Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Regulators of floral fragrance production and their target genes in petunia are not exclusively active in the epidermal cells of petals

Van Moerkercke, Alex LU ; Galván-Ampudia, Carlos S ; Verdonk, Julian C ; Haring, Michel A and Schuurink, Robert C (2012) In Journal of Experimental Botany 63(8). p.71-3157
Abstract

In which cells of the flower volatile biosynthesis takes place is unclear. In rose and snapdragon, some enzymes of the volatile phenylpropanoid/benzenoid pathway have been shown to be present in the epidermal cells of petals. It is therefore generally believed that the production of these compounds occurs in these cells. However, whether the entire pathway is active in these cells and whether it is exclusively active in these cells remains to be proven. Cell-specific transcription factors activating these genes will determine in which cells they are expressed. In petunia, the transcription factor EMISSION OF BENZENOIDS II (EOBII) activates the ODORANT1 (ODO1) promoter and the promoter of the biosynthetic gene isoeugenol synthase (IGS).... (More)

In which cells of the flower volatile biosynthesis takes place is unclear. In rose and snapdragon, some enzymes of the volatile phenylpropanoid/benzenoid pathway have been shown to be present in the epidermal cells of petals. It is therefore generally believed that the production of these compounds occurs in these cells. However, whether the entire pathway is active in these cells and whether it is exclusively active in these cells remains to be proven. Cell-specific transcription factors activating these genes will determine in which cells they are expressed. In petunia, the transcription factor EMISSION OF BENZENOIDS II (EOBII) activates the ODORANT1 (ODO1) promoter and the promoter of the biosynthetic gene isoeugenol synthase (IGS). The regulator ODO1 in turn activates the promoter of the shikimate gene 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Here the identification of a new target gene of ODO1, encoding an ABC transporter localized on the plasma membrane, PhABCG1, which is co-expressed with ODO1, is described. PhABCG1 expression is up-regulated in petals overexpressing ODO1 through activation of the PhABCG1 promoter. Interestingly, the ODO1, PhABCG1, and IGS promoters were active in petunia protoplasts originating from both epidermal and mesophyll cell layers of the petal, suggesting that the volatile phenylpropanoid/benzenoid pathway in petunia is active in these different cell types. Since volatile release occurs from epidermal cells, trafficking of (volatile) compounds between cell layers must be involved, but the exact function of PhABCG1 remains to be resolved.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ATP-Binding Cassette Transporters, Amino Acid Sequence, Cell Membrane, Flowers, Gene Expression Regulation, Plant, Genes, Plant, Intracellular Space, Molecular Sequence Data, Molecular Weight, Odorants, Organ Specificity, Petunia, Plant Epidermis, Plant Proteins, Promoter Regions, Genetic, Protein Transport, Protoplasts, RNA, Messenger, Subcellular Fractions
in
Journal of Experimental Botany
volume
63
issue
8
pages
15 pages
publisher
Oxford University Press
external identifiers
  • scopus:84861388522
  • pmid:22345641
ISSN
0022-0957
DOI
10.1093/jxb/ers034
language
English
LU publication?
no
id
5a96b521-8ca4-4a7d-b6cb-5c903a1ab81a
date added to LUP
2017-11-06 11:32:07
date last changed
2024-01-29 06:14:05
@article{5a96b521-8ca4-4a7d-b6cb-5c903a1ab81a,
  abstract     = {{<p>In which cells of the flower volatile biosynthesis takes place is unclear. In rose and snapdragon, some enzymes of the volatile phenylpropanoid/benzenoid pathway have been shown to be present in the epidermal cells of petals. It is therefore generally believed that the production of these compounds occurs in these cells. However, whether the entire pathway is active in these cells and whether it is exclusively active in these cells remains to be proven. Cell-specific transcription factors activating these genes will determine in which cells they are expressed. In petunia, the transcription factor EMISSION OF BENZENOIDS II (EOBII) activates the ODORANT1 (ODO1) promoter and the promoter of the biosynthetic gene isoeugenol synthase (IGS). The regulator ODO1 in turn activates the promoter of the shikimate gene 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Here the identification of a new target gene of ODO1, encoding an ABC transporter localized on the plasma membrane, PhABCG1, which is co-expressed with ODO1, is described. PhABCG1 expression is up-regulated in petals overexpressing ODO1 through activation of the PhABCG1 promoter. Interestingly, the ODO1, PhABCG1, and IGS promoters were active in petunia protoplasts originating from both epidermal and mesophyll cell layers of the petal, suggesting that the volatile phenylpropanoid/benzenoid pathway in petunia is active in these different cell types. Since volatile release occurs from epidermal cells, trafficking of (volatile) compounds between cell layers must be involved, but the exact function of PhABCG1 remains to be resolved.</p>}},
  author       = {{Van Moerkercke, Alex and Galván-Ampudia, Carlos S and Verdonk, Julian C and Haring, Michel A and Schuurink, Robert C}},
  issn         = {{0022-0957}},
  keywords     = {{ATP-Binding Cassette Transporters; Amino Acid Sequence; Cell Membrane; Flowers; Gene Expression Regulation, Plant; Genes, Plant; Intracellular Space; Molecular Sequence Data; Molecular Weight; Odorants; Organ Specificity; Petunia; Plant Epidermis; Plant Proteins; Promoter Regions, Genetic; Protein Transport; Protoplasts; RNA, Messenger; Subcellular Fractions}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{71--3157}},
  publisher    = {{Oxford University Press}},
  series       = {{Journal of Experimental Botany}},
  title        = {{Regulators of floral fragrance production and their target genes in petunia are not exclusively active in the epidermal cells of petals}},
  url          = {{http://dx.doi.org/10.1093/jxb/ers034}},
  doi          = {{10.1093/jxb/ers034}},
  volume       = {{63}},
  year         = {{2012}},
}