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Evaluation of a novel radioimmunoassay using 125I-labelled human recombinant GAD65 for the determination of glutamic acid decarboxylase (GAD65) autoantibodies

Marcovina, Santica M.; Landin-Olsson, M. LU ; Essen-Möller, A.; Palmer, J. P. and Lernmark, Å LU (2000) In International Journal of Clinical and Laboratory Research 30(1). p.21-26
Abstract

Autoantibodies to the islet cell 65-kilodalton isoform of glutamic acid decarboxylase (GAD65) are present in most patients with type 1 diabetes mellitus years before the clinical manifestation of the disease. GAD65 autoantibodies are also present in a subset of patients with type 2 diabetes who frequently become insulin dependent. In the present study, we evaluated a new, commercially available radioimmunoprecipitation assay for measuring GAD65 autoantibodies using 125I-labelled human recombinant GAD65. Results obtained with this assay were compared with those obtained by a reference assay based on 35S-labelled recombinant GAD65. Analyses were performed on 67 patients with type 1 diabetes, 350 with type 2 diabetes,... (More)

Autoantibodies to the islet cell 65-kilodalton isoform of glutamic acid decarboxylase (GAD65) are present in most patients with type 1 diabetes mellitus years before the clinical manifestation of the disease. GAD65 autoantibodies are also present in a subset of patients with type 2 diabetes who frequently become insulin dependent. In the present study, we evaluated a new, commercially available radioimmunoprecipitation assay for measuring GAD65 autoantibodies using 125I-labelled human recombinant GAD65. Results obtained with this assay were compared with those obtained by a reference assay based on 35S-labelled recombinant GAD65. Analyses were performed on 67 patients with type 1 diabetes, 350 with type 2 diabetes, and 150 apparently healthy individuals. An excellent agreement was found between the results obtained by the 125-GAD65 assay and those obtained by the reference method. The receiver operating characteristic (ROC) curve analysis was used to evaluate the sensitivity and specificity of the two assays. The sensitivity of each assay was determined from the results of the 67 type 1 patients, while the specificity was based on the 150 healthy individuals. Based on the ROC curves, the two assays appeared identical, with a sensitivity of 84% and a clinical specificity of 98%. In conclusion, based on our results, this simple, one-step centrifugation, high-capacity 125I-GAD65 assay has the same sensitivity and specificity as the reference assay and is highly suitable to detect GAD65 autoantibodies in human samples.

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author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Autoantibodies, Diabetes mellitus, Glutamic acid decarboxylase, Radioimmunoassay, Receiver operating characteristic curve
in
International Journal of Clinical and Laboratory Research
volume
30
issue
1
pages
6 pages
external identifiers
  • scopus:0033920387
ISSN
0940-5437
DOI
10.1007/s005990070029
language
English
LU publication?
no
id
5e3efd56-b50e-44ff-a9e3-aa88c59a7999
date added to LUP
2017-09-07 09:03:03
date last changed
2017-09-07 09:03:03
@article{5e3efd56-b50e-44ff-a9e3-aa88c59a7999,
  abstract     = {<p>Autoantibodies to the islet cell 65-kilodalton isoform of glutamic acid decarboxylase (GAD65) are present in most patients with type 1 diabetes mellitus years before the clinical manifestation of the disease. GAD65 autoantibodies are also present in a subset of patients with type 2 diabetes who frequently become insulin dependent. In the present study, we evaluated a new, commercially available radioimmunoprecipitation assay for measuring GAD65 autoantibodies using <sup>125</sup>I-labelled human recombinant GAD65. Results obtained with this assay were compared with those obtained by a reference assay based on <sup>35</sup>S-labelled recombinant GAD65. Analyses were performed on 67 patients with type 1 diabetes, 350 with type 2 diabetes, and 150 apparently healthy individuals. An excellent agreement was found between the results obtained by the <sup>125</sup>-GAD65 assay and those obtained by the reference method. The receiver operating characteristic (ROC) curve analysis was used to evaluate the sensitivity and specificity of the two assays. The sensitivity of each assay was determined from the results of the 67 type 1 patients, while the specificity was based on the 150 healthy individuals. Based on the ROC curves, the two assays appeared identical, with a sensitivity of 84% and a clinical specificity of 98%. In conclusion, based on our results, this simple, one-step centrifugation, high-capacity <sup>125</sup>I-GAD65 assay has the same sensitivity and specificity as the reference assay and is highly suitable to detect GAD65 autoantibodies in human samples.</p>},
  author       = {Marcovina, Santica M. and Landin-Olsson, M. and Essen-Möller, A. and Palmer, J. P. and Lernmark, Å},
  issn         = {0940-5437},
  keyword      = {Autoantibodies,Diabetes mellitus,Glutamic acid decarboxylase,Radioimmunoassay,Receiver operating characteristic curve},
  language     = {eng},
  number       = {1},
  pages        = {21--26},
  series       = {International Journal of Clinical and Laboratory Research},
  title        = {Evaluation of a novel radioimmunoassay using <sup>125</sup>I-labelled human recombinant GAD65 for the determination of glutamic acid decarboxylase (GAD65) autoantibodies},
  url          = {http://dx.doi.org/10.1007/s005990070029},
  volume       = {30},
  year         = {2000},
}