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Taurine effects on bisphenol a-induced oxidative stress in the mouse testicular mitochondria and sperm motility

Rezaee-Tazangi, Fatemeh ; Zeidooni, Leila ; Rafiee, Zeinab LU ; Fakhredini, Fereshtesadat ; Kalantari, Heybatollah ; Alidadi, Hadis and Khorsandi, Layasadat (2020) In Jornal Brasileiro de Reproducao Assistida 24(4). p.428-435
Abstract

Objectives: This study was performed to investigate the protective effects of taurine (2-aminoethanesulfonic acid, TAU) on oxidative stress in the isolated mouse testicular mitochondria, mitochondrial membrane potential (MMP), viability and motility of the exposed sperms to the BPA. Methods: We treated epididymal spermatozoa obtained from mice and isolated mouse testicular mitochondria with BPA (0.8 mmol/mL) and various doses of TAU (5, 10, 30 and 50 µmol/L). We used the MTT assay and Rhodamine 123 uptake to assess sperm viability and MMP. We assessed the oxidative stress through measuring ROS (reactive oxygen species), MDA (malondialdehyde), GSH (glutathione), and SOD (super-oxide dismutase) levels in the testicular mitochondrial... (More)

Objectives: This study was performed to investigate the protective effects of taurine (2-aminoethanesulfonic acid, TAU) on oxidative stress in the isolated mouse testicular mitochondria, mitochondrial membrane potential (MMP), viability and motility of the exposed sperms to the BPA. Methods: We treated epididymal spermatozoa obtained from mice and isolated mouse testicular mitochondria with BPA (0.8 mmol/mL) and various doses of TAU (5, 10, 30 and 50 µmol/L). We used the MTT assay and Rhodamine 123 uptake to assess sperm viability and MMP. We assessed the oxidative stress through measuring ROS (reactive oxygen species), MDA (malondialdehyde), GSH (glutathione), and SOD (super-oxide dismutase) levels in the testicular mitochondrial tissue. Results: BPA significantly elevated ROS, MDA and MMP levels, and markedly reduced SOD and GSH levels in the isolated mitochondria. BPA also considerably impaired spermatozoa viability and motility. Pretreatment with 30 and 50 µmol/L of TAU could considerably suppressed mitochondrial oxidative stress, enhanced MMP, and improved sperm motility and viability. Conclusion: TAU may attenuate the BPA-induced mitochondrial toxicity and impaired sperm motility via decreasing oxidative stress.

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bisphenol A, Mitochondria, Oxidative stress, Sperm motility, Taurine
in
Jornal Brasileiro de Reproducao Assistida
volume
24
issue
4
pages
428 - 435
publisher
Sociedade Brasileira de Reproducao Assistida
external identifiers
  • pmid:32401462
  • scopus:85089409329
ISSN
1517-5693
DOI
10.5935/1518-0557.20200017
language
English
LU publication?
no
additional info
Funding Information: Student Research committee of Ahvaz Jundishapur University funded this work (funding number: 97s26). Publisher Copyright: © 2020, Sociedade Brasileira de Reproducao Assistida. All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
id
59a1d2a0-0fc5-4e78-93a3-a614f2422b86
date added to LUP
2021-09-23 20:39:20
date last changed
2024-06-15 16:46:46
@article{59a1d2a0-0fc5-4e78-93a3-a614f2422b86,
  abstract     = {{<p>Objectives: This study was performed to investigate the protective effects of taurine (2-aminoethanesulfonic acid, TAU) on oxidative stress in the isolated mouse testicular mitochondria, mitochondrial membrane potential (MMP), viability and motility of the exposed sperms to the BPA. Methods: We treated epididymal spermatozoa obtained from mice and isolated mouse testicular mitochondria with BPA (0.8 mmol/mL) and various doses of TAU (5, 10, 30 and 50 µmol/L). We used the MTT assay and Rhodamine 123 uptake to assess sperm viability and MMP. We assessed the oxidative stress through measuring ROS (reactive oxygen species), MDA (malondialdehyde), GSH (glutathione), and SOD (super-oxide dismutase) levels in the testicular mitochondrial tissue. Results: BPA significantly elevated ROS, MDA and MMP levels, and markedly reduced SOD and GSH levels in the isolated mitochondria. BPA also considerably impaired spermatozoa viability and motility. Pretreatment with 30 and 50 µmol/L of TAU could considerably suppressed mitochondrial oxidative stress, enhanced MMP, and improved sperm motility and viability. Conclusion: TAU may attenuate the BPA-induced mitochondrial toxicity and impaired sperm motility via decreasing oxidative stress.</p>}},
  author       = {{Rezaee-Tazangi, Fatemeh and Zeidooni, Leila and Rafiee, Zeinab and Fakhredini, Fereshtesadat and Kalantari, Heybatollah and Alidadi, Hadis and Khorsandi, Layasadat}},
  issn         = {{1517-5693}},
  keywords     = {{Bisphenol A; Mitochondria; Oxidative stress; Sperm motility; Taurine}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{428--435}},
  publisher    = {{Sociedade Brasileira de Reproducao Assistida}},
  series       = {{Jornal Brasileiro de Reproducao Assistida}},
  title        = {{Taurine effects on bisphenol a-induced oxidative stress in the mouse testicular mitochondria and sperm motility}},
  url          = {{http://dx.doi.org/10.5935/1518-0557.20200017}},
  doi          = {{10.5935/1518-0557.20200017}},
  volume       = {{24}},
  year         = {{2020}},
}