Combination of antibodies inhibits accelerated rejection mediated by memory T cells in xenoantigen-primed mice.
(2010) In Xenotransplantation 17(6). p.460-468- Abstract
- Wang F, Xia J, Chen J, Peng Y, Cheng P, Ekberg H, Wang X, Qi Z. Combination of antibodies inhibits accelerated rejection mediated by memory T cells in xenoantigen-primed mice. Xenotransplantation 2010; 17: 460-468. © 2010 John Wiley & Sons A/S. Abstract: Background: Donor-reactive memory T cells are known to accelerate allograft rejection; in our previous study, we reported that combined monoclonal antibodies (mAbs) could prolong islet allograft survival in alloantigen-primed mice. In this study, we examine the effects of donor-reactive memory T cells on the xenograft survival and methods to prolong the islet graft survival. Methods: To collect donor-reactive T cells, we performed full-thickness rat skin xenografting on BALB/c mice and... (More)
- Wang F, Xia J, Chen J, Peng Y, Cheng P, Ekberg H, Wang X, Qi Z. Combination of antibodies inhibits accelerated rejection mediated by memory T cells in xenoantigen-primed mice. Xenotransplantation 2010; 17: 460-468. © 2010 John Wiley & Sons A/S. Abstract: Background: Donor-reactive memory T cells are known to accelerate allograft rejection; in our previous study, we reported that combined monoclonal antibodies (mAbs) could prolong islet allograft survival in alloantigen-primed mice. In this study, we examine the effects of donor-reactive memory T cells on the xenograft survival and methods to prolong the islet graft survival. Methods: To collect donor-reactive T cells, we performed full-thickness rat skin xenografting on BALB/c mice and isolated the T cells from the mice after 6-8 weeks. These cells were then adoptively transferred to syngenic mice 1 day before rat-to-mouse islet transplantation. Three experimental groups were established in the adoptive transfer model: recipient mice treated with isotype mAbs (isotype group); mice treated with anti-CD40L mAb (anti-CD40L group); and mice treated with anti-CD40L, anti-OX40L, and anti-CD122 mAbs (3-combined group). Results: Lewis rat islet xenografts transplanted in naïve mice showed a mean survival time (MST) of 12.8 days, while the graft rejection was accelerated if the recipient mice were treated with adoptively transferred donor-reactive T cells (MST, 8.67 days). Treatment with anti-CD40L mb could not reverse the accelerated rejection (MST, 9.3 days). However, when anti-CD40L mb was combined with anti-OX40L and anti-CD122 mAbs, there was a considerable increase in the MST, which was 72.2 days. Compared to the isotype group, the 3-combined group had significantly lesser proportion of memory T cells and greater proportion of regulatory T cells (Tregs) in the spleen. Meanwhile, in the 3-combined group, the production of anti-rat antibodies was markedly inhibited. Conclusion: Treatment with a combination of antibodies could significantly reverse the accelerated rejection mediated by donor-reactive memory T cells by inhibiting cellular and humoral immune responses. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1756348
- author
- Wang, Feiyu ; Xia, Junjie ; Chen, Jibing ; Peng, Yuanzheng ; Cheng, Panpan ; Ekberg, Henrik LU ; Wang, Xiaomin and Qi, Zhongquan LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Xenotransplantation
- volume
- 17
- issue
- 6
- pages
- 460 - 468
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000285390200010
- pmid:21158947
- scopus:78650641856
- ISSN
- 0908-665X
- DOI
- 10.1111/j.1399-3089.2010.00618.x
- language
- English
- LU publication?
- yes
- id
- 5d0fc52b-b1dc-45ca-b342-c85736e3426a (old id 1756348)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21158947?dopt=Abstract
- date added to LUP
- 2016-04-04 09:35:38
- date last changed
- 2022-01-29 18:37:56
@article{5d0fc52b-b1dc-45ca-b342-c85736e3426a,
abstract = {{Wang F, Xia J, Chen J, Peng Y, Cheng P, Ekberg H, Wang X, Qi Z. Combination of antibodies inhibits accelerated rejection mediated by memory T cells in xenoantigen-primed mice. Xenotransplantation 2010; 17: 460-468. © 2010 John Wiley & Sons A/S. Abstract: Background: Donor-reactive memory T cells are known to accelerate allograft rejection; in our previous study, we reported that combined monoclonal antibodies (mAbs) could prolong islet allograft survival in alloantigen-primed mice. In this study, we examine the effects of donor-reactive memory T cells on the xenograft survival and methods to prolong the islet graft survival. Methods: To collect donor-reactive T cells, we performed full-thickness rat skin xenografting on BALB/c mice and isolated the T cells from the mice after 6-8 weeks. These cells were then adoptively transferred to syngenic mice 1 day before rat-to-mouse islet transplantation. Three experimental groups were established in the adoptive transfer model: recipient mice treated with isotype mAbs (isotype group); mice treated with anti-CD40L mAb (anti-CD40L group); and mice treated with anti-CD40L, anti-OX40L, and anti-CD122 mAbs (3-combined group). Results: Lewis rat islet xenografts transplanted in naïve mice showed a mean survival time (MST) of 12.8 days, while the graft rejection was accelerated if the recipient mice were treated with adoptively transferred donor-reactive T cells (MST, 8.67 days). Treatment with anti-CD40L mb could not reverse the accelerated rejection (MST, 9.3 days). However, when anti-CD40L mb was combined with anti-OX40L and anti-CD122 mAbs, there was a considerable increase in the MST, which was 72.2 days. Compared to the isotype group, the 3-combined group had significantly lesser proportion of memory T cells and greater proportion of regulatory T cells (Tregs) in the spleen. Meanwhile, in the 3-combined group, the production of anti-rat antibodies was markedly inhibited. Conclusion: Treatment with a combination of antibodies could significantly reverse the accelerated rejection mediated by donor-reactive memory T cells by inhibiting cellular and humoral immune responses.}},
author = {{Wang, Feiyu and Xia, Junjie and Chen, Jibing and Peng, Yuanzheng and Cheng, Panpan and Ekberg, Henrik and Wang, Xiaomin and Qi, Zhongquan}},
issn = {{0908-665X}},
language = {{eng}},
number = {{6}},
pages = {{460--468}},
publisher = {{Wiley-Blackwell}},
series = {{Xenotransplantation}},
title = {{Combination of antibodies inhibits accelerated rejection mediated by memory T cells in xenoantigen-primed mice.}},
url = {{http://dx.doi.org/10.1111/j.1399-3089.2010.00618.x}},
doi = {{10.1111/j.1399-3089.2010.00618.x}},
volume = {{17}},
year = {{2010}},
}