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Annexin A2 antibodies but not inhibitors of the annexin A2 heterotetramer impair productive HIV-1 infection of macrophages in vitro

Woodham, Andrew W.; Sanna, Adriana M. LU ; Taylor, Julia R.; Skeate, Joseph G.; Da Silva, Diane M.; Dekker, Lodewijk V. and Kast, W. Martin (2016) In Virology Journal 13(1).
Abstract

During sexual transmission of human immunodeficiency virus (HIV), macrophages are initial targets for HIV infection. Secretory leukocyte protease inhibitor (SLPI) has been shown to protect against HIV infection of macrophages through interactions with annexin A2 (A2), which is found on the macrophage cell surface as a heterotetramer (A2t) consisting of A2 and S100A10. Therefore, we investigated potential protein-protein interactions between A2 and HIV-1 gp120 through a series of co-immunoprecipitation assays and a single molecule pulldown (SiMPull) technique. Additionally, inhibitors of A2t (A2ti) that target the interaction between A2 and S100A10 were tested for their ability to impair productive HIV-1 infection of macrophages. Our... (More)

During sexual transmission of human immunodeficiency virus (HIV), macrophages are initial targets for HIV infection. Secretory leukocyte protease inhibitor (SLPI) has been shown to protect against HIV infection of macrophages through interactions with annexin A2 (A2), which is found on the macrophage cell surface as a heterotetramer (A2t) consisting of A2 and S100A10. Therefore, we investigated potential protein-protein interactions between A2 and HIV-1 gp120 through a series of co-immunoprecipitation assays and a single molecule pulldown (SiMPull) technique. Additionally, inhibitors of A2t (A2ti) that target the interaction between A2 and S100A10 were tested for their ability to impair productive HIV-1 infection of macrophages. Our data suggest that interactions between HIV-1 gp120 and A2 exist, though this interaction may be indirect. Furthermore, an anti-A2 antibody impaired HIV-1 particle production in macrophages in vitro, whereas A2ti did not indicating that annexin A2 may promote HIV-1 infection of macrophages in its monomeric rather than tetrameric form.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Annexin A2, Annexin A2 heterotetramer, HIV-1, Inhibitor, Macrophage, Receptor
in
Virology Journal
volume
13
issue
1
pages
5 pages
publisher
BioMed Central
external identifiers
  • scopus:84995546010
  • wos:000388038300001
ISSN
1743-422X
DOI
10.1186/s12985-016-0649-5
language
English
LU publication?
yes
id
5de22a0f-b06f-4b4a-83a2-7e107bc6dcdb
date added to LUP
2016-12-02 14:38:00
date last changed
2017-03-10 09:04:19
@article{5de22a0f-b06f-4b4a-83a2-7e107bc6dcdb,
  abstract     = {<p>During sexual transmission of human immunodeficiency virus (HIV), macrophages are initial targets for HIV infection. Secretory leukocyte protease inhibitor (SLPI) has been shown to protect against HIV infection of macrophages through interactions with annexin A2 (A2), which is found on the macrophage cell surface as a heterotetramer (A2t) consisting of A2 and S100A10. Therefore, we investigated potential protein-protein interactions between A2 and HIV-1 gp120 through a series of co-immunoprecipitation assays and a single molecule pulldown (SiMPull) technique. Additionally, inhibitors of A2t (A2ti) that target the interaction between A2 and S100A10 were tested for their ability to impair productive HIV-1 infection of macrophages. Our data suggest that interactions between HIV-1 gp120 and A2 exist, though this interaction may be indirect. Furthermore, an anti-A2 antibody impaired HIV-1 particle production in macrophages in vitro, whereas A2ti did not indicating that annexin A2 may promote HIV-1 infection of macrophages in its monomeric rather than tetrameric form.</p>},
  author       = {Woodham, Andrew W. and Sanna, Adriana M. and Taylor, Julia R. and Skeate, Joseph G. and Da Silva, Diane M. and Dekker, Lodewijk V. and Kast, W. Martin},
  issn         = {1743-422X},
  keyword      = {Annexin A2,Annexin A2 heterotetramer,HIV-1,Inhibitor,Macrophage,Receptor},
  language     = {eng},
  month        = {11},
  number       = {1},
  pages        = {5},
  publisher    = {BioMed Central},
  series       = {Virology Journal},
  title        = {Annexin A2 antibodies but not inhibitors of the annexin A2 heterotetramer impair productive HIV-1 infection of macrophages in vitro},
  url          = {http://dx.doi.org/10.1186/s12985-016-0649-5},
  volume       = {13},
  year         = {2016},
}