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Identifying miRNA targets using AGO-RIPseq

Petri, Rebecca LU and Jakobsson, Johan LU (2018) In Methods in Molecular Biology 1720. p.131-140
Abstract

microRNAs (miRNA) are small, noncoding RNAs that bind to messenger RNAs (mRNAs) and regulate their activity. They are, therefore, important posttranscriptional regulators. In recent years it has become clear that miRNAs regulate large genetic networks, rather than single genes, and that one gene can be targeted by several miRNAs. To understand the role of miRNAs in cells or tissues, it is therefore important to analyze the targetome of miRNAs. Here, we present a technique called Argonaute-RNA Immunoprecipitation (AGO-RIP) which takes advantages of the fact that miRNAs and their targets are directly bound by the Argonaute protein family. With this approach quantitative, genome-wide analysis of miRNA targets is possible. In this chapter... (More)

microRNAs (miRNA) are small, noncoding RNAs that bind to messenger RNAs (mRNAs) and regulate their activity. They are, therefore, important posttranscriptional regulators. In recent years it has become clear that miRNAs regulate large genetic networks, rather than single genes, and that one gene can be targeted by several miRNAs. To understand the role of miRNAs in cells or tissues, it is therefore important to analyze the targetome of miRNAs. Here, we present a technique called Argonaute-RNA Immunoprecipitation (AGO-RIP) which takes advantages of the fact that miRNAs and their targets are directly bound by the Argonaute protein family. With this approach quantitative, genome-wide analysis of miRNA targets is possible. In this chapter we describe the RIP-methodology and provide advice for RNA sequencing and bioinformatic analyses.

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Argonaute, Immunoprecipitation, microRNAs, Small and total RNA sequencing, Targetome
in
Methods in Molecular Biology
volume
1720
pages
10 pages
publisher
Humana Press
external identifiers
  • scopus:85038231578
ISSN
1064-3745
DOI
language
English
LU publication?
yes
id
59b472d7-53f3-4ae2-a59c-7e3343b07287
date added to LUP
2018-01-03 07:43:42
date last changed
2018-05-29 10:50:55
@inbook{59b472d7-53f3-4ae2-a59c-7e3343b07287,
  abstract     = {<p>microRNAs (miRNA) are small, noncoding RNAs that bind to messenger RNAs (mRNAs) and regulate their activity. They are, therefore, important posttranscriptional regulators. In recent years it has become clear that miRNAs regulate large genetic networks, rather than single genes, and that one gene can be targeted by several miRNAs. To understand the role of miRNAs in cells or tissues, it is therefore important to analyze the targetome of miRNAs. Here, we present a technique called Argonaute-RNA Immunoprecipitation (AGO-RIP) which takes advantages of the fact that miRNAs and their targets are directly bound by the Argonaute protein family. With this approach quantitative, genome-wide analysis of miRNA targets is possible. In this chapter we describe the RIP-methodology and provide advice for RNA sequencing and bioinformatic analyses.</p>},
  author       = {Petri, Rebecca and Jakobsson, Johan},
  issn         = {1064-3745},
  keyword      = {Argonaute,Immunoprecipitation,microRNAs,Small and total RNA sequencing,Targetome},
  language     = {eng},
  pages        = {131--140},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Identifying miRNA targets using AGO-RIPseq},
  url          = {http://dx.doi.org/},
  volume       = {1720},
  year         = {2018},
}