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Affinity processing of cell-containing feeds using monolithic macroporous hydrogels, cryogels.

Galaev, Igor LU and Mattiasson, Bo LU (2008) In Methods in Molecular Biology 421. p.247-255
Abstract
Monolithic macroporous hydrogels, "cryogels," are produced by polymerization in a partially frozen state when the ice crystals perform as a porogen. Cryogels have a unique combination of properties: (i) large (10-100 microm) pores; (ii) minimal non-specific interactions due to the hydrophilic nature of the polymers; (iii) porosities exceeding 80-90%; (iv) good mechanical stability. These properties of cryogels allow for their application for direct capture of extracellularly expressed histidine-tagged protein from the fermentation broth and separation of different cell types.
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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Affinity: methods, Chromatography
in
Methods in Molecular Biology
volume
421
pages
247 - 255
publisher
Springer
external identifiers
  • pmid:18826059
ISSN
1940-6029
DOI
10.1007/978-1-59745-582-4_16
language
English
LU publication?
yes
id
5a5a9314-9281-4ab4-886f-bb2f9a177162 (old id 1262689)
date added to LUP
2016-04-01 12:31:42
date last changed
2018-11-21 20:08:21
@article{5a5a9314-9281-4ab4-886f-bb2f9a177162,
  abstract     = {{Monolithic macroporous hydrogels, "cryogels," are produced by polymerization in a partially frozen state when the ice crystals perform as a porogen. Cryogels have a unique combination of properties: (i) large (10-100 microm) pores; (ii) minimal non-specific interactions due to the hydrophilic nature of the polymers; (iii) porosities exceeding 80-90%; (iv) good mechanical stability. These properties of cryogels allow for their application for direct capture of extracellularly expressed histidine-tagged protein from the fermentation broth and separation of different cell types.}},
  author       = {{Galaev, Igor and Mattiasson, Bo}},
  issn         = {{1940-6029}},
  keywords     = {{Affinity: methods; Chromatography}},
  language     = {{eng}},
  pages        = {{247--255}},
  publisher    = {{Springer}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Affinity processing of cell-containing feeds using monolithic macroporous hydrogels, cryogels.}},
  url          = {{http://dx.doi.org/10.1007/978-1-59745-582-4_16}},
  doi          = {{10.1007/978-1-59745-582-4_16}},
  volume       = {{421}},
  year         = {{2008}},
}