Afrostyrax lepidophyllus Mildbr. and Monodora myristica (Gaertn.) Dunal Extracts Decrease Doxorubicin Cytotoxicity on H9c2 Cardiomyoblasts
(2021) In Evidence-based Complementary and Alternative Medicine 2021.- Abstract
Materials and Methods: Bark extracts of these plants (1 and 25 µg/mL) were added 3 hours before coincubating H9c2 cardiomyoblasts with Dox (0.5 and 1 µM) for 24 hours more. We measured cell mass and metabolic viability, mitochondrial transmembrane potential, superoxide anion content, and activity-like of caspase-3 and caspase-9 following treatment with the extracts and/or Dox. Also, selenium and vitamin C contents were measured in the plant extracts.
Results: The results confirmed that Dox treatment decreased cell mass, mitochondrial membrane potential and metabolic viability, increased mitochondrial superoxide anion, and stimulated caspase-3 and caspase-9-like activities. Pretreatment of the cells with the plant extracts... (More)
Materials and Methods: Bark extracts of these plants (1 and 25 µg/mL) were added 3 hours before coincubating H9c2 cardiomyoblasts with Dox (0.5 and 1 µM) for 24 hours more. We measured cell mass and metabolic viability, mitochondrial transmembrane potential, superoxide anion content, and activity-like of caspase-3 and caspase-9 following treatment with the extracts and/or Dox. Also, selenium and vitamin C contents were measured in the plant extracts.
Results: The results confirmed that Dox treatment decreased cell mass, mitochondrial membrane potential and metabolic viability, increased mitochondrial superoxide anion, and stimulated caspase-3 and caspase-9-like activities. Pretreatment of the cells with the plant extracts significantly inhibited Dox cytotoxicity, with more significant results at the higher concentration. Measurements of selenium and vitamin C in the extracts revealed higher concentration of both when compared with other Cameroonian spices.
Conclusion: Both extracts of A. lepidophyllus and M. myristica were effective against Dox-induced cytotoxicity, most likely due to their content in antioxidants.
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- author
- Moukette, Bruno M ; Castelão-Baptista, José P ; Ferreira, Luciana ; Silva, Ana M ; Simões, Rui F LU ; Cabral, Célia ; Pieme, Constant A ; Ngogang, Jeanne Y ; Sardão, Vilma A and Oliveira, Paulo J
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Evidence-based Complementary and Alternative Medicine
- volume
- 2021
- article number
- 8858165
- publisher
- Hindawi Limited
- external identifiers
-
- scopus:85102376848
- pmid:33688366
- ISSN
- 1741-427X
- DOI
- 10.1155/2021/8858165
- language
- English
- LU publication?
- no
- id
- 5abab053-4685-4909-a0a8-d9c69ac22161
- date added to LUP
- 2021-09-21 19:21:18
- date last changed
- 2024-07-27 20:58:51
@article{5abab053-4685-4909-a0a8-d9c69ac22161,
abstract = {{<p>Materials and Methods: Bark extracts of these plants (1 and 25 µg/mL) were added 3 hours before coincubating H9c2 cardiomyoblasts with Dox (0.5 and 1 µM) for 24 hours more. We measured cell mass and metabolic viability, mitochondrial transmembrane potential, superoxide anion content, and activity-like of caspase-3 and caspase-9 following treatment with the extracts and/or Dox. Also, selenium and vitamin C contents were measured in the plant extracts.</p><p>Results: The results confirmed that Dox treatment decreased cell mass, mitochondrial membrane potential and metabolic viability, increased mitochondrial superoxide anion, and stimulated caspase-3 and caspase-9-like activities. Pretreatment of the cells with the plant extracts significantly inhibited Dox cytotoxicity, with more significant results at the higher concentration. Measurements of selenium and vitamin C in the extracts revealed higher concentration of both when compared with other Cameroonian spices.</p><p>Conclusion: Both extracts of A. lepidophyllus and M. myristica were effective against Dox-induced cytotoxicity, most likely due to their content in antioxidants.</p>}},
author = {{Moukette, Bruno M and Castelão-Baptista, José P and Ferreira, Luciana and Silva, Ana M and Simões, Rui F and Cabral, Célia and Pieme, Constant A and Ngogang, Jeanne Y and Sardão, Vilma A and Oliveira, Paulo J}},
issn = {{1741-427X}},
language = {{eng}},
publisher = {{Hindawi Limited}},
series = {{Evidence-based Complementary and Alternative Medicine}},
title = {{Afrostyrax lepidophyllus Mildbr. and Monodora myristica (Gaertn.) Dunal Extracts Decrease Doxorubicin Cytotoxicity on H9c2 Cardiomyoblasts}},
url = {{http://dx.doi.org/10.1155/2021/8858165}},
doi = {{10.1155/2021/8858165}},
volume = {{2021}},
year = {{2021}},
}