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In Vivo Profiling of the Vascular Cell Surface Proteome in Murine Models of Bacteremia

Spliid, Charlotte ; Esko, Jeffrey D ; Malmström, Johan LU orcid and Toledo, Alejandro Gomez LU (2023) In Methods in molecular biology (Clifton, N.J.) p.285-293
Abstract

Vascular dysfunction is a hallmark of systemic inflammatory responses such as bacterial sepsis. The luminal surface of the blood vessels is coated with a dense layer of glycans and proteoglycans, collectively known as the glycocalyx. Surface associated glycoproteins of endothelial origin, or derived from pericytes, intravascular leukocytes, and plasma, are other important components of the glycocalyx, constituting a vascular cell surface proteome that is dynamic, tissue-specific, and sensitive to changes in vascular homeostasis, blood infection, and inflammation. Here, we describe an experimental protocol to chemically tag and quantify the vascular cell surface proteome in murine models of bacteremia, in a time-resolved and... (More)

Vascular dysfunction is a hallmark of systemic inflammatory responses such as bacterial sepsis. The luminal surface of the blood vessels is coated with a dense layer of glycans and proteoglycans, collectively known as the glycocalyx. Surface associated glycoproteins of endothelial origin, or derived from pericytes, intravascular leukocytes, and plasma, are other important components of the glycocalyx, constituting a vascular cell surface proteome that is dynamic, tissue-specific, and sensitive to changes in vascular homeostasis, blood infection, and inflammation. Here, we describe an experimental protocol to chemically tag and quantify the vascular cell surface proteome in murine models of bacteremia, in a time-resolved and organ-specific manner. This method facilitates the identification of markers of vascular activation and provides a molecular framework to understand the contribution of vascular dysfunction to the organ pathology of systemic inflammation.

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Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Humans, Animals, Mice, Proteome/metabolism, Disease Models, Animal, Glycocalyx/pathology, Bacteremia, Inflammation/metabolism, Endothelium, Vascular
host publication
Bacterial pathogenesis : Methods and protocols - Methods and protocols
series title
Methods in molecular biology (Clifton, N.J.)
editor
Nordenfelt, Pontus and Collin, Mattias
edition
2
pages
285 - 293
publisher
Humana Press
external identifiers
  • pmid:37258975
  • scopus:85160703776
ISSN
1940-6029
ISBN
978-1-0716-3242-0
978-1-0716-3243-7
DOI
10.1007/978-1-0716-3243-7_19
language
English
LU publication?
yes
id
5bfc2079-65c2-4b3f-810f-d8e25843cdd2
date added to LUP
2023-06-05 13:25:38
date last changed
2024-04-05 20:16:16
@inbook{5bfc2079-65c2-4b3f-810f-d8e25843cdd2,
  abstract     = {{<p>Vascular dysfunction is a hallmark of systemic inflammatory responses such as bacterial sepsis. The luminal surface of the blood vessels is coated with a dense layer of glycans and proteoglycans, collectively known as the glycocalyx. Surface associated glycoproteins of endothelial origin, or derived from pericytes, intravascular leukocytes, and plasma, are other important components of the glycocalyx, constituting a vascular cell surface proteome that is dynamic, tissue-specific, and sensitive to changes in vascular homeostasis, blood infection, and inflammation. Here, we describe an experimental protocol to chemically tag and quantify the vascular cell surface proteome in murine models of bacteremia, in a time-resolved and organ-specific manner. This method facilitates the identification of markers of vascular activation and provides a molecular framework to understand the contribution of vascular dysfunction to the organ pathology of systemic inflammation.</p>}},
  author       = {{Spliid, Charlotte and Esko, Jeffrey D and Malmström, Johan and Toledo, Alejandro Gomez}},
  booktitle    = {{Bacterial pathogenesis : Methods and protocols}},
  editor       = {{Nordenfelt, Pontus and Collin, Mattias}},
  isbn         = {{978-1-0716-3242-0}},
  issn         = {{1940-6029}},
  keywords     = {{Humans; Animals; Mice; Proteome/metabolism; Disease Models, Animal; Glycocalyx/pathology; Bacteremia; Inflammation/metabolism; Endothelium, Vascular}},
  language     = {{eng}},
  pages        = {{285--293}},
  publisher    = {{Humana Press}},
  series       = {{Methods in molecular biology (Clifton, N.J.)}},
  title        = {{In Vivo Profiling of the Vascular Cell Surface Proteome in Murine Models of Bacteremia}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-3243-7_19}},
  doi          = {{10.1007/978-1-0716-3243-7_19}},
  year         = {{2023}},
}