Taurine effects on bisphenol a-induced oxidative stress in the mouse testicular mitochondria and sperm motility
(2020) In Jornal Brasileiro de Reproducao Assistida 24(4). p.428-435- Abstract
Objectives: This study was performed to investigate the protective effects of taurine (2-aminoethanesulfonic acid, TAU) on oxidative stress in the isolated mouse testicular mitochondria, mitochondrial membrane potential (MMP), viability and motility of the exposed sperms to the BPA. Methods: We treated epididymal spermatozoa obtained from mice and isolated mouse testicular mitochondria with BPA (0.8 mmol/mL) and various doses of TAU (5, 10, 30 and 50 µmol/L). We used the MTT assay and Rhodamine 123 uptake to assess sperm viability and MMP. We assessed the oxidative stress through measuring ROS (reactive oxygen species), MDA (malondialdehyde), GSH (glutathione), and SOD (super-oxide dismutase) levels in the testicular mitochondrial... (More)
Objectives: This study was performed to investigate the protective effects of taurine (2-aminoethanesulfonic acid, TAU) on oxidative stress in the isolated mouse testicular mitochondria, mitochondrial membrane potential (MMP), viability and motility of the exposed sperms to the BPA. Methods: We treated epididymal spermatozoa obtained from mice and isolated mouse testicular mitochondria with BPA (0.8 mmol/mL) and various doses of TAU (5, 10, 30 and 50 µmol/L). We used the MTT assay and Rhodamine 123 uptake to assess sperm viability and MMP. We assessed the oxidative stress through measuring ROS (reactive oxygen species), MDA (malondialdehyde), GSH (glutathione), and SOD (super-oxide dismutase) levels in the testicular mitochondrial tissue. Results: BPA significantly elevated ROS, MDA and MMP levels, and markedly reduced SOD and GSH levels in the isolated mitochondria. BPA also considerably impaired spermatozoa viability and motility. Pretreatment with 30 and 50 µmol/L of TAU could considerably suppressed mitochondrial oxidative stress, enhanced MMP, and improved sperm motility and viability. Conclusion: TAU may attenuate the BPA-induced mitochondrial toxicity and impaired sperm motility via decreasing oxidative stress.
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- author
- Rezaee-Tazangi, Fatemeh ; Zeidooni, Leila ; Rafiee, Zeinab LU ; Fakhredini, Fereshtesadat ; Kalantari, Heybatollah ; Alidadi, Hadis and Khorsandi, Layasadat
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bisphenol A, Mitochondria, Oxidative stress, Sperm motility, Taurine
- in
- Jornal Brasileiro de Reproducao Assistida
- volume
- 24
- issue
- 4
- pages
- 428 - 435
- publisher
- Sociedade Brasileira de Reproducao Assistida
- external identifiers
-
- scopus:85089409329
- pmid:32401462
- ISSN
- 1517-5693
- DOI
- 10.5935/1518-0557.20200017
- language
- English
- LU publication?
- no
- additional info
- Funding Information: Student Research committee of Ahvaz Jundishapur University funded this work (funding number: 97s26). Publisher Copyright: © 2020, Sociedade Brasileira de Reproducao Assistida. All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
- id
- 59a1d2a0-0fc5-4e78-93a3-a614f2422b86
- date added to LUP
- 2021-09-23 20:39:20
- date last changed
- 2024-08-24 23:39:21
@article{59a1d2a0-0fc5-4e78-93a3-a614f2422b86, abstract = {{<p>Objectives: This study was performed to investigate the protective effects of taurine (2-aminoethanesulfonic acid, TAU) on oxidative stress in the isolated mouse testicular mitochondria, mitochondrial membrane potential (MMP), viability and motility of the exposed sperms to the BPA. Methods: We treated epididymal spermatozoa obtained from mice and isolated mouse testicular mitochondria with BPA (0.8 mmol/mL) and various doses of TAU (5, 10, 30 and 50 µmol/L). We used the MTT assay and Rhodamine 123 uptake to assess sperm viability and MMP. We assessed the oxidative stress through measuring ROS (reactive oxygen species), MDA (malondialdehyde), GSH (glutathione), and SOD (super-oxide dismutase) levels in the testicular mitochondrial tissue. Results: BPA significantly elevated ROS, MDA and MMP levels, and markedly reduced SOD and GSH levels in the isolated mitochondria. BPA also considerably impaired spermatozoa viability and motility. Pretreatment with 30 and 50 µmol/L of TAU could considerably suppressed mitochondrial oxidative stress, enhanced MMP, and improved sperm motility and viability. Conclusion: TAU may attenuate the BPA-induced mitochondrial toxicity and impaired sperm motility via decreasing oxidative stress.</p>}}, author = {{Rezaee-Tazangi, Fatemeh and Zeidooni, Leila and Rafiee, Zeinab and Fakhredini, Fereshtesadat and Kalantari, Heybatollah and Alidadi, Hadis and Khorsandi, Layasadat}}, issn = {{1517-5693}}, keywords = {{Bisphenol A; Mitochondria; Oxidative stress; Sperm motility; Taurine}}, language = {{eng}}, number = {{4}}, pages = {{428--435}}, publisher = {{Sociedade Brasileira de Reproducao Assistida}}, series = {{Jornal Brasileiro de Reproducao Assistida}}, title = {{Taurine effects on bisphenol a-induced oxidative stress in the mouse testicular mitochondria and sperm motility}}, url = {{http://dx.doi.org/10.5935/1518-0557.20200017}}, doi = {{10.5935/1518-0557.20200017}}, volume = {{24}}, year = {{2020}}, }