nfCR1, the first non-LTR retrotransposon characterized in the Australian lungfish genome, Neoceratodus forsteri, shows similarities to CR1-like elements
(2005) In Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 304B(1). p.40-49- Abstract
The genomes of lungfish, together with those of some urodele amphibians, are the largest of all vertebrate genomes. It has been assumed that the bulk of the DNA making up these large genomes has been derived from repeat elements, like the noncoding DNA of those genomes that have been sequenced (e.g., human). In an attempt to characterize repeat sequences in the lungfish genome, we have isolated, by restriction enzyme digestion of genomic DNA, sequences of a repeat element in Neoceratodus forsteri, the most primitive of the living lungfishes. The fragments sequenced from the EcoRI and BglII digests were used to perform genome walking PCR in order to obtain the full sequence of the repeat element. This element shares homology with the... (More)
The genomes of lungfish, together with those of some urodele amphibians, are the largest of all vertebrate genomes. It has been assumed that the bulk of the DNA making up these large genomes has been derived from repeat elements, like the noncoding DNA of those genomes that have been sequenced (e.g., human). In an attempt to characterize repeat sequences in the lungfish genome, we have isolated, by restriction enzyme digestion of genomic DNA, sequences of a repeat element in Neoceratodus forsteri, the most primitive of the living lungfishes. The fragments sequenced from the EcoRI and BglII digests were used to perform genome walking PCR in order to obtain the full sequence of the repeat element. This element shares homology with the non-LTR (LINE) element, Chicken Repeat 1 (CR1), described for several vertebrates and some invertebrates; we have called it N. forsteri CR1 (NfCR1). NfCR1 shares all the domains of other CR1 elements but it also has several unique features that suggest it may no longer be active in the lungfish genome. It occurs in both full-length and 5′-truncated versions and in its present "inactive" form represents ∼0.05% of the lungfish genome.
(Less)
- author
- Sirijovski, Nickolche LU ; Woolnough, Catherine ; Rock, Jennifer and Joss, Jean M.P.
- publishing date
- 2005-01-15
- type
- Contribution to journal
- publication status
- published
- in
- Journal of Experimental Zoology Part B: Molecular and Developmental Evolution
- volume
- 304B
- issue
- 1
- pages
- 40 - 49
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:15593278
- scopus:14844284661
- ISSN
- 1552-5007
- DOI
- 10.1002/jez.b.21022
- language
- English
- LU publication?
- no
- id
- 5a86f6f4-e015-4a21-a724-d17120d595b1
- date added to LUP
- 2019-06-19 12:00:03
- date last changed
- 2024-01-01 11:30:01
@article{5a86f6f4-e015-4a21-a724-d17120d595b1, abstract = {{<p>The genomes of lungfish, together with those of some urodele amphibians, are the largest of all vertebrate genomes. It has been assumed that the bulk of the DNA making up these large genomes has been derived from repeat elements, like the noncoding DNA of those genomes that have been sequenced (e.g., human). In an attempt to characterize repeat sequences in the lungfish genome, we have isolated, by restriction enzyme digestion of genomic DNA, sequences of a repeat element in Neoceratodus forsteri, the most primitive of the living lungfishes. The fragments sequenced from the EcoRI and BglII digests were used to perform genome walking PCR in order to obtain the full sequence of the repeat element. This element shares homology with the non-LTR (LINE) element, Chicken Repeat 1 (CR1), described for several vertebrates and some invertebrates; we have called it N. forsteri CR1 (NfCR1). NfCR1 shares all the domains of other CR1 elements but it also has several unique features that suggest it may no longer be active in the lungfish genome. It occurs in both full-length and 5′-truncated versions and in its present "inactive" form represents ∼0.05% of the lungfish genome.</p>}}, author = {{Sirijovski, Nickolche and Woolnough, Catherine and Rock, Jennifer and Joss, Jean M.P.}}, issn = {{1552-5007}}, language = {{eng}}, month = {{01}}, number = {{1}}, pages = {{40--49}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Journal of Experimental Zoology Part B: Molecular and Developmental Evolution}}, title = {{nfCR1, the first non-LTR retrotransposon characterized in the Australian lungfish genome, Neoceratodus forsteri, shows similarities to CR1-like elements}}, url = {{http://dx.doi.org/10.1002/jez.b.21022}}, doi = {{10.1002/jez.b.21022}}, volume = {{304B}}, year = {{2005}}, }