Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis
(2018) In Molecular Biotechnology 60(8). p.595-600- Abstract
Leishmaniasis is one of the most important neglected tropical diseases, with a broad spectrum of clinical manifestations. Among the clinical manifestations of the disease, cutaneous leishmaniasis, caused by species of Leishmania braziliensis, presents wide distribution in Brazil. In this work, we performed the cloning, expression, and purification of the enzyme superoxide dismutase of Leishmania braziliensis (LbSOD-B2) considered a promising target for the search of new compounds against leishmaniasis. In vitro assays based on pyrogallol oxidation showed that LbSOD-B2 is most active around pH 8 and hydrogen peroxide is a LbSOD-B2 inhibitor at low millimolar range (IC50 = 1 mM).
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/57dc9905-bf13-44a1-8f30-64bf4335e660
- author
- Brito, Camila C.B. ; Maluf, Fernando V. ; de Lima, Gustavo M.A. LU ; Guido, Rafael V.C. and Castilho, Marcelo S.
- organization
- publishing date
- 2018-06-27
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Heterologous expression, Kinetic assay, Leishmania braziliensis, Superoxide dismutase
- in
- Molecular Biotechnology
- volume
- 60
- issue
- 8
- pages
- 595 - 600
- publisher
- Humana Press
- external identifiers
-
- pmid:29951736
- scopus:85049114270
- ISSN
- 1073-6085
- DOI
- 10.1007/s12033-018-0095-2
- language
- English
- LU publication?
- yes
- id
- 57dc9905-bf13-44a1-8f30-64bf4335e660
- date added to LUP
- 2018-07-09 11:53:47
- date last changed
- 2024-06-24 16:50:59
@article{57dc9905-bf13-44a1-8f30-64bf4335e660, abstract = {{<p>Leishmaniasis is one of the most important neglected tropical diseases, with a broad spectrum of clinical manifestations. Among the clinical manifestations of the disease, cutaneous leishmaniasis, caused by species of Leishmania braziliensis, presents wide distribution in Brazil. In this work, we performed the cloning, expression, and purification of the enzyme superoxide dismutase of Leishmania braziliensis (LbSOD-B2) considered a promising target for the search of new compounds against leishmaniasis. In vitro assays based on pyrogallol oxidation showed that LbSOD-B2 is most active around pH 8 and hydrogen peroxide is a LbSOD-B2 inhibitor at low millimolar range (IC<sub>50</sub> = 1 mM).</p>}}, author = {{Brito, Camila C.B. and Maluf, Fernando V. and de Lima, Gustavo M.A. and Guido, Rafael V.C. and Castilho, Marcelo S.}}, issn = {{1073-6085}}, keywords = {{Heterologous expression; Kinetic assay; Leishmania braziliensis; Superoxide dismutase}}, language = {{eng}}, month = {{06}}, number = {{8}}, pages = {{595--600}}, publisher = {{Humana Press}}, series = {{Molecular Biotechnology}}, title = {{Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis}}, url = {{http://dx.doi.org/10.1007/s12033-018-0095-2}}, doi = {{10.1007/s12033-018-0095-2}}, volume = {{60}}, year = {{2018}}, }