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Membrane-bound FRET probe visualizes MMP12 activity in pulmonary inflammation

Cobos-Correa, Amanda ; Trojanek, Johanna B. ; Diemer, Stefanie LU ; Mall, Marcus A. and Schultz, Carsten (2009) In Nature Chemical Biology 5(9). p.628-630
Abstract

MMP12 is a metalloproteinase implicated in inflammation. To monitor its activity, we synthesized a membrane-targeted reporter (LaRee1) based on Foerster resonance energy transfer (FRET). Unlike existing sensors, LaRee1 detects MMP12 activity by loss of FRET plus internalization of the lipidated fragment. In bronchoalveolar lavages from a mouse model of pulmonary inflammation, LaRee1 detected MMP12 activity at the surface of activated macrophages. LaRee1 may become a powerful tool for monitoring lung disease.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
in
Nature Chemical Biology
volume
5
issue
9
pages
628 - 630
publisher
Nature Publishing Group
external identifiers
  • scopus:69249231952
  • pmid:19648933
ISSN
1552-4450
DOI
10.1038/nchembio.196
language
English
LU publication?
no
id
59e1b482-dd6e-49ed-adab-af5635812b4a
date added to LUP
2024-04-29 16:30:59
date last changed
2024-04-29 16:32:04
@article{59e1b482-dd6e-49ed-adab-af5635812b4a,
  abstract     = {{<p>MMP12 is a metalloproteinase implicated in inflammation. To monitor its activity, we synthesized a membrane-targeted reporter (LaRee1) based on Foerster resonance energy transfer (FRET). Unlike existing sensors, LaRee1 detects MMP12 activity by loss of FRET plus internalization of the lipidated fragment. In bronchoalveolar lavages from a mouse model of pulmonary inflammation, LaRee1 detected MMP12 activity at the surface of activated macrophages. LaRee1 may become a powerful tool for monitoring lung disease.</p>}},
  author       = {{Cobos-Correa, Amanda and Trojanek, Johanna B. and Diemer, Stefanie and Mall, Marcus A. and Schultz, Carsten}},
  issn         = {{1552-4450}},
  language     = {{eng}},
  number       = {{9}},
  pages        = {{628--630}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Nature Chemical Biology}},
  title        = {{Membrane-bound FRET probe visualizes MMP12 activity in pulmonary inflammation}},
  url          = {{http://dx.doi.org/10.1038/nchembio.196}},
  doi          = {{10.1038/nchembio.196}},
  volume       = {{5}},
  year         = {{2009}},
}