Analysis of bacterial surface interactions with mass spectrometry-based proteomics
Karlsson, Christofer LU ; Teleman, Johan LU and Malmström, Johan LU
(2017)
In Methods in Molecular Biology
1535.
p.17-24
- Abstract
Host–pathogen protein–protein interaction networks are highly complex and dynamic. In this experimental protocol we describe a method to isolate host proteins attached to the bacterial surface followed by quantitative mass spectrometry based proteomics analysis. This technique provides an overview of the host–pathogen interaction network, which can be used to guide directed perturbations of the system, and to select target of specific interest for further studies.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5b0780c8-58e1-472b-91b8-cca17205cc60
- author
- Karlsson, Christofer
LU
; Teleman, Johan
LU
and Malmström, Johan
LU
- organization
- publishing date
- 2017
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Bacteria, Bioinformatics, Mass spectrometry, Peptide solid phase extraction, Proteomics, Surface absorption, Trypsin digestion
- host publication
- Methods in Molecular Biology
- series title
- Methods in Molecular Biology
- volume
- 1535
- pages
- 8 pages
- publisher
- Humana Press
- external identifiers
-
- scopus:85005958989
- pmid:27914070
- ISSN
- 10643745
- DOI
- 10.1007/978-1-4939-6673-8_2
- language
- English
- LU publication?
- yes
- id
- 5b0780c8-58e1-472b-91b8-cca17205cc60
- date added to LUP
- 2017-03-16 13:02:09
- date last changed
- 2024-03-17 10:45:10
@inbook{5b0780c8-58e1-472b-91b8-cca17205cc60,
abstract = {{<p>Host–pathogen protein–protein interaction networks are highly complex and dynamic. In this experimental protocol we describe a method to isolate host proteins attached to the bacterial surface followed by quantitative mass spectrometry based proteomics analysis. This technique provides an overview of the host–pathogen interaction network, which can be used to guide directed perturbations of the system, and to select target of specific interest for further studies.</p>}},
author = {{Karlsson, Christofer and Teleman, Johan and Malmström, Johan}},
booktitle = {{Methods in Molecular Biology}},
issn = {{10643745}},
keywords = {{Bacteria; Bioinformatics; Mass spectrometry; Peptide solid phase extraction; Proteomics; Surface absorption; Trypsin digestion}},
language = {{eng}},
pages = {{17--24}},
publisher = {{Humana Press}},
series = {{Methods in Molecular Biology}},
title = {{Analysis of bacterial surface interactions with mass spectrometry-based proteomics}},
url = {{http://dx.doi.org/10.1007/978-1-4939-6673-8_2}},
doi = {{10.1007/978-1-4939-6673-8_2}},
volume = {{1535}},
year = {{2017}},
}