Detection of vitamin K-dependent proteins in venoms with a monoclonal antibody specific for gamma-carboxyglutamic acid.
(2002) In Toxicon 40(4). p.447-453- Abstract
- gamma-Carboxyglutamic acid (Gla) is an unusual amino acid that is synthesized post-translationally from glutamate in a vitamin K-dependent reaction. The dicarboxylic side chain of Gla chelates Ca(2+), a property important for the biological activity of vitamin K-dependent proteins. To date, Gla-containing polypeptides have been identified in venom from two groups of organisms: elapid snakes, and snails of the genus Conus. In certain elapid snakes, a gamma-carboxylated coagulation factor Xa-like protein is a component of the venom whereas cone snails utilize Gla in a range of peptide neurotoxins. Using a monoclonal antibody that specifically recognizes Gla residues, venom samples from various organisms were screened by western blotting and... (More)
- gamma-Carboxyglutamic acid (Gla) is an unusual amino acid that is synthesized post-translationally from glutamate in a vitamin K-dependent reaction. The dicarboxylic side chain of Gla chelates Ca(2+), a property important for the biological activity of vitamin K-dependent proteins. To date, Gla-containing polypeptides have been identified in venom from two groups of organisms: elapid snakes, and snails of the genus Conus. In certain elapid snakes, a gamma-carboxylated coagulation factor Xa-like protein is a component of the venom whereas cone snails utilize Gla in a range of peptide neurotoxins. Using a monoclonal antibody that specifically recognizes Gla residues, venom samples from various organisms were screened by western blotting and immunofluorescence assays. Amino acid analyses were also performed on most samples. A survey of 21 snake species from 12 genera detected gamma-carboxylated polypeptides only in venom of snakes from the elapid subfamily Acanthophiinae. Gla-containing polypeptides were also observed in cone snail venom but not in venom or toxic salivary secretions from several other organisms. The Gla-specific antibody used here provides a simple immunochemical means to detect gamma-carboxylated polypeptides in venom and may allow new species to be identified that utilize Gla in the biosynthesis of toxic polypeptides. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/107033
- author
- Brown, Mark A. LU ; Hambe, B ; Furie, B ; Furie, B C ; Stenflo, Johan LU and Stenberg, Leisa LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Biological Assay : methods, Anura, Antibodies Monoclonal : diagnostic use, Blood Proteins : metabolism, Elapid Venoms : chemistry, Blotting Western, Factor Xa : analysis : metabolism, Fluorescent Antibody Technique, Mollusk Venoms : chemistry, Peptides : analysis : chemistry, Salivary Glands : chemistry, Support Non-U.S. Gov't, Snakes, Snails, Vitamin K : pharmacology, Animal, Amphibian Venoms : chemistry, 1-Carboxyglutamic Acid : analysis : immunology : pharmacology
- in
- Toxicon
- volume
- 40
- issue
- 4
- pages
- 447 - 453
- publisher
- Elsevier
- external identifiers
-
- pmid:11738238
- wos:000173462600008
- scopus:0036135796
- ISSN
- 0041-0101
- DOI
- 10.1016/S0041-0101(01)00233-1
- language
- English
- LU publication?
- yes
- id
- 5a27c91c-f650-4833-8480-e76c732f7754 (old id 107033)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11738238&dopt=Abstract
- date added to LUP
- 2016-04-01 12:16:19
- date last changed
- 2022-01-27 01:17:32
@article{5a27c91c-f650-4833-8480-e76c732f7754,
abstract = {{gamma-Carboxyglutamic acid (Gla) is an unusual amino acid that is synthesized post-translationally from glutamate in a vitamin K-dependent reaction. The dicarboxylic side chain of Gla chelates Ca(2+), a property important for the biological activity of vitamin K-dependent proteins. To date, Gla-containing polypeptides have been identified in venom from two groups of organisms: elapid snakes, and snails of the genus Conus. In certain elapid snakes, a gamma-carboxylated coagulation factor Xa-like protein is a component of the venom whereas cone snails utilize Gla in a range of peptide neurotoxins. Using a monoclonal antibody that specifically recognizes Gla residues, venom samples from various organisms were screened by western blotting and immunofluorescence assays. Amino acid analyses were also performed on most samples. A survey of 21 snake species from 12 genera detected gamma-carboxylated polypeptides only in venom of snakes from the elapid subfamily Acanthophiinae. Gla-containing polypeptides were also observed in cone snail venom but not in venom or toxic salivary secretions from several other organisms. The Gla-specific antibody used here provides a simple immunochemical means to detect gamma-carboxylated polypeptides in venom and may allow new species to be identified that utilize Gla in the biosynthesis of toxic polypeptides.}},
author = {{Brown, Mark A. and Hambe, B and Furie, B and Furie, B C and Stenflo, Johan and Stenberg, Leisa}},
issn = {{0041-0101}},
keywords = {{Biological Assay : methods; Anura; Antibodies Monoclonal : diagnostic use; Blood Proteins : metabolism; Elapid Venoms : chemistry; Blotting Western; Factor Xa : analysis : metabolism; Fluorescent Antibody Technique; Mollusk Venoms : chemistry; Peptides : analysis : chemistry; Salivary Glands : chemistry; Support Non-U.S. Gov't; Snakes; Snails; Vitamin K : pharmacology; Animal; Amphibian Venoms : chemistry; 1-Carboxyglutamic Acid : analysis : immunology : pharmacology}},
language = {{eng}},
number = {{4}},
pages = {{447--453}},
publisher = {{Elsevier}},
series = {{Toxicon}},
title = {{Detection of vitamin K-dependent proteins in venoms with a monoclonal antibody specific for gamma-carboxyglutamic acid.}},
url = {{http://dx.doi.org/10.1016/S0041-0101(01)00233-1}},
doi = {{10.1016/S0041-0101(01)00233-1}},
volume = {{40}},
year = {{2002}},
}