Treatment with cyclosporin A during sensitization with trimellitic anhydride attenuates the airway responses to allergen challenge three weeks later
(1994) In European Journal of Pharmacology 252(3). p.313-319- Abstract
- The present studies examined the effects of oral treatment with cyclosporin A, betamethasone or azelastine administered over the time of sensitization with trimellitic anhydride on allergen-induced airway responses, compared to those of control animals given corn oil alone. Drugs were given for 8 days. The animals were sensitized with trimellitic anhydride (0.1 ml of 0.3% w/v) in corn oil given intradermally on days 4 and 5 of drug treatment. Three to four weeks after sensitization with free trimellitic anhydride, the animals were anesthetized, tracheostomized and challenged with trimellitic anhydride conjugated to guinea pig serum albumin (trimellitic anhydride-guinea pig serum albumin; 0.5%; 50 microliters) instilled via the airway... (More)
- The present studies examined the effects of oral treatment with cyclosporin A, betamethasone or azelastine administered over the time of sensitization with trimellitic anhydride on allergen-induced airway responses, compared to those of control animals given corn oil alone. Drugs were given for 8 days. The animals were sensitized with trimellitic anhydride (0.1 ml of 0.3% w/v) in corn oil given intradermally on days 4 and 5 of drug treatment. Three to four weeks after sensitization with free trimellitic anhydride, the animals were anesthetized, tracheostomized and challenged with trimellitic anhydride conjugated to guinea pig serum albumin (trimellitic anhydride-guinea pig serum albumin; 0.5%; 50 microliters) instilled via the airway route. In the same animal, we measured both lung resistance (RL) to monitor airflow obstruction, and extravasation of Evans Blue dye (20 mg/kg) to quantify airway plasma exudation. In control animals, instillation of trimellitic anhydride-guinea pig serum albumin into the tracheal lumen caused a slowly progressing increase in RL over the observation period (6 min), in addition to extravasation of Evans Blue dye at all airway levels. In animals treated with 50 mg/kg of cyclosporin A, both the allergen-induced increase in RL and extravasation of Evans Blue dye in intrapulmonary airways were significantly attenuated. However, neither betamethasone nor azelastine significantly affected these responses. We conclude that cyclosporin A may influence the immune system in the guinea pig during the induction of allergy, thus leading to attenuation of allergen-induced airway obstruction at later time points. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1108313
- author
- Arakawa, H ; Andius, P ; Kawikova, I ; Skoogh, B E ; Löfdahl, Claes-Göran LU and Lotvall, J
- publishing date
- 1994
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Asthma, Anhydride, Allergy, Cyclosporin A, Glucocorticoid, Sensitization
- in
- European Journal of Pharmacology
- volume
- 252
- issue
- 3
- pages
- 313 - 319
- publisher
- Elsevier
- external identifiers
-
- pmid:7909293
- scopus:0028137899
- ISSN
- 1879-0712
- DOI
- 10.1016/0014-2999(94)90178-3
- language
- English
- LU publication?
- no
- id
- 5b39e3b2-21d0-4369-9f37-6f50a326ac71 (old id 1108313)
- date added to LUP
- 2016-04-01 12:02:11
- date last changed
- 2021-01-03 10:56:03
@article{5b39e3b2-21d0-4369-9f37-6f50a326ac71,
abstract = {{The present studies examined the effects of oral treatment with cyclosporin A, betamethasone or azelastine administered over the time of sensitization with trimellitic anhydride on allergen-induced airway responses, compared to those of control animals given corn oil alone. Drugs were given for 8 days. The animals were sensitized with trimellitic anhydride (0.1 ml of 0.3% w/v) in corn oil given intradermally on days 4 and 5 of drug treatment. Three to four weeks after sensitization with free trimellitic anhydride, the animals were anesthetized, tracheostomized and challenged with trimellitic anhydride conjugated to guinea pig serum albumin (trimellitic anhydride-guinea pig serum albumin; 0.5%; 50 microliters) instilled via the airway route. In the same animal, we measured both lung resistance (RL) to monitor airflow obstruction, and extravasation of Evans Blue dye (20 mg/kg) to quantify airway plasma exudation. In control animals, instillation of trimellitic anhydride-guinea pig serum albumin into the tracheal lumen caused a slowly progressing increase in RL over the observation period (6 min), in addition to extravasation of Evans Blue dye at all airway levels. In animals treated with 50 mg/kg of cyclosporin A, both the allergen-induced increase in RL and extravasation of Evans Blue dye in intrapulmonary airways were significantly attenuated. However, neither betamethasone nor azelastine significantly affected these responses. We conclude that cyclosporin A may influence the immune system in the guinea pig during the induction of allergy, thus leading to attenuation of allergen-induced airway obstruction at later time points.}},
author = {{Arakawa, H and Andius, P and Kawikova, I and Skoogh, B E and Löfdahl, Claes-Göran and Lotvall, J}},
issn = {{1879-0712}},
keywords = {{Asthma; Anhydride; Allergy; Cyclosporin A; Glucocorticoid; Sensitization}},
language = {{eng}},
number = {{3}},
pages = {{313--319}},
publisher = {{Elsevier}},
series = {{European Journal of Pharmacology}},
title = {{Treatment with cyclosporin A during sensitization with trimellitic anhydride attenuates the airway responses to allergen challenge three weeks later}},
url = {{http://dx.doi.org/10.1016/0014-2999(94)90178-3}},
doi = {{10.1016/0014-2999(94)90178-3}},
volume = {{252}},
year = {{1994}},
}