A simple protocol to obtain highly pure Wolbachia endosymbiont DNA for genome sequencing
(2011) In Journal of Microbiological Methods 84(1). p.134-136- Abstract
- Most genome sequencing projects using intracellular bacteria face difficulties in obtaining sufficient bacterial DNA free of host contamination. We have developed a simple and rapid protocol to isolate endosymbiont DNA virtually free from fly and mosquito host DNA. We purified DNA from six Wolbachia strains in preparation for genome sequencing using this method, and achieved up to 97% pure Wolbachia sequence, even after using frozen insects. This is a significant improvement for future Wolbachia and other endosymbiont genome projects.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5bedc48a-7df3-4f47-b05e-2decd40c5b0b
- author
- Iturbe-Ormaetxe, Inaki ; Woolfit, Megan ; Rances, Edwige ; Duplouy, Anne LU and O'NEill, Scott L.
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Microbiological Methods
- volume
- 84
- issue
- 1
- pages
- 134 - 136
- publisher
- Elsevier
- external identifiers
-
- scopus:78650192123
- ISSN
- 1872-8359
- DOI
- 10.1016/j.mimet.2010.10.019
- language
- English
- LU publication?
- no
- id
- 5bedc48a-7df3-4f47-b05e-2decd40c5b0b
- date added to LUP
- 2018-11-12 14:39:01
- date last changed
- 2022-01-31 06:56:37
@article{5bedc48a-7df3-4f47-b05e-2decd40c5b0b, abstract = {{Most genome sequencing projects using intracellular bacteria face difficulties in obtaining sufficient bacterial DNA free of host contamination. We have developed a simple and rapid protocol to isolate endosymbiont DNA virtually free from fly and mosquito host DNA. We purified DNA from six Wolbachia strains in preparation for genome sequencing using this method, and achieved up to 97% pure Wolbachia sequence, even after using frozen insects. This is a significant improvement for future Wolbachia and other endosymbiont genome projects.}}, author = {{Iturbe-Ormaetxe, Inaki and Woolfit, Megan and Rances, Edwige and Duplouy, Anne and O'NEill, Scott L.}}, issn = {{1872-8359}}, language = {{eng}}, number = {{1}}, pages = {{134--136}}, publisher = {{Elsevier}}, series = {{Journal of Microbiological Methods}}, title = {{A simple protocol to obtain highly pure Wolbachia endosymbiont DNA for genome sequencing}}, url = {{http://dx.doi.org/10.1016/j.mimet.2010.10.019}}, doi = {{10.1016/j.mimet.2010.10.019}}, volume = {{84}}, year = {{2011}}, }