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Comparative proteome analysis of Mycobacterium tuberculosis grown under aerobic and anaerobic conditions

Starck, Joakim LU ; Källenius, Gunilla; Marklund, Britt-Inger; Andersson, Dan I. and Åkerlund, Thomas (2004) In Microbiology1994-01-01+01:00 150(11). p.3821-3829
Abstract

Data are presented from two-dimensional (2-D) PAGE analysis of Mycobacterium tuberculosis strain Harlingen grown during aerobic and anaerobic culture, according to a modified Wayne dormancy model. M. tuberculosis cultures were grown to the transition point between exponential growth and stationary phase in the presence of oxygen (7 days) and then part of the cultures was shifted to anaerobic conditions for 16 days. Growth declined similarly during aerobic and anaerobic conditions, whereas the ATP consumption rapidly decreased in the anaerobic cultures. 2-D PAGE revealed 50 protein spots that were either unique to, or more abundant during, anaerobic conditions and 16 of these were identified by MALDI-TOF. These proteins were the... (More)

Data are presented from two-dimensional (2-D) PAGE analysis of Mycobacterium tuberculosis strain Harlingen grown during aerobic and anaerobic culture, according to a modified Wayne dormancy model. M. tuberculosis cultures were grown to the transition point between exponential growth and stationary phase in the presence of oxygen (7 days) and then part of the cultures was shifted to anaerobic conditions for 16 days. Growth declined similarly during aerobic and anaerobic conditions, whereas the ATP consumption rapidly decreased in the anaerobic cultures. 2-D PAGE revealed 50 protein spots that were either unique to, or more abundant during, anaerobic conditions and 16 of these were identified by MALDI-TOF. These proteins were the α-crystalline homologue (HspX), elongation factor Tu (Tuf), GroEL2, succinyl-CoA: 3-oxoacid-CoA transferase (ScoB), mycolic acid synthase (CmaA2), thioredoxin (TrxB2), β-ketoacyl-ACP synthase (KasB), L-alanine dehydrogenase (Ald), Rv2005c, Rv2629, Rv0560c, Rv2185c and Rv3866. Some protein spots were found to be proteolytic fragments, e.g. HspX and GroEL2. These data suggest that M. tuberculosis induces expression of about 1% of its genes in response to dormancy.

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author
publishing date
type
Contribution to journal
publication status
published
in
Microbiology1994-01-01+01:00
volume
150
issue
11
pages
9 pages
publisher
MAIK Nauka/Interperiodica
external identifiers
  • scopus:9144263752
ISSN
1350-0872
DOI
10.1099/mic.0.27284-0
language
English
LU publication?
no
id
5bf89247-f2b6-4229-aae0-5ea14dba1ccd
date added to LUP
2017-10-26 15:51:15
date last changed
2017-12-10 05:12:59
@article{5bf89247-f2b6-4229-aae0-5ea14dba1ccd,
  abstract     = {<p>Data are presented from two-dimensional (2-D) PAGE analysis of Mycobacterium tuberculosis strain Harlingen grown during aerobic and anaerobic culture, according to a modified Wayne dormancy model. M. tuberculosis cultures were grown to the transition point between exponential growth and stationary phase in the presence of oxygen (7 days) and then part of the cultures was shifted to anaerobic conditions for 16 days. Growth declined similarly during aerobic and anaerobic conditions, whereas the ATP consumption rapidly decreased in the anaerobic cultures. 2-D PAGE revealed 50 protein spots that were either unique to, or more abundant during, anaerobic conditions and 16 of these were identified by MALDI-TOF. These proteins were the α-crystalline homologue (HspX), elongation factor Tu (Tuf), GroEL2, succinyl-CoA: 3-oxoacid-CoA transferase (ScoB), mycolic acid synthase (CmaA2), thioredoxin (TrxB2), β-ketoacyl-ACP synthase (KasB), L-alanine dehydrogenase (Ald), Rv2005c, Rv2629, Rv0560c, Rv2185c and Rv3866. Some protein spots were found to be proteolytic fragments, e.g. HspX and GroEL2. These data suggest that M. tuberculosis induces expression of about 1% of its genes in response to dormancy.</p>},
  author       = {Starck, Joakim and Källenius, Gunilla and Marklund, Britt-Inger and Andersson, Dan I. and Åkerlund, Thomas},
  issn         = {1350-0872},
  language     = {eng},
  number       = {11},
  pages        = {3821--3829},
  publisher    = {MAIK Nauka/Interperiodica},
  series       = {Microbiology1994-01-01+01:00},
  title        = {Comparative proteome analysis of Mycobacterium tuberculosis grown under aerobic and anaerobic conditions},
  url          = {http://dx.doi.org/10.1099/mic.0.27284-0},
  volume       = {150},
  year         = {2004},
}