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Profiling dendritic cell subsets in head and neck squamous cell tonsillar cancer and benign tonsils.

Abolhalaj, Milad LU ; Askmyr, David LU ; Sakellariou, Christina LU ; Lundberg, Kristina LU ; Greiff, Lennart LU and Lindstedt, Malin LU (2018) In Scientific Reports 8(8030).
Abstract
Dendritic cells (DCs) have a key role in orchestrating immune responses and are considered important targets for immunotherapy against cancer. In order to develop effective cancer vaccines, detailed knowledge of the micromilieu in cancer lesions is warranted. In this study, flow cytometry and human transcriptome arrays were used to characterize subsets of DCs in head and neck squamous cell tonsillar cancer and compare them to their counterparts in benign tonsils to evaluate subset-selective biomarkers associated with tonsillar cancer. We describe, for the first time, four subsets of DCs in tonsillar cancer: CD123+ plasmacytoid DCs (pDC), CD1c+, CD141+, and CD1c-CD141- myeloid DCs (mDC). An increased frequency of DCs and an elevated mDC/pDC... (More)
Dendritic cells (DCs) have a key role in orchestrating immune responses and are considered important targets for immunotherapy against cancer. In order to develop effective cancer vaccines, detailed knowledge of the micromilieu in cancer lesions is warranted. In this study, flow cytometry and human transcriptome arrays were used to characterize subsets of DCs in head and neck squamous cell tonsillar cancer and compare them to their counterparts in benign tonsils to evaluate subset-selective biomarkers associated with tonsillar cancer. We describe, for the first time, four subsets of DCs in tonsillar cancer: CD123+ plasmacytoid DCs (pDC), CD1c+, CD141+, and CD1c-CD141- myeloid DCs (mDC). An increased frequency of DCs and an elevated mDC/pDC ratio were shown in malignant compared to benign tonsillar tissue. The microarray data demonstrates characteristics specific for tonsil cancer DC subsets, including expression of immunosuppressive molecules and lower expression levels of genes involved in development of effector immune responses in DCs in malignant tonsillar tissue, compared to their counterparts in benign tonsillar tissue. Finally, we present target candidates selectively expressed by different DC subsets in malignant tonsils and confirm expression of CD206/MRC1 and CD207/Langerin on CD1c+ DCs at protein level. This study descibes DC characteristics in the context of head and neck cancer and add valuable steps towards future DC-based therapies against tonsillar cancer. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cancer microenvironment, Head and neck cancer, Immunotherapy, Tumour immunology
in
Scientific Reports
volume
8
issue
8030
publisher
Nature Publishing Group
external identifiers
  • scopus:85047509524
ISSN
2045-2322
DOI
10.1038/s41598-018-26193-y
language
English
LU publication?
yes
id
5d456b66-560c-4c56-bc56-34281b70a569
date added to LUP
2018-11-22 11:14:30
date last changed
2019-05-14 04:48:07
@article{5d456b66-560c-4c56-bc56-34281b70a569,
  abstract     = {Dendritic cells (DCs) have a key role in orchestrating immune responses and are considered important targets for immunotherapy against cancer. In order to develop effective cancer vaccines, detailed knowledge of the micromilieu in cancer lesions is warranted. In this study, flow cytometry and human transcriptome arrays were used to characterize subsets of DCs in head and neck squamous cell tonsillar cancer and compare them to their counterparts in benign tonsils to evaluate subset-selective biomarkers associated with tonsillar cancer. We describe, for the first time, four subsets of DCs in tonsillar cancer: CD123+ plasmacytoid DCs (pDC), CD1c+, CD141+, and CD1c-CD141- myeloid DCs (mDC). An increased frequency of DCs and an elevated mDC/pDC ratio were shown in malignant compared to benign tonsillar tissue. The microarray data demonstrates characteristics specific for tonsil cancer DC subsets, including expression of immunosuppressive molecules and lower expression levels of genes involved in development of effector immune responses in DCs in malignant tonsillar tissue, compared to their counterparts in benign tonsillar tissue. Finally, we present target candidates selectively expressed by different DC subsets in malignant tonsils and confirm expression of CD206/MRC1 and CD207/Langerin on CD1c+ DCs at protein level. This study descibes DC characteristics in the context of head and neck cancer and add valuable steps towards future DC-based therapies against tonsillar cancer.},
  articleno    = {8030},
  author       = {Abolhalaj, Milad and Askmyr, David and Sakellariou, Christina and Lundberg, Kristina and Greiff, Lennart and Lindstedt, Malin},
  issn         = {2045-2322},
  keyword      = {Cancer microenvironment,Head and neck cancer,Immunotherapy,Tumour immunology},
  language     = {eng},
  month        = {05},
  number       = {8030},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Profiling dendritic cell subsets in head and neck squamous cell tonsillar cancer and benign tonsils.},
  url          = {http://dx.doi.org/10.1038/s41598-018-26193-y},
  volume       = {8},
  year         = {2018},
}