Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels.
(2005) In Genome Research 15(10). p.1388-1392- Abstract
- The transcriptional machinery in individual cells is controlled by a relatively small number of molecules, which may result in stochastic behavior in gene activity. Because of technical limitations in current collection and recording methods, most gene expression measurements are carried out on populations of cells and therefore reflect average mRNA levels. The variability of the transcript levels between different cells remains undefined, although it may have profound effects on cellular activities. Here we have measured gene expression levels of the five genes ActB, Ins1, Ins2, Abcc8, and Kcnj11 in individual cells from mouse pancreatic islets. Whereas Ins1 and Ins2 expression show a strong cell-cell correlation, this is not the case for... (More)
- The transcriptional machinery in individual cells is controlled by a relatively small number of molecules, which may result in stochastic behavior in gene activity. Because of technical limitations in current collection and recording methods, most gene expression measurements are carried out on populations of cells and therefore reflect average mRNA levels. The variability of the transcript levels between different cells remains undefined, although it may have profound effects on cellular activities. Here we have measured gene expression levels of the five genes ActB, Ins1, Ins2, Abcc8, and Kcnj11 in individual cells from mouse pancreatic islets. Whereas Ins1 and Ins2 expression show a strong cell-cell correlation, this is not the case for the other genes. We further found that the transcript levels of the different genes are lognormally distributed. Hence, the geometric mean of expression levels provides a better estimate of gene activity of the typical cell than does the arithmetic mean measured on a cell population. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/144843
- author
- Bengtsson, Martin LU ; Stahlberg, Anders ; Rorsman, Patrik LU and Kubista, Mikael
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Genome Research
- volume
- 15
- issue
- 10
- pages
- 1388 - 1392
- publisher
- Cold Spring Harbor Laboratory Press (CSHL)
- external identifiers
-
- wos:000232436800009
- pmid:16204192
- scopus:25844433114
- ISSN
- 1549-5469
- DOI
- 10.1101/gr.3820805
- language
- English
- LU publication?
- yes
- id
- 5986f78b-4f7d-4a2c-b9e8-02c578af41ed (old id 144843)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16204192&dopt=Abstract
- date added to LUP
- 2016-04-01 15:28:26
- date last changed
- 2022-04-22 07:52:59
@article{5986f78b-4f7d-4a2c-b9e8-02c578af41ed, abstract = {{The transcriptional machinery in individual cells is controlled by a relatively small number of molecules, which may result in stochastic behavior in gene activity. Because of technical limitations in current collection and recording methods, most gene expression measurements are carried out on populations of cells and therefore reflect average mRNA levels. The variability of the transcript levels between different cells remains undefined, although it may have profound effects on cellular activities. Here we have measured gene expression levels of the five genes ActB, Ins1, Ins2, Abcc8, and Kcnj11 in individual cells from mouse pancreatic islets. Whereas Ins1 and Ins2 expression show a strong cell-cell correlation, this is not the case for the other genes. We further found that the transcript levels of the different genes are lognormally distributed. Hence, the geometric mean of expression levels provides a better estimate of gene activity of the typical cell than does the arithmetic mean measured on a cell population.}}, author = {{Bengtsson, Martin and Stahlberg, Anders and Rorsman, Patrik and Kubista, Mikael}}, issn = {{1549-5469}}, language = {{eng}}, number = {{10}}, pages = {{1388--1392}}, publisher = {{Cold Spring Harbor Laboratory Press (CSHL)}}, series = {{Genome Research}}, title = {{Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels.}}, url = {{http://dx.doi.org/10.1101/gr.3820805}}, doi = {{10.1101/gr.3820805}}, volume = {{15}}, year = {{2005}}, }