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Afrostyrax lepidophyllus Mildbr. and Monodora myristica (Gaertn.) Dunal Extracts Decrease Doxorubicin Cytotoxicity on H9c2 Cardiomyoblasts

Moukette, Bruno M ; Castelão-Baptista, José P ; Ferreira, Luciana ; Silva, Ana M ; Simões, Rui F LU ; Cabral, Célia ; Pieme, Constant A ; Ngogang, Jeanne Y ; Sardão, Vilma A and Oliveira, Paulo J (2021) In Evidence-based Complementary and Alternative Medicine 2021.
Abstract

Materials and Methods: Bark extracts of these plants (1 and 25 µg/mL) were added 3 hours before coincubating H9c2 cardiomyoblasts with Dox (0.5 and 1 µM) for 24 hours more. We measured cell mass and metabolic viability, mitochondrial transmembrane potential, superoxide anion content, and activity-like of caspase-3 and caspase-9 following treatment with the extracts and/or Dox. Also, selenium and vitamin C contents were measured in the plant extracts.

Results: The results confirmed that Dox treatment decreased cell mass, mitochondrial membrane potential and metabolic viability, increased mitochondrial superoxide anion, and stimulated caspase-3 and caspase-9-like activities. Pretreatment of the cells with the plant extracts... (More)

Materials and Methods: Bark extracts of these plants (1 and 25 µg/mL) were added 3 hours before coincubating H9c2 cardiomyoblasts with Dox (0.5 and 1 µM) for 24 hours more. We measured cell mass and metabolic viability, mitochondrial transmembrane potential, superoxide anion content, and activity-like of caspase-3 and caspase-9 following treatment with the extracts and/or Dox. Also, selenium and vitamin C contents were measured in the plant extracts.

Results: The results confirmed that Dox treatment decreased cell mass, mitochondrial membrane potential and metabolic viability, increased mitochondrial superoxide anion, and stimulated caspase-3 and caspase-9-like activities. Pretreatment of the cells with the plant extracts significantly inhibited Dox cytotoxicity, with more significant results at the higher concentration. Measurements of selenium and vitamin C in the extracts revealed higher concentration of both when compared with other Cameroonian spices.

Conclusion: Both extracts of A. lepidophyllus and M. myristica were effective against Dox-induced cytotoxicity, most likely due to their content in antioxidants.

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author
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publishing date
type
Contribution to journal
publication status
published
subject
in
Evidence-based Complementary and Alternative Medicine
volume
2021
article number
8858165
publisher
Hindawi Limited
external identifiers
  • scopus:85102376848
  • pmid:33688366
ISSN
1741-427X
DOI
10.1155/2021/8858165
language
English
LU publication?
no
id
5abab053-4685-4909-a0a8-d9c69ac22161
date added to LUP
2021-09-21 19:21:18
date last changed
2024-06-29 17:43:04
@article{5abab053-4685-4909-a0a8-d9c69ac22161,
  abstract     = {{<p>Materials and Methods: Bark extracts of these plants (1 and 25 µg/mL) were added 3 hours before coincubating H9c2 cardiomyoblasts with Dox (0.5 and 1 µM) for 24 hours more. We measured cell mass and metabolic viability, mitochondrial transmembrane potential, superoxide anion content, and activity-like of caspase-3 and caspase-9 following treatment with the extracts and/or Dox. Also, selenium and vitamin C contents were measured in the plant extracts.</p><p>Results: The results confirmed that Dox treatment decreased cell mass, mitochondrial membrane potential and metabolic viability, increased mitochondrial superoxide anion, and stimulated caspase-3 and caspase-9-like activities. Pretreatment of the cells with the plant extracts significantly inhibited Dox cytotoxicity, with more significant results at the higher concentration. Measurements of selenium and vitamin C in the extracts revealed higher concentration of both when compared with other Cameroonian spices.</p><p>Conclusion: Both extracts of A. lepidophyllus and M. myristica were effective against Dox-induced cytotoxicity, most likely due to their content in antioxidants.</p>}},
  author       = {{Moukette, Bruno M and Castelão-Baptista, José P and Ferreira, Luciana and Silva, Ana M and Simões, Rui F and Cabral, Célia and Pieme, Constant A and Ngogang, Jeanne Y and Sardão, Vilma A and Oliveira, Paulo J}},
  issn         = {{1741-427X}},
  language     = {{eng}},
  publisher    = {{Hindawi Limited}},
  series       = {{Evidence-based Complementary and Alternative Medicine}},
  title        = {{Afrostyrax lepidophyllus Mildbr. and Monodora myristica (Gaertn.) Dunal Extracts Decrease Doxorubicin Cytotoxicity on H9c2 Cardiomyoblasts}},
  url          = {{http://dx.doi.org/10.1155/2021/8858165}},
  doi          = {{10.1155/2021/8858165}},
  volume       = {{2021}},
  year         = {{2021}},
}