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Analysis of bacterial surface interactions with mass spectrometry-based proteomics

Karlsson, Christofer LU ; Teleman, Johan LU and Malmström, Johan LU (2017) In Methods in Molecular Biology 1535. p.17-24
Abstract

Host–pathogen protein–protein interaction networks are highly complex and dynamic. In this experimental protocol we describe a method to isolate host proteins attached to the bacterial surface followed by quantitative mass spectrometry based proteomics analysis. This technique provides an overview of the host–pathogen interaction network, which can be used to guide directed perturbations of the system, and to select target of specific interest for further studies.

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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Bacteria, Bioinformatics, Mass spectrometry, Peptide solid phase extraction, Proteomics, Surface absorption, Trypsin digestion
in
Methods in Molecular Biology
volume
1535
pages
8 pages
publisher
Humana Press
external identifiers
  • scopus:85005958989
ISSN
10643745
DOI
10.1007/978-1-4939-6673-8_2
language
English
LU publication?
yes
id
5b0780c8-58e1-472b-91b8-cca17205cc60
date added to LUP
2017-03-16 13:02:09
date last changed
2018-01-21 04:20:21
@inbook{5b0780c8-58e1-472b-91b8-cca17205cc60,
  abstract     = {<p>Host–pathogen protein–protein interaction networks are highly complex and dynamic. In this experimental protocol we describe a method to isolate host proteins attached to the bacterial surface followed by quantitative mass spectrometry based proteomics analysis. This technique provides an overview of the host–pathogen interaction network, which can be used to guide directed perturbations of the system, and to select target of specific interest for further studies.</p>},
  author       = {Karlsson, Christofer and Teleman, Johan and Malmström, Johan},
  issn         = {10643745},
  keyword      = {Bacteria,Bioinformatics,Mass spectrometry,Peptide solid phase extraction,Proteomics,Surface absorption,Trypsin digestion},
  language     = {eng},
  pages        = {17--24},
  publisher    = {Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Analysis of bacterial surface interactions with mass spectrometry-based proteomics},
  url          = {http://dx.doi.org/10.1007/978-1-4939-6673-8_2},
  volume       = {1535},
  year         = {2017},
}