Polyelectrolyte complexes as a tool for purification of plasmid DNA background and development
(2004) In Journal of Chromatography. B 807(1). p.121-127- Abstract
- The demand for highly purified plasmids in gene therapy and plasmid-based vaccines requires large-scale production of pharmaceutical-grade plasmid. Plasmid DNA was selectively precipitated from a clarified alkaline lysate using the polycation poly(N,N'-dimethyldiallylammonium) chloride which formed insoluble polyelectrolyte complex (PEC) with the plasmid DNA. Soluble PECs of DNA with polycations have earlier been used for cell transformation, but now the focus has been on insoluble PECs. Both DNA and RNA form stable PECs with synthetic polycations. However, it was possible to find a range of salt concentration where plasmid DNA was quantitatively precipitated whereas RNA remained in solution. The precipitated plasmid DNA was resolubilised... (More)
- The demand for highly purified plasmids in gene therapy and plasmid-based vaccines requires large-scale production of pharmaceutical-grade plasmid. Plasmid DNA was selectively precipitated from a clarified alkaline lysate using the polycation poly(N,N'-dimethyldiallylammonium) chloride which formed insoluble polyelectrolyte complex (PEC) with the plasmid DNA. Soluble PECs of DNA with polycations have earlier been used for cell transformation, but now the focus has been on insoluble PECs. Both DNA and RNA form stable PECs with synthetic polycations. However, it was possible to find a range of salt concentration where plasmid DNA was quantitatively precipitated whereas RNA remained in solution. The precipitated plasmid DNA was resolubilised at high salt concentration and the polycation was removed by gel-filtration. (C) 2004 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/140658
- author
- Wahlund, Per-Olof LU ; Gustavsson, Per-Erik LU ; Izumrudov, Vladimir LU ; Larsson, Per-Olof LU and Galaev, Igor LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Purification, Plasmid DNA, Polyelectrolyte complexes
- in
- Journal of Chromatography. B
- volume
- 807
- issue
- 1
- pages
- 121 - 127
- publisher
- Elsevier
- external identifiers
-
- wos:000222047600018
- pmid:15177169
- scopus:2642571617
- ISSN
- 1873-376X
- DOI
- 10.1016/j.jchromb.2004.01.046
- language
- English
- LU publication?
- yes
- id
- 5de49637-2174-4bd9-8909-e46de330a125 (old id 140658)
- date added to LUP
- 2016-04-01 11:47:45
- date last changed
- 2022-02-10 21:36:37
@article{5de49637-2174-4bd9-8909-e46de330a125, abstract = {{The demand for highly purified plasmids in gene therapy and plasmid-based vaccines requires large-scale production of pharmaceutical-grade plasmid. Plasmid DNA was selectively precipitated from a clarified alkaline lysate using the polycation poly(N,N'-dimethyldiallylammonium) chloride which formed insoluble polyelectrolyte complex (PEC) with the plasmid DNA. Soluble PECs of DNA with polycations have earlier been used for cell transformation, but now the focus has been on insoluble PECs. Both DNA and RNA form stable PECs with synthetic polycations. However, it was possible to find a range of salt concentration where plasmid DNA was quantitatively precipitated whereas RNA remained in solution. The precipitated plasmid DNA was resolubilised at high salt concentration and the polycation was removed by gel-filtration. (C) 2004 Elsevier B.V. All rights reserved.}}, author = {{Wahlund, Per-Olof and Gustavsson, Per-Erik and Izumrudov, Vladimir and Larsson, Per-Olof and Galaev, Igor}}, issn = {{1873-376X}}, keywords = {{Purification; Plasmid DNA; Polyelectrolyte complexes}}, language = {{eng}}, number = {{1}}, pages = {{121--127}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography. B}}, title = {{Polyelectrolyte complexes as a tool for purification of plasmid DNA background and development}}, url = {{http://dx.doi.org/10.1016/j.jchromb.2004.01.046}}, doi = {{10.1016/j.jchromb.2004.01.046}}, volume = {{807}}, year = {{2004}}, }