A novel competitive capacitive glucose biosensor based on concanavalin A-labeled nanogold colloids assembled on a polytyramine-modified gold electrode.
(2010) In Analytica Chimica Acta 659(1-2). p.194-200- Abstract
- A highly sensitive competitive capacitive glucose biosensor was constructed based on gold nanoparticles, which were employed as a platform to immobilize concanavalin A (Con A). Gold nanoparticles were fixed on a gold electrode, on which a layer of polytyramine was preformed via electrochemical polymerization. The sensing mechanism is based on the competitive dissociation of a glucose polymer or a glycoconjugate from the glycoligand binding sites of immobilized Con A by the added glucose. To further improve the sensor response, several glucose polymers as well as a synthesized glycoconjugate using the periodate method, were screened. Consequently, dextran (MW 39 kDa) was selected and the feasibility of the proposed biosensor was evaluated... (More)
- A highly sensitive competitive capacitive glucose biosensor was constructed based on gold nanoparticles, which were employed as a platform to immobilize concanavalin A (Con A). Gold nanoparticles were fixed on a gold electrode, on which a layer of polytyramine was preformed via electrochemical polymerization. The sensing mechanism is based on the competitive dissociation of a glucose polymer or a glycoconjugate from the glycoligand binding sites of immobilized Con A by the added glucose. To further improve the sensor response, several glucose polymers as well as a synthesized glycoconjugate using the periodate method, were screened. Consequently, dextran (MW 39 kDa) was selected and the feasibility of the proposed biosensor was evaluated for a competitive assay of glucose. Experimental results show that the biosensor responded linearly to glucose in the range from 1.0 x 10(-6) to 1.0 x 10(-2) M, corresponding to 0.18 microg mL(-1) to 1.8 mg mL(-1) of glucose with a detection limit of 1.0 x 10(-6) M under optimized conditions. The studied biosensor exhibited a response time of about 15 min and a neglectable loss in sensitivity after 10 repeated analytical cycles. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1540575
- author
- Labib, Mahmoud LU ; Hedström, Martin LU ; Amin, Magdy and Mattiasson, Bo LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytica Chimica Acta
- volume
- 659
- issue
- 1-2
- pages
- 194 - 200
- publisher
- Elsevier
- external identifiers
-
- wos:000274046900025
- pmid:20103124
- scopus:72549098660
- pmid:20103124
- ISSN
- 1873-4324
- DOI
- 10.1016/j.aca.2009.11.028
- language
- English
- LU publication?
- yes
- id
- 5d0542f9-e8ef-429c-95e2-f8cb0e2df036 (old id 1540575)
- date added to LUP
- 2016-04-01 14:31:02
- date last changed
- 2022-03-29 21:24:46
@article{5d0542f9-e8ef-429c-95e2-f8cb0e2df036,
abstract = {{A highly sensitive competitive capacitive glucose biosensor was constructed based on gold nanoparticles, which were employed as a platform to immobilize concanavalin A (Con A). Gold nanoparticles were fixed on a gold electrode, on which a layer of polytyramine was preformed via electrochemical polymerization. The sensing mechanism is based on the competitive dissociation of a glucose polymer or a glycoconjugate from the glycoligand binding sites of immobilized Con A by the added glucose. To further improve the sensor response, several glucose polymers as well as a synthesized glycoconjugate using the periodate method, were screened. Consequently, dextran (MW 39 kDa) was selected and the feasibility of the proposed biosensor was evaluated for a competitive assay of glucose. Experimental results show that the biosensor responded linearly to glucose in the range from 1.0 x 10(-6) to 1.0 x 10(-2) M, corresponding to 0.18 microg mL(-1) to 1.8 mg mL(-1) of glucose with a detection limit of 1.0 x 10(-6) M under optimized conditions. The studied biosensor exhibited a response time of about 15 min and a neglectable loss in sensitivity after 10 repeated analytical cycles.}},
author = {{Labib, Mahmoud and Hedström, Martin and Amin, Magdy and Mattiasson, Bo}},
issn = {{1873-4324}},
language = {{eng}},
number = {{1-2}},
pages = {{194--200}},
publisher = {{Elsevier}},
series = {{Analytica Chimica Acta}},
title = {{A novel competitive capacitive glucose biosensor based on concanavalin A-labeled nanogold colloids assembled on a polytyramine-modified gold electrode.}},
url = {{http://dx.doi.org/10.1016/j.aca.2009.11.028}},
doi = {{10.1016/j.aca.2009.11.028}},
volume = {{659}},
year = {{2010}},
}