Evaluation of 92 cardiovascular proteins in dried blood spots collected under field-conditions : Off-the-shelf affinity-based multiplexed assays work well, allowing for simplified sample collection
(2021) In BioEssays 43(9).- Abstract
Workplace-collected blood spots deposited on filter paper were analysed with multiplexed affinity-based protein assays and found to be suitable for proteomics analysis. The protein extension assay (PEA) was used to characterize 92 proteins using 1.2 mm punches in repeated samples collected from 20 workers. Overall, 97.8% of the samples and 91.3% of the analysed proteins passed quality control. Both within and between spot correlations using six replicates from the same individual were above 0.99, suggesting that comparable levels are obtained from multiple punches from the same spot and from consecutive spots. Protein levels from dried blood and wet serum from the same individuals were compared and the majority of the analysed proteins... (More)
Workplace-collected blood spots deposited on filter paper were analysed with multiplexed affinity-based protein assays and found to be suitable for proteomics analysis. The protein extension assay (PEA) was used to characterize 92 proteins using 1.2 mm punches in repeated samples collected from 20 workers. Overall, 97.8% of the samples and 91.3% of the analysed proteins passed quality control. Both within and between spot correlations using six replicates from the same individual were above 0.99, suggesting that comparable levels are obtained from multiple punches from the same spot and from consecutive spots. Protein levels from dried blood and wet serum from the same individuals were compared and the majority of the analysed proteins were found to be significantly correlated. These results open up for simplified sample collection of blood in field conditions for proteomic analysis, but also highlight that not all proteins can be robustly measured from dried whole blood.
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- author
- Broberg, Karin ; Svensson, Johanna LU ; Grahn, Karin ; Assarsson, Eva LU ; Åberg, Mikael ; Selander, Jenny and Enroth, Stefan
- organization
- publishing date
- 2021-02-15
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- affinity based proteomics, dried blood spots, sample collection
- in
- BioEssays
- volume
- 43
- issue
- 9
- article number
- 2000299
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:85100834616
- pmid:33586222
- ISSN
- 0265-9247
- DOI
- 10.1002/bies.202000299
- language
- English
- LU publication?
- yes
- id
- 5cfe6366-1ca9-4359-bb3b-e8eaa524095e
- date added to LUP
- 2021-03-04 12:51:19
- date last changed
- 2024-04-18 02:40:36
@article{5cfe6366-1ca9-4359-bb3b-e8eaa524095e,
abstract = {{<p>Workplace-collected blood spots deposited on filter paper were analysed with multiplexed affinity-based protein assays and found to be suitable for proteomics analysis. The protein extension assay (PEA) was used to characterize 92 proteins using 1.2 mm punches in repeated samples collected from 20 workers. Overall, 97.8% of the samples and 91.3% of the analysed proteins passed quality control. Both within and between spot correlations using six replicates from the same individual were above 0.99, suggesting that comparable levels are obtained from multiple punches from the same spot and from consecutive spots. Protein levels from dried blood and wet serum from the same individuals were compared and the majority of the analysed proteins were found to be significantly correlated. These results open up for simplified sample collection of blood in field conditions for proteomic analysis, but also highlight that not all proteins can be robustly measured from dried whole blood.</p>}},
author = {{Broberg, Karin and Svensson, Johanna and Grahn, Karin and Assarsson, Eva and Åberg, Mikael and Selander, Jenny and Enroth, Stefan}},
issn = {{0265-9247}},
keywords = {{affinity based proteomics; dried blood spots; sample collection}},
language = {{eng}},
month = {{02}},
number = {{9}},
publisher = {{John Wiley & Sons Inc.}},
series = {{BioEssays}},
title = {{Evaluation of 92 cardiovascular proteins in dried blood spots collected under field-conditions : Off-the-shelf affinity-based multiplexed assays work well, allowing for simplified sample collection}},
url = {{http://dx.doi.org/10.1002/bies.202000299}},
doi = {{10.1002/bies.202000299}},
volume = {{43}},
year = {{2021}},
}