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Deep immune cell phenotyping and induced immune cell responses at admission stratified by BMI in patients hospitalized with COVID-19 : An observational multicenter cohort pilot study

Sejdic, Adin LU ; Hartling, Hans Jakob ; Gitz Holler, Jon ; Klingen Gjærde, Lars ; Matovu Dungu, Arnold ; Engel Møller, Maria Elizabeth ; Svanberg Teglgaard, Rebecca ; Utoft Niemann, Carsten Utoft ; Brooks, Patrick Terrence and Mogensen, Trine H , et al. (2024) In Clinical Immunology 267.
Abstract

INTRODUCTION: Overweight and obesity are linked to increased hospitalization and mortality in COVID-19 patients. This study aimed to characterize induced immune responses and deep immune cell profiles stratified by BMI in hospitalized COVID-19 patients.

METHODS AND RESULTS: This observational multicenter cohort pilot study included 122 adult patients with PCR-confirmed COVID-19 in Denmark, stratified by BMI (normal weight, overweight, obese). Inflammation was assessed using TruCulture® and immune cell profiles by flow cytometry with a customized antibody panel (DuraClone®). Patients with obesity had a more pro-inflammatory phenotype with increased TNF-α, IL-8, IL-17, and IL-10 levels post-T cell stimulation, and altered B cell... (More)

INTRODUCTION: Overweight and obesity are linked to increased hospitalization and mortality in COVID-19 patients. This study aimed to characterize induced immune responses and deep immune cell profiles stratified by BMI in hospitalized COVID-19 patients.

METHODS AND RESULTS: This observational multicenter cohort pilot study included 122 adult patients with PCR-confirmed COVID-19 in Denmark, stratified by BMI (normal weight, overweight, obese). Inflammation was assessed using TruCulture® and immune cell profiles by flow cytometry with a customized antibody panel (DuraClone®). Patients with obesity had a more pro-inflammatory phenotype with increased TNF-α, IL-8, IL-17, and IL-10 levels post-T cell stimulation, and altered B cell profiles. Patients with obesity showed higher concentrations of naïve, transitional, and non-isotype switched memory B cells, and plasmablasts compared to normal weight patients and healthy controls.

CONCLUSIONS: Obesity in hospitalized COVID-19 patients may correlate with elevated pro-inflammatory cytokines, anti-inflammatory IL-10, and increased B cell subset activation, highlighting the need for further studies.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
Humans, COVID-19/immunology, Male, Pilot Projects, Female, Body Mass Index, Middle Aged, Obesity/immunology, Aged, SARS-CoV-2/immunology, Cytokines/immunology, Cohort Studies, Adult, Hospitalization, Denmark, Immunophenotyping, B-Lymphocytes/immunology, Overweight/immunology
in
Clinical Immunology
volume
267
article number
110336
publisher
Academic Press
external identifiers
  • pmid:39117044
  • scopus:85201002798
ISSN
1521-6616
DOI
10.1016/j.clim.2024.110336
language
English
LU publication?
no
additional info
Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.
id
57d4661f-cc3f-4c24-b7b2-11a037dc7f9c
date added to LUP
2024-10-14 09:17:03
date last changed
2025-07-09 02:27:43
@article{57d4661f-cc3f-4c24-b7b2-11a037dc7f9c,
  abstract     = {{<p>INTRODUCTION: Overweight and obesity are linked to increased hospitalization and mortality in COVID-19 patients. This study aimed to characterize induced immune responses and deep immune cell profiles stratified by BMI in hospitalized COVID-19 patients.</p><p>METHODS AND RESULTS: This observational multicenter cohort pilot study included 122 adult patients with PCR-confirmed COVID-19 in Denmark, stratified by BMI (normal weight, overweight, obese). Inflammation was assessed using TruCulture® and immune cell profiles by flow cytometry with a customized antibody panel (DuraClone®). Patients with obesity had a more pro-inflammatory phenotype with increased TNF-α, IL-8, IL-17, and IL-10 levels post-T cell stimulation, and altered B cell profiles. Patients with obesity showed higher concentrations of naïve, transitional, and non-isotype switched memory B cells, and plasmablasts compared to normal weight patients and healthy controls.</p><p>CONCLUSIONS: Obesity in hospitalized COVID-19 patients may correlate with elevated pro-inflammatory cytokines, anti-inflammatory IL-10, and increased B cell subset activation, highlighting the need for further studies.</p>}},
  author       = {{Sejdic, Adin and Hartling, Hans Jakob and Gitz Holler, Jon and Klingen Gjærde, Lars and Matovu Dungu, Arnold and Engel Møller, Maria Elizabeth and Svanberg Teglgaard, Rebecca and Utoft Niemann, Carsten Utoft and Brooks, Patrick Terrence and Mogensen, Trine H and Weis, Nina and Podlekareva, Daria and Baum Jørgensen, Marie Louise and Ortved Gang, Anne and Stampe Hersby, Ditte and Hald, Annemette and Dam Nielsen, Susanne and Lebech, Anne-Mette and Helleberg, Marie and Lundgren, Jens and Træholt Franck, Kristina and Fischer, Thea K and Barrella Harboe, Zitta and Marquart, Hanne Vibeke and Rye Ostrowski, Sisse and Lindegaard, Birgitte}},
  issn         = {{1521-6616}},
  keywords     = {{Humans; COVID-19/immunology; Male; Pilot Projects; Female; Body Mass Index; Middle Aged; Obesity/immunology; Aged; SARS-CoV-2/immunology; Cytokines/immunology; Cohort Studies; Adult; Hospitalization; Denmark; Immunophenotyping; B-Lymphocytes/immunology; Overweight/immunology}},
  language     = {{eng}},
  publisher    = {{Academic Press}},
  series       = {{Clinical Immunology}},
  title        = {{Deep immune cell phenotyping and induced immune cell responses at admission stratified by BMI in patients hospitalized with COVID-19 : An observational multicenter cohort pilot study}},
  url          = {{http://dx.doi.org/10.1016/j.clim.2024.110336}},
  doi          = {{10.1016/j.clim.2024.110336}},
  volume       = {{267}},
  year         = {{2024}},
}