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Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis

Brito, Camila C.B. ; Maluf, Fernando V. ; de Lima, Gustavo M.A. LU orcid ; Guido, Rafael V.C. and Castilho, Marcelo S. (2018) In Molecular Biotechnology 60(8). p.595-600
Abstract

Leishmaniasis is one of the most important neglected tropical diseases, with a broad spectrum of clinical manifestations. Among the clinical manifestations of the disease, cutaneous leishmaniasis, caused by species of Leishmania braziliensis, presents wide distribution in Brazil. In this work, we performed the cloning, expression, and purification of the enzyme superoxide dismutase of Leishmania braziliensis (LbSOD-B2) considered a promising target for the search of new compounds against leishmaniasis. In vitro assays based on pyrogallol oxidation showed that LbSOD-B2 is most active around pH 8 and hydrogen peroxide is a LbSOD-B2 inhibitor at low millimolar range (IC50 = 1 mM).

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Heterologous expression, Kinetic assay, Leishmania braziliensis, Superoxide dismutase
in
Molecular Biotechnology
volume
60
issue
8
pages
595 - 600
publisher
Humana Press
external identifiers
  • scopus:85049114270
  • pmid:29951736
ISSN
1073-6085
DOI
10.1007/s12033-018-0095-2
language
English
LU publication?
yes
id
57dc9905-bf13-44a1-8f30-64bf4335e660
date added to LUP
2018-07-09 11:53:47
date last changed
2024-01-29 18:34:07
@article{57dc9905-bf13-44a1-8f30-64bf4335e660,
  abstract     = {{<p>Leishmaniasis is one of the most important neglected tropical diseases, with a broad spectrum of clinical manifestations. Among the clinical manifestations of the disease, cutaneous leishmaniasis, caused by species of Leishmania braziliensis, presents wide distribution in Brazil. In this work, we performed the cloning, expression, and purification of the enzyme superoxide dismutase of Leishmania braziliensis (LbSOD-B2) considered a promising target for the search of new compounds against leishmaniasis. In vitro assays based on pyrogallol oxidation showed that LbSOD-B2 is most active around pH 8 and hydrogen peroxide is a LbSOD-B2 inhibitor at low millimolar range (IC<sub>50</sub> = 1 mM).</p>}},
  author       = {{Brito, Camila C.B. and Maluf, Fernando V. and de Lima, Gustavo M.A. and Guido, Rafael V.C. and Castilho, Marcelo S.}},
  issn         = {{1073-6085}},
  keywords     = {{Heterologous expression; Kinetic assay; Leishmania braziliensis; Superoxide dismutase}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{8}},
  pages        = {{595--600}},
  publisher    = {{Humana Press}},
  series       = {{Molecular Biotechnology}},
  title        = {{Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis}},
  url          = {{http://dx.doi.org/10.1007/s12033-018-0095-2}},
  doi          = {{10.1007/s12033-018-0095-2}},
  volume       = {{60}},
  year         = {{2018}},
}