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Simulated moving bed technology with a simplified approach for protein purification - Separation of lactoperoxidase and lactoferrin from whey protein concentrate

Andersson, Jonatan LU and Mattiasson, Bo LU (2006) In Journal of Chromatography A 1107(1-2). p.88-95
Abstract
Simulated moving bed (SMB) technology is a continuous chromatographic technique proven to have many advantages compared to conventional batch chromatography, such as: raised productivity and product concentration, reduced buffer consumption as well as more efficient use of raw material. In this study a 20 column SMB process for the separation of lactoperoxidase and lactoferrin from whey protein concentrate (WPC) was developed. A simplified approach with data from a single column experiment was used when designing the process. The SMB process data were compared to a theoretical scale-up of the breakthrough experiment reflecting the same 20 column set-up run in non-moving bed mode. The outcome of the comparison is a 48% raise in... (More)
Simulated moving bed (SMB) technology is a continuous chromatographic technique proven to have many advantages compared to conventional batch chromatography, such as: raised productivity and product concentration, reduced buffer consumption as well as more efficient use of raw material. In this study a 20 column SMB process for the separation of lactoperoxidase and lactoferrin from whey protein concentrate (WPC) was developed. A simplified approach with data from a single column experiment was used when designing the process. The SMB process data were compared to a theoretical scale-up of the breakthrough experiment reflecting the same 20 column set-up run in non-moving bed mode. The outcome of the comparison is a 48% raise in productivity, a 4.3 times decrease in buffer consumption, 6.5 times raise in target protein concentration with a raw material utilization which is slightly better for the SMB process. (Less)
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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cation exchange, whey, WPC, lactoferrin, lactoperoxidase, separation, protein, simulated moving bed, SMB
in
Journal of Chromatography A
volume
1107
issue
1-2
pages
88 - 95
publisher
Elsevier
external identifiers
  • pmid:16387313
  • wos:000235505100011
  • scopus:31844449572
ISSN
0021-9673
DOI
10.1016/j.chroma.2005.12.018
language
English
LU publication?
yes
id
5de30997-4e99-49a4-8f88-5d7263fd8d18 (old id 417121)
date added to LUP
2016-04-01 16:16:24
date last changed
2025-10-14 10:19:05
@article{5de30997-4e99-49a4-8f88-5d7263fd8d18,
  abstract     = {{Simulated moving bed (SMB) technology is a continuous chromatographic technique proven to have many advantages compared to conventional batch chromatography, such as: raised productivity and product concentration, reduced buffer consumption as well as more efficient use of raw material. In this study a 20 column SMB process for the separation of lactoperoxidase and lactoferrin from whey protein concentrate (WPC) was developed. A simplified approach with data from a single column experiment was used when designing the process. The SMB process data were compared to a theoretical scale-up of the breakthrough experiment reflecting the same 20 column set-up run in non-moving bed mode. The outcome of the comparison is a 48% raise in productivity, a 4.3 times decrease in buffer consumption, 6.5 times raise in target protein concentration with a raw material utilization which is slightly better for the SMB process.}},
  author       = {{Andersson, Jonatan and Mattiasson, Bo}},
  issn         = {{0021-9673}},
  keywords     = {{cation exchange; whey; WPC; lactoferrin; lactoperoxidase; separation; protein; simulated moving bed; SMB}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{88--95}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Chromatography A}},
  title        = {{Simulated moving bed technology with a simplified approach for protein purification - Separation of lactoperoxidase and lactoferrin from whey protein concentrate}},
  url          = {{http://dx.doi.org/10.1016/j.chroma.2005.12.018}},
  doi          = {{10.1016/j.chroma.2005.12.018}},
  volume       = {{1107}},
  year         = {{2006}},
}