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Fermentation of the Straw Material Paja Brava by the Yeast Pichia stipitis in a Simultaneous Saccharification and Fermentation Process

Carrasco, Cristhian LU ; Baudel, Henrique; Roslander, Christian LU and Galbe, Mats LU (2013) In Journal of Sustainable Bioenergy Systems 3(2). p.99-106
Abstract
Paja Brava is a native South American grass with a high carbohydrate content. In the current work, the potential of us- ing this feedstock for ethanol production using a simultaneous saccharification and fermentation (SSF) process with the xylose-fermenting yeast Pichia stipitis (Scheffersomyces stipitis) CBS6054 was investigated. The straw material was subjected to SO2 catalyzed steam pretreatment at 200°C and 5 min residence time, which resulted in a solubilization of pentose sugars (mainly xylose) of 64% with only minor amounts of degradation products. The obtained material, in- cluding the pretreatment liquid, was subsequently hydrolyzed and fermented in an SSF process at microaerobic condi- tions using either a batch or a fed-batch... (More)
Paja Brava is a native South American grass with a high carbohydrate content. In the current work, the potential of us- ing this feedstock for ethanol production using a simultaneous saccharification and fermentation (SSF) process with the xylose-fermenting yeast Pichia stipitis (Scheffersomyces stipitis) CBS6054 was investigated. The straw material was subjected to SO2 catalyzed steam pretreatment at 200°C and 5 min residence time, which resulted in a solubilization of pentose sugars (mainly xylose) of 64% with only minor amounts of degradation products. The obtained material, in- cluding the pretreatment liquid, was subsequently hydrolyzed and fermented in an SSF process at microaerobic condi- tions using either a batch or a fed-batch process at a total water-insoluble solids loading of 10%. Overall yields of etha- nol based on all available sugars of 0.24 g/g and 0.27 g/g were obtained for batch and fed-batch mode of operation, re- spectively. The higher yield in the fed-batch process coincided with a higher degree of conversion of the sugars in the liquid medium, in particular of arabinose, for which the conversion was doubled (from 48% to 97%). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ethanol, Lignocellulose, Fermentation, Xylose
in
Journal of Sustainable Bioenergy Systems
volume
3
issue
2
pages
99 - 106
publisher
Scientific Research
ISSN
2165-4018
DOI
10.4236/jsbs.2013.32014
language
English
LU publication?
yes
id
5c61585e-c6e0-40b7-838a-9abd12667efa (old id 3918791)
date added to LUP
2013-07-08 10:18:48
date last changed
2018-05-29 11:54:28
@article{5c61585e-c6e0-40b7-838a-9abd12667efa,
  abstract     = {Paja Brava is a native South American grass with a high carbohydrate content. In the current work, the potential of us- ing this feedstock for ethanol production using a simultaneous saccharification and fermentation (SSF) process with the xylose-fermenting yeast Pichia stipitis (Scheffersomyces stipitis) CBS6054 was investigated. The straw material was subjected to SO2 catalyzed steam pretreatment at 200°C and 5 min residence time, which resulted in a solubilization of pentose sugars (mainly xylose) of 64% with only minor amounts of degradation products. The obtained material, in- cluding the pretreatment liquid, was subsequently hydrolyzed and fermented in an SSF process at microaerobic condi- tions using either a batch or a fed-batch process at a total water-insoluble solids loading of 10%. Overall yields of etha- nol based on all available sugars of 0.24 g/g and 0.27 g/g were obtained for batch and fed-batch mode of operation, re- spectively. The higher yield in the fed-batch process coincided with a higher degree of conversion of the sugars in the liquid medium, in particular of arabinose, for which the conversion was doubled (from 48% to 97%).},
  author       = {Carrasco, Cristhian and Baudel, Henrique and Roslander, Christian and Galbe, Mats},
  issn         = {2165-4018},
  keyword      = {Ethanol,Lignocellulose,Fermentation,Xylose},
  language     = {eng},
  number       = {2},
  pages        = {99--106},
  publisher    = {Scientific Research},
  series       = {Journal of Sustainable Bioenergy Systems},
  title        = {Fermentation of the Straw Material Paja Brava by the Yeast Pichia stipitis in a Simultaneous Saccharification and Fermentation Process},
  url          = {http://dx.doi.org/10.4236/jsbs.2013.32014},
  volume       = {3},
  year         = {2013},
}