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A simple protocol to obtain highly pure Wolbachia endosymbiont DNA for genome sequencing

Iturbe-Ormaetxe, Inaki; Woolfit, Megan; Rances, Edwige; Duplouy, Anne LU and O'NEill, Scott L. (2011) In Journal of Microbiological Methods 84(1). p.134-136
Abstract
Most genome sequencing projects using intracellular bacteria face difficulties in obtaining sufficient bacterial DNA free of host contamination. We have developed a simple and rapid protocol to isolate endosymbiont DNA virtually free from fly and mosquito host DNA. We purified DNA from six Wolbachia strains in preparation for genome sequencing using this method, and achieved up to 97% pure Wolbachia sequence, even after using frozen insects. This is a significant improvement for future Wolbachia and other endosymbiont genome projects.
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author
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Microbiological Methods
volume
84
issue
1
pages
134 - 136
publisher
Elsevier
ISSN
1872-8359
DOI
10.1016/j.mimet.2010.10.019
language
English
LU publication?
no
id
5bedc48a-7df3-4f47-b05e-2decd40c5b0b
date added to LUP
2018-11-12 14:39:01
date last changed
2019-08-13 04:04:20
@article{5bedc48a-7df3-4f47-b05e-2decd40c5b0b,
  abstract     = {Most genome sequencing projects using intracellular bacteria face difficulties in obtaining sufficient bacterial DNA free of host contamination. We have developed a simple and rapid protocol to isolate endosymbiont DNA virtually free from fly and mosquito host DNA. We purified DNA from six Wolbachia strains in preparation for genome sequencing using this method, and achieved up to 97% pure Wolbachia sequence, even after using frozen insects. This is a significant improvement for future Wolbachia and other endosymbiont genome projects.},
  author       = {Iturbe-Ormaetxe, Inaki and Woolfit, Megan and Rances, Edwige and Duplouy, Anne and O'NEill, Scott L. },
  issn         = {1872-8359},
  language     = {eng},
  number       = {1},
  pages        = {134--136},
  publisher    = {Elsevier},
  series       = {Journal of Microbiological Methods},
  title        = {A simple protocol to obtain highly pure Wolbachia endosymbiont DNA for genome sequencing},
  url          = {http://dx.doi.org/10.1016/j.mimet.2010.10.019},
  volume       = {84},
  year         = {2011},
}