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A method for assessment of exposure to rubber glove allergens

Bergendorff, O. LU and Persson, Christina LU (2012) 11th Congress of the European Society of Contact Dermatitis (ESCD) In Contact Dermatitis 66(s2). p.70-71
Abstract
Background: Use of disposable protective gloves in health care is necessary both to protect patients and personnel. However, the use might also be responsible for skin disease among persons wearing the gloves. Dermatitis may be caused by additives in the gloves, either from manufacturing of the glove material or components in coatings applied after vulcanization. Objectives: We wanted to analyse to what extent chemicals were deposited to skin from the glove and to compare these results with analysis of the glove itself. Methods: After usage of protective gloves, the hands are washed with an ethanolic water solution in a plastic bag for 1 min. The wash solution is then analysed by different HPLC-methods to determine how much of each rubber... (More)
Background: Use of disposable protective gloves in health care is necessary both to protect patients and personnel. However, the use might also be responsible for skin disease among persons wearing the gloves. Dermatitis may be caused by additives in the gloves, either from manufacturing of the glove material or components in coatings applied after vulcanization. Objectives: We wanted to analyse to what extent chemicals were deposited to skin from the glove and to compare these results with analysis of the glove itself. Methods: After usage of protective gloves, the hands are washed with an ethanolic water solution in a plastic bag for 1 min. The wash solution is then analysed by different HPLC-methods to determine how much of each rubber allergen that has been left on the skin surface. Similar gloves are analysed by traditional methods by extraction with organic solvents to see which rubber allergens that are present in the glove. Results: Two different HPLC-methods were then chosen for analysis of the wash solution. Firstly a cyano-column was used for analysis of basic analytes like cetylpyridinium chloride and diphenylguanidine and secondly a nickel salt derivatisation stepwas used to stabilise dithiocarbamates for analysis on a C18 column. In the latter system thiurams and mercaptobenzothiazoles were analysed simultaneously. Conclusions: By this mild and non-invasive method we could analyse dermal exposure to the most important allergens in rubber gloves. The method enables us to assess how for example different working procedures before and during the use of protective gloves will influence on the amounts of chemicals that are deposited on the skin from different gloves. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
rubber, allergen, cetylpyridinium salt, organic solvent, plastic, water, nickel, glove, society, contact dermatitis, exposure, human, protective clothing, skin, extraction, patient, high performance liquid chromatography, skin surface, dermatitis, procedures, non invasive procedure, skin disease, personnel, health care
in
Contact Dermatitis
volume
66
issue
s2
pages
70 - 71
publisher
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
conference name
11th Congress of the European Society of Contact Dermatitis (ESCD)
ISSN
0105-1873
DOI
10.1111/j.1600-0536.2012.02113.x
language
English
LU publication?
yes
id
5e0b4708-1cc2-4e6d-97a2-7c1bf678663c
date added to LUP
2017-06-15 13:34:37
date last changed
2017-06-22 13:38:42
@misc{5e0b4708-1cc2-4e6d-97a2-7c1bf678663c,
  abstract     = {Background: Use of disposable protective gloves in health care is necessary both to protect patients and personnel. However, the use might also be responsible for skin disease among persons wearing the gloves. Dermatitis may be caused by additives in the gloves, either from manufacturing of the glove material or components in coatings applied after vulcanization. Objectives: We wanted to analyse to what extent chemicals were deposited to skin from the glove and to compare these results with analysis of the glove itself. Methods: After usage of protective gloves, the hands are washed with an ethanolic water solution in a plastic bag for 1 min. The wash solution is then analysed by different HPLC-methods to determine how much of each rubber allergen that has been left on the skin surface. Similar gloves are analysed by traditional methods by extraction with organic solvents to see which rubber allergens that are present in the glove. Results: Two different HPLC-methods were then chosen for analysis of the wash solution. Firstly a cyano-column was used for analysis of basic analytes like cetylpyridinium chloride and diphenylguanidine and secondly a nickel salt derivatisation stepwas used to stabilise dithiocarbamates for analysis on a C18 column. In the latter system thiurams and mercaptobenzothiazoles were analysed simultaneously. Conclusions: By this mild and non-invasive method we could analyse dermal exposure to the most important allergens in rubber gloves. The method enables us to assess how for example different working procedures before and during the use of protective gloves will influence on the amounts of chemicals that are deposited on the skin from different gloves.},
  articleno    = {P14},
  author       = {Bergendorff, O. and Persson, Christina},
  issn         = {0105-1873},
  keyword      = {rubber,allergen,cetylpyridinium salt,organic solvent,plastic,water,nickel,glove,society,contact dermatitis,exposure,human,protective clothing,skin,extraction,patient,high performance liquid chromatography,skin surface,dermatitis,procedures,non invasive procedure,skin disease,personnel,health care},
  language     = {eng},
  month        = {06},
  note         = {Conference Abstract},
  number       = {s2},
  pages        = {70--71},
  publisher    = {Federation of European Neuroscience Societies and Blackwell Publishing Ltd},
  series       = {Contact Dermatitis},
  title        = {A method for assessment of exposure to rubber glove allergens},
  url          = {http://dx.doi.org/10.1111/j.1600-0536.2012.02113.x},
  volume       = {66},
  year         = {2012},
}