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Mutual enhancement of the current density and the coulombic efficiency for a bioanode by entrapping bi-enzymes with Os-complex modified electrodeposition paints

Shao, Minling; Zafar, MNadeem LU ; Sygmund, Christoph; Guschin, Dmitrii A.; Ludwig, Roland; Peterbauer, Clemens K.; Schuhmann, Wolfgang and Gorton, Lo LU (2013) In Biosensors & Bioelectronics 40(1). p.308-314
Abstract
A bioanode with high current density and coulombic efficiency was developed by co-immobilization of pyranose dehydrogenase from Agaricus meleagris (AmPDH) with the dehydrogenase domain of cellobiose dehydrogenase from Corynascus thermophiles (recDHCtCDH) expressed recombinantly in Escherichia coli. The two enzymes were entrapped in Os-complex modified electrodeposition polymers (Os-EDPs) with specifically adapted redox potential by means of chemical co-deposition. AmPDH oxidizes glucose at both the C2 and C3 positions whereas recDHCtCDH oxidizes glucose only at the Cl position. Electrochemical measurements reveal that maximally 6 electrons can be harvested from one glucose molecule at the two-enzyme anode via a cascade reaction, as AmPDH... (More)
A bioanode with high current density and coulombic efficiency was developed by co-immobilization of pyranose dehydrogenase from Agaricus meleagris (AmPDH) with the dehydrogenase domain of cellobiose dehydrogenase from Corynascus thermophiles (recDHCtCDH) expressed recombinantly in Escherichia coli. The two enzymes were entrapped in Os-complex modified electrodeposition polymers (Os-EDPs) with specifically adapted redox potential by means of chemical co-deposition. AmPDH oxidizes glucose at both the C2 and C3 positions whereas recDHCtCDH oxidizes glucose only at the Cl position. Electrochemical measurements reveal that maximally 6 electrons can be harvested from one glucose molecule at the two-enzyme anode via a cascade reaction, as AmPDH oxidizes the products formed from of the recDHCtCDH catalyzed substrate oxidation and vice versa. Furthermore, a significant increase in current density can be obtained by combining AmPDH and recDHCtCDH in a single modified electrode. We propose the use of this bioanode in biofuel cells with increased current density and coulombic efficiency. (C) 2012 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cellobiose dehydrogenase (CDH), Coulombic efficiency, Pyranose, dehydrogenase (PDH), Os-complex modified polymer, Redox polymer, Electron transfer
in
Biosensors & Bioelectronics
volume
40
issue
1
pages
308 - 314
publisher
Elsevier
external identifiers
  • wos:000312825800049
  • scopus:84868640042
ISSN
1873-4235
DOI
10.1016/j.bios.2012.07.069
language
English
LU publication?
yes
id
5836b8f4-ebbe-4311-92fb-fa8340999d29 (old id 3476908)
date added to LUP
2013-02-26 08:01:40
date last changed
2019-06-19 02:17:53
@article{5836b8f4-ebbe-4311-92fb-fa8340999d29,
  abstract     = {A bioanode with high current density and coulombic efficiency was developed by co-immobilization of pyranose dehydrogenase from Agaricus meleagris (AmPDH) with the dehydrogenase domain of cellobiose dehydrogenase from Corynascus thermophiles (recDHCtCDH) expressed recombinantly in Escherichia coli. The two enzymes were entrapped in Os-complex modified electrodeposition polymers (Os-EDPs) with specifically adapted redox potential by means of chemical co-deposition. AmPDH oxidizes glucose at both the C2 and C3 positions whereas recDHCtCDH oxidizes glucose only at the Cl position. Electrochemical measurements reveal that maximally 6 electrons can be harvested from one glucose molecule at the two-enzyme anode via a cascade reaction, as AmPDH oxidizes the products formed from of the recDHCtCDH catalyzed substrate oxidation and vice versa. Furthermore, a significant increase in current density can be obtained by combining AmPDH and recDHCtCDH in a single modified electrode. We propose the use of this bioanode in biofuel cells with increased current density and coulombic efficiency. (C) 2012 Elsevier B.V. All rights reserved.},
  author       = {Shao, Minling and Zafar, MNadeem and Sygmund, Christoph and Guschin, Dmitrii A. and Ludwig, Roland and Peterbauer, Clemens K. and Schuhmann, Wolfgang and Gorton, Lo},
  issn         = {1873-4235},
  keyword      = {Cellobiose dehydrogenase (CDH),Coulombic efficiency,Pyranose,dehydrogenase (PDH),Os-complex modified polymer,Redox polymer,Electron transfer},
  language     = {eng},
  number       = {1},
  pages        = {308--314},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Mutual enhancement of the current density and the coulombic efficiency for a bioanode by entrapping bi-enzymes with Os-complex modified electrodeposition paints},
  url          = {http://dx.doi.org/10.1016/j.bios.2012.07.069},
  volume       = {40},
  year         = {2013},
}