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Patients with bicuspid and tricuspid aortic valve exhibit distinct regional microrna signatures in mildly dilated ascending aorta

Albinsson, Sebastian LU ; Della Corte, Alessandro; Alajbegovic, Azra LU ; Krawczyk, Katarzyna K. LU ; Bancone, Ciro; Galderisi, Umberto; Cipollaro, Marilena; De Feo, Marisa and Forte, Amalia (2017) In Heart and Vessels 32(6). p.750-767
Abstract

MicroRNAs are able to modulate gene expression in a range of diseases. We focused on microRNAs as potential contributors to the pathogenesis of ascending aorta (AA) dilatation in patients with stenotic tricuspid (TAV) or bicuspid aortic valve (BAV). Aortic specimens were collected from the ‘concavity’ and the ‘convexity’ of mildly dilated AAs and of normal AAs from heart transplant donors. Aortic RNA was analyzed through PCR arrays, profiling the expression of 84 microRNAs involved in cardiovascular disease. An in silico analysis identified the potential microRNA–mRNA interactions and the enriched KEGG pathways potentially affected by microRNA changes in dilated AAs. Distinct signatures of differentially expressed microRNAs are evident... (More)

MicroRNAs are able to modulate gene expression in a range of diseases. We focused on microRNAs as potential contributors to the pathogenesis of ascending aorta (AA) dilatation in patients with stenotic tricuspid (TAV) or bicuspid aortic valve (BAV). Aortic specimens were collected from the ‘concavity’ and the ‘convexity’ of mildly dilated AAs and of normal AAs from heart transplant donors. Aortic RNA was analyzed through PCR arrays, profiling the expression of 84 microRNAs involved in cardiovascular disease. An in silico analysis identified the potential microRNA–mRNA interactions and the enriched KEGG pathways potentially affected by microRNA changes in dilated AAs. Distinct signatures of differentially expressed microRNAs are evident in TAV and BAV patients vs. donors, as well as differences between aortic concavity and convexity in patients only. MicroRNA changes suggest a switch of SMC phenotype, with particular reference to TAV concavity. MicroRNA changes potentially affecting mechanotransduction pathways exhibit a higher prevalence in BAV convexity and in TAV concavity, with particular reference to TGF-β1, Hippo, and PI3K/Akt/FoxO pathways. Actin cytoskeleton emerges as potentially affected by microRNA changes in BAV convexity only. MicroRNAs could play distinct roles in BAV and TAV aortopathy, with possible implications in diagnosis and therapy.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aortopathy, Ascending aorta, Bicuspid aortic valve, Mechanotransduction, MicroRNAs
in
Heart and Vessels
volume
32
issue
6
pages
750 - 767
publisher
Springer
external identifiers
  • scopus:85009889642
  • wos:000402144200014
ISSN
0910-8327
DOI
10.1007/s00380-016-0942-7
language
English
LU publication?
yes
id
5a28174b-50ee-4490-88f1-83a1251de143
date added to LUP
2017-02-06 09:34:25
date last changed
2018-03-11 04:37:10
@article{5a28174b-50ee-4490-88f1-83a1251de143,
  abstract     = {<p>MicroRNAs are able to modulate gene expression in a range of diseases. We focused on microRNAs as potential contributors to the pathogenesis of ascending aorta (AA) dilatation in patients with stenotic tricuspid (TAV) or bicuspid aortic valve (BAV). Aortic specimens were collected from the ‘concavity’ and the ‘convexity’ of mildly dilated AAs and of normal AAs from heart transplant donors. Aortic RNA was analyzed through PCR arrays, profiling the expression of 84 microRNAs involved in cardiovascular disease. An in silico analysis identified the potential microRNA–mRNA interactions and the enriched KEGG pathways potentially affected by microRNA changes in dilated AAs. Distinct signatures of differentially expressed microRNAs are evident in TAV and BAV patients vs. donors, as well as differences between aortic concavity and convexity in patients only. MicroRNA changes suggest a switch of SMC phenotype, with particular reference to TAV concavity. MicroRNA changes potentially affecting mechanotransduction pathways exhibit a higher prevalence in BAV convexity and in TAV concavity, with particular reference to TGF-β1, Hippo, and PI3K/Akt/FoxO pathways. Actin cytoskeleton emerges as potentially affected by microRNA changes in BAV convexity only. MicroRNAs could play distinct roles in BAV and TAV aortopathy, with possible implications in diagnosis and therapy.</p>},
  author       = {Albinsson, Sebastian and Della Corte, Alessandro and Alajbegovic, Azra and Krawczyk, Katarzyna K. and Bancone, Ciro and Galderisi, Umberto and Cipollaro, Marilena and De Feo, Marisa and Forte, Amalia},
  issn         = {0910-8327},
  keyword      = {Aortopathy,Ascending aorta,Bicuspid aortic valve,Mechanotransduction,MicroRNAs},
  language     = {eng},
  month        = {01},
  number       = {6},
  pages        = {750--767},
  publisher    = {Springer},
  series       = {Heart and Vessels},
  title        = {Patients with bicuspid and tricuspid aortic valve exhibit distinct regional microrna signatures in mildly dilated ascending aorta},
  url          = {http://dx.doi.org/10.1007/s00380-016-0942-7},
  volume       = {32},
  year         = {2017},
}