Glucose sensors with increased sensitivity based on composite gels containing immobilized boronic acid
(2008) In Reactive & Functional Polymers 68(12). p.1625-1635- Abstract
- Copolymerization of N-acryloyl-m-aminophenylboronic acid (NAAPBA) with acrylamide (AAm) on the surface of glass plates resulted in the formation of a hydrogel film with porous structure and capable of optical response to the changes in concentration of glucose in the contacting solution. The boronate specific dye, Alizarin Red S (ARS) was incorporated into hydrogel film to increase the sensitivity towards glucose and poly(N-vinylpyrrolidone) (PVP) was incorporated to prevent the dye leakage. The obtained boronate-containing polymer film responded to submicromolar concentration Of glucose with optical density changes being a linear function of glucose concentration in the range from 0.1 to 1 mM. The PVP-ARS-containing gel exhibited a... (More)
- Copolymerization of N-acryloyl-m-aminophenylboronic acid (NAAPBA) with acrylamide (AAm) on the surface of glass plates resulted in the formation of a hydrogel film with porous structure and capable of optical response to the changes in concentration of glucose in the contacting solution. The boronate specific dye, Alizarin Red S (ARS) was incorporated into hydrogel film to increase the sensitivity towards glucose and poly(N-vinylpyrrolidone) (PVP) was incorporated to prevent the dye leakage. The obtained boronate-containing polymer film responded to submicromolar concentration Of glucose with optical density changes being a linear function of glucose concentration in the range from 0.1 to 1 mM. The PVP-ARS-containing gel exhibited a 10-fold higher glucose sensitivity compared with the colorless NAAPBA-AAm gel. The equilibrium dissociation constants of soluble ARS-PVP and ARS-NAAPBA-containing copolymer complexes at pH 7.3 were found to be 1.4 and 0.44 mu M, respectively. The low dissociation constants enabled the preparation of the sensor with increased operational stability of at least two months allowing more than 20 assays of with no loss in the sensitivity or dye leakage. (C) 2008 Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1376364
- author
- Hajizadeh, Solmaz LU ; Ivanov, Alexander LU ; Jahanshahi, Mohsen ; Sanati, Mohammad Hossein ; Zhuravleva, Natalia V. ; Mikhalovska, Lyubov I. and Galaev, Igor LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Composite hydrogel, Phenylboronic acid, Smart polymers, Optical response
- in
- Reactive & Functional Polymers
- volume
- 68
- issue
- 12
- pages
- 1625 - 1635
- publisher
- Elsevier
- external identifiers
-
- wos:000261872700004
- scopus:55949123609
- ISSN
- 1873-166X
- DOI
- 10.1016/j.reactfunctpolym.2008.09.006
- language
- English
- LU publication?
- yes
- id
- 5dd2484c-1030-4a52-b59e-1187bb8ba971 (old id 1376364)
- date added to LUP
- 2016-04-01 11:39:18
- date last changed
- 2023-08-24 12:37:34
@article{5dd2484c-1030-4a52-b59e-1187bb8ba971, abstract = {{Copolymerization of N-acryloyl-m-aminophenylboronic acid (NAAPBA) with acrylamide (AAm) on the surface of glass plates resulted in the formation of a hydrogel film with porous structure and capable of optical response to the changes in concentration of glucose in the contacting solution. The boronate specific dye, Alizarin Red S (ARS) was incorporated into hydrogel film to increase the sensitivity towards glucose and poly(N-vinylpyrrolidone) (PVP) was incorporated to prevent the dye leakage. The obtained boronate-containing polymer film responded to submicromolar concentration Of glucose with optical density changes being a linear function of glucose concentration in the range from 0.1 to 1 mM. The PVP-ARS-containing gel exhibited a 10-fold higher glucose sensitivity compared with the colorless NAAPBA-AAm gel. The equilibrium dissociation constants of soluble ARS-PVP and ARS-NAAPBA-containing copolymer complexes at pH 7.3 were found to be 1.4 and 0.44 mu M, respectively. The low dissociation constants enabled the preparation of the sensor with increased operational stability of at least two months allowing more than 20 assays of with no loss in the sensitivity or dye leakage. (C) 2008 Elsevier Ltd. All rights reserved.}}, author = {{Hajizadeh, Solmaz and Ivanov, Alexander and Jahanshahi, Mohsen and Sanati, Mohammad Hossein and Zhuravleva, Natalia V. and Mikhalovska, Lyubov I. and Galaev, Igor}}, issn = {{1873-166X}}, keywords = {{Composite hydrogel; Phenylboronic acid; Smart polymers; Optical response}}, language = {{eng}}, number = {{12}}, pages = {{1625--1635}}, publisher = {{Elsevier}}, series = {{Reactive & Functional Polymers}}, title = {{Glucose sensors with increased sensitivity based on composite gels containing immobilized boronic acid}}, url = {{http://dx.doi.org/10.1016/j.reactfunctpolym.2008.09.006}}, doi = {{10.1016/j.reactfunctpolym.2008.09.006}}, volume = {{68}}, year = {{2008}}, }