Susceptibility to insulin-dependent diabetes defined by restriction enzyme polymorphism of HLA-D region genomic DNA
Owerbach, D. ; Hagglof, B. ; Lernmark, A. LU
and Holmgren, G.
(1984)
In Diabetes
33(10).
p.958-965
- Abstract
DNA fragments complementary to cloned sequences encoding HLA-D region class II antigen α- and β-chains were determined by clotting with DNA from HLA-typed members of 22 complete families, 12 of which had a proband with insulin-dependent diabetes mellitus (IDDM). Analysis of genotypes showed that the DNA sequences were linked to HLA-DR and permitted confirmation of recombinations in two families. Digestion with the restriction enzymes BamHI, EcoRI, and Pstl and hybridization with an HLA-D region β-chain cDNA probe confirmed a BamHI 3.7 kilobase (kb) fragment present at low frequency among diabetic individuals and a BamHI 3.2 kb fragment that was also decreased among the diabetic subjects compared with siblings (P < 0.05) as well as... (More)
DNA fragments complementary to cloned sequences encoding HLA-D region class II antigen α- and β-chains were determined by clotting with DNA from HLA-typed members of 22 complete families, 12 of which had a proband with insulin-dependent diabetes mellitus (IDDM). Analysis of genotypes showed that the DNA sequences were linked to HLA-DR and permitted confirmation of recombinations in two families. Digestion with the restriction enzymes BamHI, EcoRI, and Pstl and hybridization with an HLA-D region β-chain cDNA probe confirmed a BamHI 3.7 kilobase (kb) fragment present at low frequency among diabetic individuals and a BamHI 3.2 kb fragment that was also decreased among the diabetic subjects compared with siblings (P < 0.05) as well as nonrelated control siblings (P < 0.02) and their parents (P < 0.01). BamHI 12.0 kb (P < 0.05) and 5.8 kb (P < 0.02, P < 0.02), EcoRI 20 kb (P < 0.05, P < 0.02), and Psti 6.0 kb (P < 0.05) fragments were more frequent in diabetic individuals compared with nonrelated control siblings or their parents, respectively. Analysis of individual haplotypes revealed that HLA-DR4-containing chromosomes were heterogeneous among controls but that the diabetic individuals showed a similar pattern of restriction fragment length polymorphism. Genomic blotting of blood lymphocyte DNA with a cDNA clone encoding the chain of HLA-D region class II antigens permits detection of fragments that are strongly associated with IDDM.
(Less)
- author
- Owerbach, D.
; Hagglof, B.
; Lernmark, A.
LU
and Holmgren, G.
- publishing date
- 1984-01-01
- type
- Contribution to journal
- publication status
- published
- in
- Diabetes
- volume
- 33
- issue
- 10
- pages
- 8 pages
- publisher
- American Diabetes Association Inc.
- external identifiers
-
- pmid:6090247
- scopus:0021200433
- ISSN
- 0012-1797
- DOI
- 10.2337/diab.33.10.958
- language
- English
- LU publication?
- no
- id
- 5c173074-7468-40f2-9ba2-ee93c2e0d837
- date added to LUP
- 2019-09-16 12:43:27
- date last changed
- 2024-03-13 08:13:54
@article{5c173074-7468-40f2-9ba2-ee93c2e0d837,
abstract = {{<p>DNA fragments complementary to cloned sequences encoding HLA-D region class II antigen α- and β-chains were determined by clotting with DNA from HLA-typed members of 22 complete families, 12 of which had a proband with insulin-dependent diabetes mellitus (IDDM). Analysis of genotypes showed that the DNA sequences were linked to HLA-DR and permitted confirmation of recombinations in two families. Digestion with the restriction enzymes BamHI, EcoRI, and Pstl and hybridization with an HLA-D region β-chain cDNA probe confirmed a BamHI 3.7 kilobase (kb) fragment present at low frequency among diabetic individuals and a BamHI 3.2 kb fragment that was also decreased among the diabetic subjects compared with siblings (P < 0.05) as well as nonrelated control siblings (P < 0.02) and their parents (P < 0.01). BamHI 12.0 kb (P < 0.05) and 5.8 kb (P < 0.02, P < 0.02), EcoRI 20 kb (P < 0.05, P < 0.02), and Psti 6.0 kb (P < 0.05) fragments were more frequent in diabetic individuals compared with nonrelated control siblings or their parents, respectively. Analysis of individual haplotypes revealed that HLA-DR4-containing chromosomes were heterogeneous among controls but that the diabetic individuals showed a similar pattern of restriction fragment length polymorphism. Genomic blotting of blood lymphocyte DNA with a cDNA clone encoding the chain of HLA-D region class II antigens permits detection of fragments that are strongly associated with IDDM.</p>}},
author = {{Owerbach, D. and Hagglof, B. and Lernmark, A. and Holmgren, G.}},
issn = {{0012-1797}},
language = {{eng}},
month = {{01}},
number = {{10}},
pages = {{958--965}},
publisher = {{American Diabetes Association Inc.}},
series = {{Diabetes}},
title = {{Susceptibility to insulin-dependent diabetes defined by restriction enzyme polymorphism of HLA-D region genomic DNA}},
url = {{http://dx.doi.org/10.2337/diab.33.10.958}},
doi = {{10.2337/diab.33.10.958}},
volume = {{33}},
year = {{1984}},
}