Affinity processing of cell-containing feeds using monolithic macroporous hydrogels, cryogels.
(2008) In Methods in Molecular Biology 421. p.247-255- Abstract
- Monolithic macroporous hydrogels, "cryogels," are produced by polymerization in a partially frozen state when the ice crystals perform as a porogen. Cryogels have a unique combination of properties: (i) large (10-100 microm) pores; (ii) minimal non-specific interactions due to the hydrophilic nature of the polymers; (iii) porosities exceeding 80-90%; (iv) good mechanical stability. These properties of cryogels allow for their application for direct capture of extracellularly expressed histidine-tagged protein from the fermentation broth and separation of different cell types.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1262689
- author
- Galaev, Igor LU and Mattiasson, Bo LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Affinity: methods, Chromatography
- in
- Methods in Molecular Biology
- volume
- 421
- pages
- 247 - 255
- publisher
- Springer
- external identifiers
-
- pmid:18826059
- ISSN
- 1940-6029
- DOI
- 10.1007/978-1-59745-582-4_16
- language
- English
- LU publication?
- yes
- id
- 5a5a9314-9281-4ab4-886f-bb2f9a177162 (old id 1262689)
- date added to LUP
- 2016-04-01 12:31:42
- date last changed
- 2018-11-21 20:08:21
@article{5a5a9314-9281-4ab4-886f-bb2f9a177162, abstract = {{Monolithic macroporous hydrogels, "cryogels," are produced by polymerization in a partially frozen state when the ice crystals perform as a porogen. Cryogels have a unique combination of properties: (i) large (10-100 microm) pores; (ii) minimal non-specific interactions due to the hydrophilic nature of the polymers; (iii) porosities exceeding 80-90%; (iv) good mechanical stability. These properties of cryogels allow for their application for direct capture of extracellularly expressed histidine-tagged protein from the fermentation broth and separation of different cell types.}}, author = {{Galaev, Igor and Mattiasson, Bo}}, issn = {{1940-6029}}, keywords = {{Affinity: methods; Chromatography}}, language = {{eng}}, pages = {{247--255}}, publisher = {{Springer}}, series = {{Methods in Molecular Biology}}, title = {{Affinity processing of cell-containing feeds using monolithic macroporous hydrogels, cryogels.}}, url = {{http://dx.doi.org/10.1007/978-1-59745-582-4_16}}, doi = {{10.1007/978-1-59745-582-4_16}}, volume = {{421}}, year = {{2008}}, }