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Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis

Brito, Camila C.B.; Maluf, Fernando V.; de Lima, Gustavo M.A. LU ; Guido, Rafael V.C. and Castilho, Marcelo S. (2018) In Molecular Biotechnology p.1-6
Abstract

Leishmaniasis is one of the most important neglected tropical diseases, with a broad spectrum of clinical manifestations. Among the clinical manifestations of the disease, cutaneous leishmaniasis, caused by species of Leishmania braziliensis, presents wide distribution in Brazil. In this work, we performed the cloning, expression, and purification of the enzyme superoxide dismutase of Leishmania braziliensis (LbSOD-B2) considered a promising target for the search of new compounds against leishmaniasis. In vitro assays based on pyrogallol oxidation showed that LbSOD-B2 is most active around pH 8 and hydrogen peroxide is a LbSOD-B2 inhibitor at low millimolar range (IC50 = 1 mM).

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
in press
subject
keywords
Heterologous expression, Kinetic assay, Leishmania braziliensis, Superoxide dismutase
in
Molecular Biotechnology
pages
6 pages
publisher
Humana Press
external identifiers
  • scopus:85049114270
ISSN
1073-6085
DOI
10.1007/s12033-018-0095-2
language
English
LU publication?
yes
id
57dc9905-bf13-44a1-8f30-64bf4335e660
date added to LUP
2018-07-09 11:53:47
date last changed
2018-07-10 03:00:05
@article{57dc9905-bf13-44a1-8f30-64bf4335e660,
  abstract     = {<p>Leishmaniasis is one of the most important neglected tropical diseases, with a broad spectrum of clinical manifestations. Among the clinical manifestations of the disease, cutaneous leishmaniasis, caused by species of Leishmania braziliensis, presents wide distribution in Brazil. In this work, we performed the cloning, expression, and purification of the enzyme superoxide dismutase of Leishmania braziliensis (LbSOD-B2) considered a promising target for the search of new compounds against leishmaniasis. In vitro assays based on pyrogallol oxidation showed that LbSOD-B2 is most active around pH 8 and hydrogen peroxide is a LbSOD-B2 inhibitor at low millimolar range (IC<sub>50</sub> = 1 mM).</p>},
  author       = {Brito, Camila C.B. and Maluf, Fernando V. and de Lima, Gustavo M.A. and Guido, Rafael V.C. and Castilho, Marcelo S.},
  issn         = {1073-6085},
  keyword      = {Heterologous expression,Kinetic assay,Leishmania braziliensis,Superoxide dismutase},
  language     = {eng},
  month        = {06},
  pages        = {1--6},
  publisher    = {Humana Press},
  series       = {Molecular Biotechnology},
  title        = {Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis},
  url          = {http://dx.doi.org/10.1007/s12033-018-0095-2},
  year         = {2018},
}