Regulation of IRS-1, insulin signaling and glucose uptake by miR-143/145 in vascular smooth muscle cells
(2020) In Biochemical and Biophysical Research Communications 529(1). p.119-125- Abstract
Regulation of insulin signaling by microRNAs in smooth muscle cells may contribute to diabetic vascular disease. The two smooth muscle enriched miRNAs miR-143 and miR-145 have been reported to target mediators of insulin signaling in non-smooth muscle cells. In this study, we aimed to determine the importance of this regulation in vascular smooth muscle cells, where expression of miR-143/145 is much higher than in other cell types. Smooth muscle cells deficient of the miR-143/145 cluster were used, as well as smooth muscle cells transfected with mimics/inhibitors for either miR-143 or miR-145. We found that deletion of miR-143/145 in smooth muscle results in a dramatic upregulation IRS-1 expression and insulin signaling, and an... (More)
Regulation of insulin signaling by microRNAs in smooth muscle cells may contribute to diabetic vascular disease. The two smooth muscle enriched miRNAs miR-143 and miR-145 have been reported to target mediators of insulin signaling in non-smooth muscle cells. In this study, we aimed to determine the importance of this regulation in vascular smooth muscle cells, where expression of miR-143/145 is much higher than in other cell types. Smooth muscle cells deficient of the miR-143/145 cluster were used, as well as smooth muscle cells transfected with mimics/inhibitors for either miR-143 or miR-145. We found that deletion of miR-143/145 in smooth muscle results in a dramatic upregulation IRS-1 expression and insulin signaling, and an increased insulin-induced glucose uptake. Furthermore, specific modulation of either miR-145 or miR-143 expression regulated specific targets (IRS-1, ORP8 and the IGF-1 receptor) in the insulin signaling pathway. Consequently, transient inhibition or overexpression of either miR-143 or miR-145 was sufficient to regulate insulin signaling in smooth muscle cells. In conclusion, the results of this study support an important role for both miR-143 and miR-145 in the regulation of insulin signaling and glucose uptake in vascular smooth muscle cells.
(Less)
- author
- Lan, Susan LU and Albinsson, Sebastian LU
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Glucose, Insulin, IRS-1, microRNA, miR-145, Vascular smooth muscle
- in
- Biochemical and Biophysical Research Communications
- volume
- 529
- issue
- 1
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:85085972549
- pmid:32560812
- ISSN
- 0006-291X
- DOI
- 10.1016/j.bbrc.2020.05.148
- language
- English
- LU publication?
- yes
- id
- 583c5f71-1942-4363-a93e-cbeb2c308004
- date added to LUP
- 2020-07-01 12:30:29
- date last changed
- 2024-05-30 18:24:22
@article{583c5f71-1942-4363-a93e-cbeb2c308004,
abstract = {{<p>Regulation of insulin signaling by microRNAs in smooth muscle cells may contribute to diabetic vascular disease. The two smooth muscle enriched miRNAs miR-143 and miR-145 have been reported to target mediators of insulin signaling in non-smooth muscle cells. In this study, we aimed to determine the importance of this regulation in vascular smooth muscle cells, where expression of miR-143/145 is much higher than in other cell types. Smooth muscle cells deficient of the miR-143/145 cluster were used, as well as smooth muscle cells transfected with mimics/inhibitors for either miR-143 or miR-145. We found that deletion of miR-143/145 in smooth muscle results in a dramatic upregulation IRS-1 expression and insulin signaling, and an increased insulin-induced glucose uptake. Furthermore, specific modulation of either miR-145 or miR-143 expression regulated specific targets (IRS-1, ORP8 and the IGF-1 receptor) in the insulin signaling pathway. Consequently, transient inhibition or overexpression of either miR-143 or miR-145 was sufficient to regulate insulin signaling in smooth muscle cells. In conclusion, the results of this study support an important role for both miR-143 and miR-145 in the regulation of insulin signaling and glucose uptake in vascular smooth muscle cells.</p>}},
author = {{Lan, Susan and Albinsson, Sebastian}},
issn = {{0006-291X}},
keywords = {{Glucose; Insulin; IRS-1; microRNA; miR-145; Vascular smooth muscle}},
language = {{eng}},
number = {{1}},
pages = {{119--125}},
publisher = {{Elsevier}},
series = {{Biochemical and Biophysical Research Communications}},
title = {{Regulation of IRS-1, insulin signaling and glucose uptake by miR-143/145 in vascular smooth muscle cells}},
url = {{http://dx.doi.org/10.1016/j.bbrc.2020.05.148}},
doi = {{10.1016/j.bbrc.2020.05.148}},
volume = {{529}},
year = {{2020}},
}