHODH mRNA expression by means of RT-PCR in rat tissues. Rabbit polyclonal anti-DHODH immunoglobulins were applied for immunochemical quantification of the enzyme protein by Western blotting. In mouse B-lymphocytes, which were adapted to tolerate up to a 50-fold concentration of the DHODH inhibitor leflunomide, a 20 fold protein overexpression was measured. Southern blotting indicated DHODH gene amplification.
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- author
- Löffler, Monika ; Klein, A ; Hayek-Ouassini, M ; Knecht, W LU and Konrad, L
- publishing date
- 2004-10
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Blotting, Southern, Blotting, Western, Cell Line, DNA/chemistry, Drug Resistance, Humans, Immunoglobulins/metabolism, Immunohistochemistry, Inhibitory Concentration 50, Isoxazoles/pharmacology, Leflunomide, Lymphocytes/metabolism, Male, Membranes/metabolism, Mice, Mitochondria/metabolism, Oxidoreductases Acting on CH-CH Group Donors/metabolism, Pyrimidines/metabolism, RNA, Messenger/metabolism, Rats, Recombinant Proteins/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Testis, Time Factors
- in
- Nucleosides, Nucleotides & Nucleic Acids
- volume
- 23
- issue
- 8-9
- pages
- 1281 - 1285
- publisher
- Taylor & Francis
- external identifiers
-
- scopus:10344247697
- pmid:15571246
- ISSN
- 1525-7770
- DOI
- 10.1081/NCN-200027547
- language
- English
- LU publication?
- no
- id
- 57bf4249-c6a1-425d-9fd7-621272d45f42
- date added to LUP
- 2020-07-22 14:17:17
- date last changed
- 2024-05-01 15:08:14
@article{57bf4249-c6a1-425d-9fd7-621272d45f42, abstract = {{<p>To follow the expression of the fourth enzyme of pyrimidine de novo synthesis dihydroorotate dehydrogenase (DHODH) in cells and tissues, we studied the DHODH mRNA expression by means of RT-PCR in rat tissues. Rabbit polyclonal anti-DHODH immunoglobulins were applied for immunochemical quantification of the enzyme protein by Western blotting. In mouse B-lymphocytes, which were adapted to tolerate up to a 50-fold concentration of the DHODH inhibitor leflunomide, a 20 fold protein overexpression was measured. Southern blotting indicated DHODH gene amplification.</p>}}, author = {{Löffler, Monika and Klein, A and Hayek-Ouassini, M and Knecht, W and Konrad, L}}, issn = {{1525-7770}}, keywords = {{Animals; Blotting, Southern; Blotting, Western; Cell Line; DNA/chemistry; Drug Resistance; Humans; Immunoglobulins/metabolism; Immunohistochemistry; Inhibitory Concentration 50; Isoxazoles/pharmacology; Leflunomide; Lymphocytes/metabolism; Male; Membranes/metabolism; Mice; Mitochondria/metabolism; Oxidoreductases Acting on CH-CH Group Donors/metabolism; Pyrimidines/metabolism; RNA, Messenger/metabolism; Rats; Recombinant Proteins/metabolism; Reverse Transcriptase Polymerase Chain Reaction; Testis; Time Factors}}, language = {{eng}}, number = {{8-9}}, pages = {{1281--1285}}, publisher = {{Taylor & Francis}}, series = {{Nucleosides, Nucleotides & Nucleic Acids}}, title = {{Dihydroorotate dehydrogenase mRNA and protein expression analysis in normal and drug-resistant cells}}, url = {{http://dx.doi.org/10.1081/NCN-200027547}}, doi = {{10.1081/NCN-200027547}}, volume = {{23}}, year = {{2004}}, }