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Assessment of Polluted Soil Remediation Using Bacterial Community Tolerance to Heavy Metals as an Indicator

Campillo-Cora, Claudia ; Soto-Gómez, Diego LU ; Arias-Estévez, Manuel and Fernández-Calviño, David LU (2022) In agronomy 12(10).
Abstract

The assessment of remediation on metal-polluted soils is usually focused on total and/or bioavailable metal content. However, these chemical variables do not provide direct information about reductions in heavy metals pressure on soil microorganisms. We propose the use of bacterial communities to evaluate the efficiency of three remediation techniques: crushed mussel shell (CMS) and pine bark (PB) as soil amendments and EDTA-washing. A soil sample was polluted with different doses of Cu, Ni, and Zn (separately). After 30 days of incubation, the remediation techniques were applied, and bacterial community tolerance to heavy metals determined. If bacterial communities develop tolerance, it is an indicator that the metal is exerting... (More)

The assessment of remediation on metal-polluted soils is usually focused on total and/or bioavailable metal content. However, these chemical variables do not provide direct information about reductions in heavy metals pressure on soil microorganisms. We propose the use of bacterial communities to evaluate the efficiency of three remediation techniques: crushed mussel shell (CMS) and pine bark (PB) as soil amendments and EDTA-washing. A soil sample was polluted with different doses of Cu, Ni, and Zn (separately). After 30 days of incubation, the remediation techniques were applied, and bacterial community tolerance to heavy metals determined. If bacterial communities develop tolerance, it is an indicator that the metal is exerting toxicity on them. Soil bacterial communities developed tolerance to Cu, Ni, and Zn in response to metal additions. After remediation, bacterial communities showed decreases in bacterial community tolerance to Cu, Ni, and Zn for all remediation techniques. For Cu and Ni, soil EDTA-washing showed the greatest reduction of bacterial community tolerance to Cu and Ni, respectively, while for Zn the soil amendment with PB was the most effective remediation technique. Thus, bacterial community tolerance to heavy metals successfully detect differences in the effectiveness of the three remediation techniques.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bacterial growth, bio-sorbents, metal pollution, Pollution-Induced Community Tolerance (PICT), risk assessment, soil bioremediation, soil washing
in
agronomy
volume
12
issue
10
article number
2280
publisher
MDPI AG
external identifiers
  • scopus:85140439021
ISSN
2073-4395
DOI
10.3390/agronomy12102280
language
English
LU publication?
yes
id
5998a974-bc60-4c77-bf33-2b719f938bd6
date added to LUP
2022-12-14 13:15:37
date last changed
2022-12-14 13:15:37
@article{5998a974-bc60-4c77-bf33-2b719f938bd6,
  abstract     = {{<p>The assessment of remediation on metal-polluted soils is usually focused on total and/or bioavailable metal content. However, these chemical variables do not provide direct information about reductions in heavy metals pressure on soil microorganisms. We propose the use of bacterial communities to evaluate the efficiency of three remediation techniques: crushed mussel shell (CMS) and pine bark (PB) as soil amendments and EDTA-washing. A soil sample was polluted with different doses of Cu, Ni, and Zn (separately). After 30 days of incubation, the remediation techniques were applied, and bacterial community tolerance to heavy metals determined. If bacterial communities develop tolerance, it is an indicator that the metal is exerting toxicity on them. Soil bacterial communities developed tolerance to Cu, Ni, and Zn in response to metal additions. After remediation, bacterial communities showed decreases in bacterial community tolerance to Cu, Ni, and Zn for all remediation techniques. For Cu and Ni, soil EDTA-washing showed the greatest reduction of bacterial community tolerance to Cu and Ni, respectively, while for Zn the soil amendment with PB was the most effective remediation technique. Thus, bacterial community tolerance to heavy metals successfully detect differences in the effectiveness of the three remediation techniques.</p>}},
  author       = {{Campillo-Cora, Claudia and Soto-Gómez, Diego and Arias-Estévez, Manuel and Fernández-Calviño, David}},
  issn         = {{2073-4395}},
  keywords     = {{bacterial growth; bio-sorbents; metal pollution; Pollution-Induced Community Tolerance (PICT); risk assessment; soil bioremediation; soil washing}},
  language     = {{eng}},
  number       = {{10}},
  publisher    = {{MDPI AG}},
  series       = {{agronomy}},
  title        = {{Assessment of Polluted Soil Remediation Using Bacterial Community Tolerance to Heavy Metals as an Indicator}},
  url          = {{http://dx.doi.org/10.3390/agronomy12102280}},
  doi          = {{10.3390/agronomy12102280}},
  volume       = {{12}},
  year         = {{2022}},
}