Membrane-bound FRET probe visualizes MMP12 activity in pulmonary inflammation
(2009) In Nature Chemical Biology 5(9). p.628-630- Abstract
MMP12 is a metalloproteinase implicated in inflammation. To monitor its activity, we synthesized a membrane-targeted reporter (LaRee1) based on Foerster resonance energy transfer (FRET). Unlike existing sensors, LaRee1 detects MMP12 activity by loss of FRET plus internalization of the lipidated fragment. In bronchoalveolar lavages from a mouse model of pulmonary inflammation, LaRee1 detected MMP12 activity at the surface of activated macrophages. LaRee1 may become a powerful tool for monitoring lung disease.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/59e1b482-dd6e-49ed-adab-af5635812b4a
- author
- Cobos-Correa, Amanda ; Trojanek, Johanna B. ; Diemer, Stefanie LU ; Mall, Marcus A. and Schultz, Carsten
- publishing date
- 2009-09
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Nature Chemical Biology
- volume
- 5
- issue
- 9
- pages
- 628 - 630
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:69249231952
- pmid:19648933
- ISSN
- 1552-4450
- DOI
- 10.1038/nchembio.196
- language
- English
- LU publication?
- no
- id
- 59e1b482-dd6e-49ed-adab-af5635812b4a
- date added to LUP
- 2024-04-29 16:30:59
- date last changed
- 2024-04-29 16:32:04
@article{59e1b482-dd6e-49ed-adab-af5635812b4a, abstract = {{<p>MMP12 is a metalloproteinase implicated in inflammation. To monitor its activity, we synthesized a membrane-targeted reporter (LaRee1) based on Foerster resonance energy transfer (FRET). Unlike existing sensors, LaRee1 detects MMP12 activity by loss of FRET plus internalization of the lipidated fragment. In bronchoalveolar lavages from a mouse model of pulmonary inflammation, LaRee1 detected MMP12 activity at the surface of activated macrophages. LaRee1 may become a powerful tool for monitoring lung disease.</p>}}, author = {{Cobos-Correa, Amanda and Trojanek, Johanna B. and Diemer, Stefanie and Mall, Marcus A. and Schultz, Carsten}}, issn = {{1552-4450}}, language = {{eng}}, number = {{9}}, pages = {{628--630}}, publisher = {{Nature Publishing Group}}, series = {{Nature Chemical Biology}}, title = {{Membrane-bound FRET probe visualizes MMP12 activity in pulmonary inflammation}}, url = {{http://dx.doi.org/10.1038/nchembio.196}}, doi = {{10.1038/nchembio.196}}, volume = {{5}}, year = {{2009}}, }