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High-performance liquid affinity chromatography on silica-bound concanavalin A

Borchert, Axel ; Larsson, Per-Olof LU and Mosbach, Klaus LU (1982) In Journal of chromatography. A 244. p.49-56
Abstract
Concanavalin A was covalently bound to porous silica (100-1000 Å) by three different coupling procedures. The performance of the preparations was evaluated from their ability to resolve a mixture of closely related sugars. The Concanavalin A-silica was used for efficient and rapid (5-20 min) analysis/purification of samples containing the glucoproteins peroxidase and glucose oxidase. Such columns could be used at least 60 times with retained performance.
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of chromatography. A
volume
244
pages
8 pages
publisher
Elsevier
external identifiers
  • scopus:0008726721
ISSN
0021-9673
DOI
10.1016/S0021-9673(00)80121-1
language
English
LU publication?
yes
id
5d560a5c-ebbf-451f-ab13-0d989c4dc671
date added to LUP
2024-06-18 19:59:30
date last changed
2025-04-04 14:10:43
@article{5d560a5c-ebbf-451f-ab13-0d989c4dc671,
  abstract     = {{Concanavalin A was covalently bound to porous silica (100-1000 Å) by three different coupling procedures. The performance of the preparations was evaluated from their ability to resolve a mixture of closely related sugars. The Concanavalin A-silica was used for efficient and rapid (5-20 min) analysis/purification of samples containing the glucoproteins peroxidase and glucose oxidase. Such columns could be used at least 60 times with retained performance.}},
  author       = {{Borchert, Axel and Larsson, Per-Olof and Mosbach, Klaus}},
  issn         = {{0021-9673}},
  language     = {{eng}},
  pages        = {{49--56}},
  publisher    = {{Elsevier}},
  series       = {{Journal of chromatography. A}},
  title        = {{High-performance liquid affinity chromatography on silica-bound concanavalin A}},
  url          = {{http://dx.doi.org/10.1016/S0021-9673(00)80121-1}},
  doi          = {{10.1016/S0021-9673(00)80121-1}},
  volume       = {{244}},
  year         = {{1982}},
}