In Vivo Profiling of the Vascular Cell Surface Proteome in Murine Models of Bacteremia
(2023) In Methods in molecular biology (Clifton, N.J.) p.285-293- Abstract
Vascular dysfunction is a hallmark of systemic inflammatory responses such as bacterial sepsis. The luminal surface of the blood vessels is coated with a dense layer of glycans and proteoglycans, collectively known as the glycocalyx. Surface associated glycoproteins of endothelial origin, or derived from pericytes, intravascular leukocytes, and plasma, are other important components of the glycocalyx, constituting a vascular cell surface proteome that is dynamic, tissue-specific, and sensitive to changes in vascular homeostasis, blood infection, and inflammation. Here, we describe an experimental protocol to chemically tag and quantify the vascular cell surface proteome in murine models of bacteremia, in a time-resolved and... (More)
Vascular dysfunction is a hallmark of systemic inflammatory responses such as bacterial sepsis. The luminal surface of the blood vessels is coated with a dense layer of glycans and proteoglycans, collectively known as the glycocalyx. Surface associated glycoproteins of endothelial origin, or derived from pericytes, intravascular leukocytes, and plasma, are other important components of the glycocalyx, constituting a vascular cell surface proteome that is dynamic, tissue-specific, and sensitive to changes in vascular homeostasis, blood infection, and inflammation. Here, we describe an experimental protocol to chemically tag and quantify the vascular cell surface proteome in murine models of bacteremia, in a time-resolved and organ-specific manner. This method facilitates the identification of markers of vascular activation and provides a molecular framework to understand the contribution of vascular dysfunction to the organ pathology of systemic inflammation.
(Less)
- author
- Spliid, Charlotte ; Esko, Jeffrey D ; Malmström, Johan LU and Toledo, Alejandro Gomez LU
- organization
- publishing date
- 2023
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Humans, Animals, Mice, Proteome/metabolism, Disease Models, Animal, Glycocalyx/pathology, Bacteremia, Inflammation/metabolism, Endothelium, Vascular
- host publication
- Bacterial pathogenesis : Methods and protocols - Methods and protocols
- series title
- Methods in molecular biology (Clifton, N.J.)
- editor
- Nordenfelt, Pontus and Collin, Mattias
- edition
- 2
- pages
- 285 - 293
- publisher
- Humana Press
- external identifiers
-
- pmid:37258975
- scopus:85160703776
- ISSN
- 1940-6029
- ISBN
- 978-1-0716-3242-0
- 978-1-0716-3243-7
- DOI
- 10.1007/978-1-0716-3243-7_19
- language
- English
- LU publication?
- yes
- id
- 5bfc2079-65c2-4b3f-810f-d8e25843cdd2
- date added to LUP
- 2023-06-05 13:25:38
- date last changed
- 2024-04-05 20:16:16
@inbook{5bfc2079-65c2-4b3f-810f-d8e25843cdd2, abstract = {{<p>Vascular dysfunction is a hallmark of systemic inflammatory responses such as bacterial sepsis. The luminal surface of the blood vessels is coated with a dense layer of glycans and proteoglycans, collectively known as the glycocalyx. Surface associated glycoproteins of endothelial origin, or derived from pericytes, intravascular leukocytes, and plasma, are other important components of the glycocalyx, constituting a vascular cell surface proteome that is dynamic, tissue-specific, and sensitive to changes in vascular homeostasis, blood infection, and inflammation. Here, we describe an experimental protocol to chemically tag and quantify the vascular cell surface proteome in murine models of bacteremia, in a time-resolved and organ-specific manner. This method facilitates the identification of markers of vascular activation and provides a molecular framework to understand the contribution of vascular dysfunction to the organ pathology of systemic inflammation.</p>}}, author = {{Spliid, Charlotte and Esko, Jeffrey D and Malmström, Johan and Toledo, Alejandro Gomez}}, booktitle = {{Bacterial pathogenesis : Methods and protocols}}, editor = {{Nordenfelt, Pontus and Collin, Mattias}}, isbn = {{978-1-0716-3242-0}}, issn = {{1940-6029}}, keywords = {{Humans; Animals; Mice; Proteome/metabolism; Disease Models, Animal; Glycocalyx/pathology; Bacteremia; Inflammation/metabolism; Endothelium, Vascular}}, language = {{eng}}, pages = {{285--293}}, publisher = {{Humana Press}}, series = {{Methods in molecular biology (Clifton, N.J.)}}, title = {{In Vivo Profiling of the Vascular Cell Surface Proteome in Murine Models of Bacteremia}}, url = {{http://dx.doi.org/10.1007/978-1-0716-3243-7_19}}, doi = {{10.1007/978-1-0716-3243-7_19}}, year = {{2023}}, }